Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, subcloning in Escherichia coli strain DH5alpha, expression in Streptomyces lividans strain TK24 resulting in 42fold higher activity compared to the native wild-type Streptomyces mobaraensis strain | Streptomyces mobaraensis |
expressed in Streptomyces lividans strain TK24 | Streptomyces mobaraensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 10% residual activity at 1 mM; 90% inhibition at 1 mM | Streptomyces mobaraensis | |
2-mercaptoethanol | 20% inhibition at 1 mM; 81% residual activity at 1 mM | Streptomyces mobaraensis | |
Ca2+ | 86% residual activity at 0.005 mM | Streptomyces mobaraensis | |
Co2+ | 82% residual activity at 0.005 mM; slight inhibition at 0.5 mM | Streptomyces mobaraensis | |
Cu2+ | 93% residual activity at 0.005 mM; slight inhibition at 0.5 mM | Streptomyces mobaraensis | |
Fe2+ | 86% residual activity at 0.005 mM; 90% inhibition at 0.5 mM | Streptomyces mobaraensis | |
iodoacetamide | 15% inhibition at 1 mM; 85% residual activity at 1 mM | Streptomyces mobaraensis | |
Mg2+ | 96% residual activity at 0.005 mM | Streptomyces mobaraensis | |
Mn2+ | 88% residual activity at 0.005 mM; slight inhibition at 0.5 mM | Streptomyces mobaraensis | |
additional information | the activity of the recombinant enzyme is not influenced by dithiothreitol, reduced glutathione, EDTA, p-chloromercuribenzoic acid, and L-Cys | Streptomyces mobaraensis | |
Ni2+ | slight inhibition at 0.5 mM | Streptomyces mobaraensis | |
Zn2+ | activates 57% at 500 nM, 20% at 0.005 mM, but inhibits 61% at 2.5 mM; slight inhibition at 0.5 mM; the relative remaining activity is decreased to 39% by incubation with 2.5 mM Zn2+ | Streptomyces mobaraensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.3 | - |
N-acetyl-L-Met | recombinant enzyme, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity is not affected by Ca2+ or Mg2+ at 0.5 mM | Streptomyces mobaraensis | |
Ni2+ | 110% relative activity at 0.005 mM | Streptomyces mobaraensis | |
Zn2+ | 120% relative activity at 0.0005 mM | Streptomyces mobaraensis | |
Zn2+ | activates 57% at 500 nM, 20% at 0.005 mM, but inhibits 61% at 2.5 mM | Streptomyces mobaraensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Streptomyces mobaraensis |
55000 | - |
- |
Streptomyces mobaraensis |
55000 | - |
blue native PAGE | Streptomyces mobaraensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptomyces mobaraensis | the enzyme shows high hydrolytic activity towards various N-acetyl-L-amino acids and N-(middle/long)-chain-fatty-acyl-L-amino acids with a preference for the acyl derivatives of L-Met, L-Ala, L-Cys, etc | ? | - |
? | |
N-acetyl-L-alanine + H2O | Streptomyces mobaraensis | - |
acetate + L-alanine | - |
? | |
N-acetyl-L-cysteine + H2O | Streptomyces mobaraensis | - |
acetate + L-cysteine | - |
? | |
N-acetyl-L-methionine + H2O | Streptomyces mobaraensis | - |
acetate + L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces mobaraensis | C9K2Z6 | strain NBRC (IFO) 13819 | - |
Streptomyces mobaraensis | C9K2Z6 | strain NBRC 13819 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, octyl-Sepharose column chromatography, HiTrap chelating column chromatography, and DEAE Sephadex A-50 ion exchange chromatography | Streptomyces mobaraensis |
Streptomyces lividans strain TK24, native enzyme 494fold from by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography | Streptomyces mobaraensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificities of recombinant and native enzyme, overview | Streptomyces mobaraensis |
0.89 | - |
native wild-type enzyme, culture supernatant, substrate N-acetyl-L-methionine, pH 7.5, 37°C | Streptomyces mobaraensis |
0.89 | - |
recombinant wild-type enzyme, culture supernatant, substrate N-acetyl-L-methionine, pH 7.5, 37°C | Streptomyces mobaraensis |
4 | - |
using N-lauroyl-L-Arg as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
6 | - |
using N-lauroyl-L-Asn as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
6 | - |
using N-lauroyl-L-His as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
8 | - |
using N-acetyl-Gly as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
8 | - |
using N-acetyl-L-Asp as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
