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Literature summary for 3.5.1.14 extracted from

  • Lindner, H.A.; Alary, A.; Wilke, M.; Sulea, T.
    Probing the acyl-binding pocket of aminoacylase-1 (2008), Biochemistry, 47, 4266-4275.
    View publication on PubMed
Search for more literature for 3.5.1.14

Protein Variants

Protein Variants Comment Organism
I177A 2.7fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
I177A 3.5fold increased KM-value compared to the wildtype with N-hexanoyl-L-methionine moiety Homo sapiens
I177L 4fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
I177L 5.2fold increased KM-value compared to the wildtype with N-hexanoyl-L-methionine moiety Homo sapiens
L372A 2fold decreased KM-value compared to the wildtype with N-isopentanoyl-L-methionine Homo sapiens
L372G 10fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
L372I 1,7fold increased KM-value compared to the wildtype with N-isopentanoyl-L-methionine moiety Homo sapiens
L372I 3.1fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
L372V 6.4fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
L372V 7.9fold decreased KM-value compared to the wildtype with N-isopentanoyl-L-methionine moiety Homo sapiens
T347F 1.8fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
T347G 6.5fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
T347S 1.1fold decreased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
T347S 5.3fold decreased KM-value compared to the wildtype with N-cyclohexanoyl-L-methionine moiety Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 N-hexanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
0.03
-
 N-pentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
0.19
-
 N-pentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
0.31
-
 N-cyclohexanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
0.31
-
 N-butanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
0.34
-
 N-propionyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
0.38
-
 N-benzoyl-L-methionine hydrolysis of N-acetyl-L-methionine substrates by Wild-Type porcine Acy1 Sus scrofa
0.4
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant Homo sapiens
0.41
-
 N-benzoyl-L-methionine hydrolysis of N-acetyl-L-methionine substrates by wild-type human Acy1 Homo sapiens
0.43
-
 N-acetyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
0.54
-
 N-isopentanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant Homo sapiens
0.55
-
 N-propionyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
0.64
-
 N-cyclopentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
0.73
-
 N-butanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
0.76
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347F-mutant Homo sapiens
0.81
-
 N-benzoyl-L-methionine hydrolysis of N-acetyl-L-methionine substrates by wild-type human Acy1 Homo sapiens
0.9
-
 N-isopentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
1.01
-
 N-hexanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
1.14
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant Homo sapiens
1.17
-
 N-cyclohexanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant Homo sapiens
1.35
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant Homo sapiens
1.63
-
 N-benzoyl-L-methionine hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1 Homo sapiens
1.72
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant Homo sapiens
2.1
-
 N-isopentanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant Homo sapiens
2.72
-
 N-acetyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
2.76
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant Homo sapiens
2.78
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347G-mutant Homo sapiens
3.56
-
 N-hexanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant Homo sapiens
3.71
-
 N-benzoyl-L-glutamate hydrolysis of N-acetyl-L-glutamate substrates by Wild-Type porcine Acy1 Sus scrofa
4.27
-
 N-isopentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
4.32
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372G-mutant Homo sapiens
5.22
-
 N-hexanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant Homo sapiens
6.23
-
 N-cyclohexanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
7.18
-
 N-cyclopentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
7.29
-
 N-isopentanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant Homo sapiens
15.3
-
 N-isobutanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
16.8
-
 N-formyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
35.4
-
 N-formyl-Lmethionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
58.5
-
 N-isobutanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
63
-
 N-acetyl-L-glutamate hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1 Homo sapiens
70
-
 N-acetyl-L-glutamate hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1 Homo sapiens
102
-
 N-acetyl-L-glutamate hydrolysis of N-acetyl-L-glutamate substrates by Wild-Type porcine Acy1 Sus scrofa
168
-
 N-2,2-dimethylpropionyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
585
-
 N-2,2-dimethylpropionyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa

