Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from kidney by acetone precipitation, ion exchange chromatography, and gel filtration to homogeneity | Sus scrofa |
Renatured (Comment) | Organism |
---|---|
denaturation of the enzyme with 6 M guanidinium hydrochloride, dilution 60fold with Tris-HCl buffer, pH 7.5, determination of the refolding intermediate of guanidine hydrochloride denatured aminoacylase, the refolded enzyme shows 58-24% of maximal activity dependent on the temperature during refolding, 58% of native activity after refolding at 4°C, overview | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chloroacetyl-L-leucine + H2O | - |
Sus scrofa | chloroacetate + L-leucine | - |
r |