14 | - |
using N-acetyl-L-Gln as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
14 | - |
using N-lauroyl-L-Lys as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
17 | - |
using N-lauroyl-L-Cys as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
19 | - |
using N-acetyl-L-Phe as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
24 | - |
using N-acetyl-L-Val as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
31 | - |
using N-acetyl-L-Asn as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
34 | - |
using N-lauroyl-Gly as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
36 | - |
using N-lauroyl-L-Ser as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
59 | - |
using N-acetyl-L-His as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
60 | - |
using N-acetyl-L-Leu as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
66 | - |
using N-acetyl-L-Arg as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
72 | - |
using 3 mM N-lauroyl-L-Met as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
89 | - |
using 3 mM N-decanoyl-L-Met as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
96 | - |
purified recombinant wild-type enzyme, substrate N-lauryl-L-alanine, pH 7.5, 37°C | Streptomyces mobaraensis |
96 | - |
using N-lauroyl-L-Ala as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
105 | - |
using 3 mM N-palmitoyl-L-Met as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
106 | - |
using 3 mM N-myristoyl-L-Met as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
169 | - |
using 3 mM N-butyryl-L-Met as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
173 | - |
using 3 mM N-acetyl-L-Met as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
175 | - |
using 3 mM N-hexanoyl-L-Met as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
194 | - |
using N-acetyl-L-Ala as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
251 | - |
using 3 mM N-octanoyl-L-Met as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
412 | - |
purified recombinant wild-type enzyme, substrate N-acetyl-L-cysteine, pH 7.5, 37°C | Streptomyces mobaraensis |
412 | - |
using N-acetyl-L-Cys as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
440 | - |
purified native wild-type enzyme, substrate N-acetyl-L-methionine, pH 7.5, 37°C | Streptomyces mobaraensis |
530 | - |
purified recombinant wild-type enzyme, substrate N-acetyl-L-methionine, pH 7.5, 37°C | Streptomyces mobaraensis |
530 | - |
using 15 mM N-acetyl-L-Met as substrate, at 37°C, pH not specified in the publication | Streptomyces mobaraensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows high hydrolytic activity towards various N-acetyl-L-amino acids and N-(middle/long)-chain-fatty-acyl-L-amino acids with a preference for the acyl derivatives of L-Met, L-Ala, L-Cys, etc | Streptomyces mobaraensis | ? | - |
? | |
additional information | no activity towards N-acetyl-L-Lys, N-acetyl-L-Glu, N-acetyl-L-Pro, N-acetyl-L-Trp, N-acetyl-L-Tyr, N-lauroyl-L-Ile, N-lauroyl-L-Leu, N-lauroyl-L-Phe, N-lauroyl-L-Pro, N-lauroyl-L-Trp, N-lauroyl-L-Tyr, and N-lauroyl-L-Val | Streptomyces mobaraensis | ? | - |
? | |
additional information | the enzyme hydrolyzes N-(middle/long)-chain-fatty-acyl-L-amino acids as well as N-short-chain-acyl-L-amino acids. Poor or no activity with N-acetyl-amino acids containing L-Lys, L-Asp, L-Cys, L-Pro, L-Trp, and L-Tyr, as well as with N-leuroyl-amino acids containing L-Arg, L-His, L-Asn, L-Ile, L-Leu, L-Phe, L-Pro, L-Trp, L-Tyr, and L-Val, substrate specificity, overview | Streptomyces mobaraensis | ? | - |
? | |
N-Acetyl-Gly + H2O | - |
Streptomyces mobaraensis | acetate + Gly | - |
? | |
N-acetyl-L-Ala + H2O | - |
Streptomyces mobaraensis | acetate + L-Ala | - |
? | |
N-acetyl-L-alanine + H2O | - |
Streptomyces mobaraensis | acetate + L-alanine | - |
? | |
N-acetyl-L-Arg + H2O | - |
Streptomyces mobaraensis | acetate + L-Arg | - |
? | |
N-acetyl-L-arginine + H2O | - |
Streptomyces mobaraensis | acetate + L-arginine | - |
? | |
N-acetyl-L-Asn + H2O | - |
Streptomyces mobaraensis | acetate + L-Asn | - |
? | |
N-acetyl-L-Asp + H2O | - |
Streptomyces mobaraensis | acetate + L-Asp | - |
? | |
N-acetyl-L-asparagine + H2O | - |