Organism

Organism UniProt Comment Textmining
Homo sapiens Q03154
-
-
Sus scrofa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-2,2-dimethylpropionyl-L-methionine + H2O
-
 Sus scrofa 2,2-dimethyl-propionate + L-methionine
-
 ?
N-2,2-dimethylpropionyl-L-methionine + H2O
-
 Homo sapiens 2,2-dimethyl-propionate + L-methionine
-
 ?
N-acetyl-L-glutamate + H2O
-
 Sus scrofa acetate + L-glutamate
-
 ?
N-acetyl-L-glutamate + H2O
-
 Homo sapiens acetate + L-glutamate
-
 ?
N-acetyl-L-methionine + H2O
-
 Homo sapiens acetate + L-methionine
-
 ?
N-benzoyl-L-glutamate + H2O
-
 Sus scrofa benzoate + L-glutamate
-
 ?
N-benzoyl-L-glutamate + H2O
-
 Homo sapiens benzoate + L-glutamate
-
 ?
N-benzoyl-L-methionine + H2O
-
 Sus scrofa benzoate + L-methionine
-
 ?
N-benzoyl-L-methionine + H2O
-
 Homo sapiens benzoate + L-methionine
-
 ?
N-butanoyl-L-methionine + H2O
-
 Sus scrofa butanoate + L-methionine
-
 ?
N-butanoyl-L-methionine + H2O
-
 Homo sapiens butanoate + L-methionine
-
 ?
N-cyclohexanoyl-L-methionine + H2O
-
 Homo sapiens hexanoate + L-methionine
-
 ?
N-cyclohexanoyl-L-methionine + H2O
-
 Sus scrofa cyclohexanecarboxylate + L-methionine
-
 ?
N-cyclopentanoyl-L-methionine + H2O
-
 Homo sapiens pentanoate + L-methionine
-
 ?
N-cyclopentanoyl-L-methionine + H2O
-
 Sus scrofa cyclopentanecarboxylate + L-methionine
-
 ?
N-formyl-L-methionine + H2O
-
 Homo sapiens formate + L-methionine
-
 ?
N-formyl-Lmethionine + H2O
-
 Sus scrofa formate + L-methionine
-
 ?
N-hexanoyl-L-methionine + H2O
-
 Sus scrofa hexanoate + L-methionine
-
 ?
N-hexanoyl-L-methionine + H2O
-
 Homo sapiens hexanoate + L-methionine
-
 ?
N-isobutanoyl-L-methionine + H2O
-
 Sus scrofa isobutanoate + L-methionine
-
 ?
N-isobutanoyl-L-methionine + H2O
-
 Homo sapiens isobutanoate + L-methionine
-
 ?
N-isopentanoyl-L-methionine + H2O
-
 Sus scrofa isopentanoate + L-methionine
-
 ?
N-isopentanoyl-L-methionine + H2O
-
 Homo sapiens isopentanoate + L-methionine
-
 ?
N-pentanoyl-L-methionine + H2O
-
 Sus scrofa pentanoate + L-methionine
-
 ?
N-pentanoyl-L-methionine + H2O
-
 Homo sapiens pentanoate + L-methionine
-
 ?
N-propionyl-L-methionine + H2O
-
 Sus scrofa propionate + L-methionine
-
 ?
N-propionyl-L-methionine + H2O
-
 Homo sapiens propionate + L-methionine
-
 ?

Synonyms

Synonyms Comment Organism
hAcy1
-
 Homo sapiens
M20
-
 Sus scrofa
M20
-
 Homo sapiens
pAcy1
-
 Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0015
-
 N-2,2-dimethylpropionyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
0.0015
-
 N-2,2-dimethylpropionyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
0.00623
-
 N-cyclohexanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
0.0101
-
 N-benzoyl-L-glutamate kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1 Homo sapiens
0.052 2.1 N-hexanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant Homo sapiens
0.105
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
0.115
-
 N-benzoyl-L-glutamate kcat for the hydrolysis of N-acetyl-L-glutamate substrates by pAcy1 Sus scrofa
0.129
-
 N-cyclohexanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant Homo sapiens
0.145
-
 N-cyclohexanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
0.158
-
 N-benzoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
0.34
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant Homo sapiens
0.529
-
 N-isobutanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
0.694
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
1.2
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347F-mutant Homo sapiens
1.3
-
 N-benzoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
2.01
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant Homo sapiens
3.3
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant Homo sapiens
4.3
-
 N-hexanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
4.49
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant Homo sapiens
5.36
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant Homo sapiens
6.08
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
6.08
-
 N-isobutanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
9.51
-
 N-hexanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant Homo sapiens
11.2
-
 N-cyclopentanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
12.1
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant Homo sapiens
13.3
-
 N-cyclopentanoyl-L-methionine kcat the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
16.1
-
 N-hexanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
16.2
-
 N-pentanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
16.6
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372G-mutant Homo sapiens
18.2
-
 N-pentanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
20.1
-
 N-benzoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1-T347S-mutant Homo sapiens
25.7
-
 N-propionyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
38.3
-
 N-acetyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
39.4
-
 N-hexanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant Homo sapiens
46
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347G-mutant Homo sapiens
63.4
-
 N-acetyl-L-glutamate kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1-T347S-mutant Homo sapiens
64.6
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant Homo sapiens
66.7
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant Homo sapiens
89.8
-
 N-isobutanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
93.7
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant Homo sapiens
107
-
 N-butanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
120
-
 N-acetyl-L-glutamate kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1-T347S-mutant Homo sapiens
134
-
 N-acetyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
139
-
 N-butanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
156
-
 N-acetyl-L-glutamate kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1 Homo sapiens
168
-
 N-propionyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
177
-
 N-formyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
200
-
 N-formyl-Lmethionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
207
-
 N-acetyl-L-glutamate kcat for the hydrolysis of N-acetyl-L-glutamate substrates by pAcy1 Sus scrofa