Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from kidney by acetone precipitation, ion exchange chromatography, and gel filtration | Sus scrofa |
Renatured (Comment) | Organism |
---|---|
60fold dilution of guanidinum hydrochloride-denatured enzyme, role of osmolytes, e.g. glycerol, DMSO, sucrose, or proline, as chemical chaperones during the refolding of aminoacylase, off-pathway aggregation occurs, osmolytes inhibits aggregation and recover enzyme activity during refolding, overview | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chloroacetyl-L-leucine + H2O | - |
Sus scrofa | chloroacetate + L-leucine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sus scrofa |