Streptomyces mobaraensis | acetate + L-asparagine | - |
? | |
N-acetyl-L-Cys + H2O | - |
Streptomyces mobaraensis | acetate + L-Cys | - |
? | |
N-acetyl-L-cysteine + H2O | - |
Streptomyces mobaraensis | acetate + L-cysteine | - |
? | |
N-acetyl-L-Gln + H2O | - |
Streptomyces mobaraensis | acetate + L-Gln | - |
? | |
N-acetyl-L-glutamine + H2O | - |
Streptomyces mobaraensis | acetate + L-glutamine | - |
? | |
N-acetyl-L-histidine + H2O | - |
Streptomyces mobaraensis | acetate + L-histidine | - |
? | |
N-acetyl-L-Leu + H2O | - |
Streptomyces mobaraensis | acetate + L-Leu | - |
? | |
N-acetyl-L-leucine + H2O | - |
Streptomyces mobaraensis | acetate + L-leucine | - |
? | |
N-acetyl-L-Met + H2O | highest activity | Streptomyces mobaraensis | acetate + L-Met | - |
? | |
N-acetyl-L-methionine + H2O | - |
Streptomyces mobaraensis | acetate + L-methionine | - |
? | |
N-acetyl-L-methionine + H2O | preferred substrate | Streptomyces mobaraensis | acetate + L-methionine | - |
? | |
N-acetyl-L-Phe + H2O | - |
Streptomyces mobaraensis | acetate + L-Phe | - |
? | |
N-acetyl-L-phenylalanine + H2O | - |
Streptomyces mobaraensis | acetate + L-phenylalanine | - |
? | |
N-acetyl-L-Val + H2O | - |
Streptomyces mobaraensis | acetate + L-Val | - |
? | |
N-acetyl-L-valine + H2O | - |
Streptomyces mobaraensis | acetate + L-valine | - |
? | |
N-butyryl-L-Met + H2O | - |
Streptomyces mobaraensis | butanoate + L-Met | - |
? | |
N-butyryl-L-methionine + H2O | - |
Streptomyces mobaraensis | butyrate + L-methionine | - |
? | |
N-decanoyl-L-Met + H2O | - |
Streptomyces mobaraensis | decanoate + L-Met | - |
? | |
N-decanoyl-L-methionine + H2O | - |
Streptomyces mobaraensis | decanoate + L-methionine | - |
? | |
N-hexanoyl-L-Met + H2O | - |
Streptomyces mobaraensis | hexanoate + L-Met | - |
? | |
N-hexanoyl-L-methionine + H2O | - |
Streptomyces mobaraensis | hexanoate + L-methionine | - |
? | |
N-lauroyl-Gly + H2O | - |
Streptomyces mobaraensis | laurate + Gly | - |
? | |
N-lauroyl-L-Ala + H2O | - |
Streptomyces mobaraensis | laurate + L-Ala | - |
? | |
N-lauroyl-L-Arg + H2O | - |
Streptomyces mobaraensis | laurate + L-Arg | - |
? | |
N-lauroyl-L-Asn + H2O | - |
Streptomyces mobaraensis | laurate + L-Asn | - |
? | |
N-lauroyl-L-Cys + H2O | - |
Streptomyces mobaraensis | laurate + L-Cys | - |
? | |
N-lauroyl-L-His + H2O | - |
Streptomyces mobaraensis | laurate + L-His | - |
? | |
N-lauroyl-L-Lys + H2O | - |
Streptomyces mobaraensis | laurate + L-Lys | - |
? | |
N-lauroyl-L-Met + H2O | - |
Streptomyces mobaraensis | laurate + L-Met | - |
? | |
N-lauroyl-L-Ser + H2O | - |
Streptomyces mobaraensis | laurate + L-Ser | - |
? | |
N-lauryl-glycine + H2O | - |
Streptomyces mobaraensis | laurate + glycine | - |
? | |
N-lauryl-L-alanine + H2O | - |
Streptomyces mobaraensis | laurate + L-alanine | - |
? | |
N-lauryl-L-cysteine + H2O | - |
Streptomyces mobaraensis | laurate + L-cysteine | - |
? | |
N-lauryl-L-lysine + H2O | - |
Streptomyces mobaraensis | laurate + L-lysine | - |
? | |
N-lauryl-L-methionine + H2O | - |
Streptomyces mobaraensis | laurate + L-methionine | - |
? | |
N-lauryl-L-serine + H2O | - |
Streptomyces mobaraensis | laurate + L-serine | - |
? | |
N-myristoyl-L-Met + H2O | - |
Streptomyces mobaraensis | myristate + L-Met | - |
? | |
N-myristoyl-L-methionine + H2O | - |
Streptomyces mobaraensis | myristate + L-methionine | - |
? | |
N-octanoyl-L-Met + H2O | - |
Streptomyces mobaraensis | octanoate + L-Met | - |
? | |
N-octanoyl-L-methionine + H2O | - |
Streptomyces mobaraensis | octanoate + L-methionine | - |
? | |
N-palmitoyl-L-Met + H2O | - |
Streptomyces mobaraensis | palmitate + L-Met | - |
? | |
N-palmitoyl-L-methionine + H2O | - |
Streptomyces mobaraensis | palmitate + L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000 | Streptomyces mobaraensis |
monomer | 1 * 55000, purified wild type enzyme, SDS-PAGE | Streptomyces mobaraensis |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the peptidase M20 family | Streptomyces mobaraensis |
N-acyl-L-amino-acid amidohydrolase | - |
Streptomyces mobaraensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Streptomyces mobaraensis |
50 | - |
at pH 7.5 | Streptomyces mobaraensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptomyces mobaraensis |