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Literature summary for 3.5.1.14 extracted from

  • Honda, G.; Matsuda, A.; Zushi, M.; Yamamoto, S.; Komatsu, K.
    Heterologous protein production in Acremonium chrysogenum: expression of bacterial cephalosporin C acylase and human thrombomodulin genes (1997), Biosci. Biotechnol. Biochem., 61, 948-955.
    View publication on PubMed
Search for more literature for 3.5.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Acremonium chrysogenum IS-5, transformants with pSACY2 or pMACY2 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 SDS-PAGE Pseudomonas sp.
57000
-
 SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0042
-
 transformant 1 with plasmid pSACY2 Pseudomonas sp.
0.0045
-
 transformant 2 with plasmid pSACY2 Pseudomonas sp.
0.012
-
 transformant 1 with pMACY2 Pseudomonas sp.
0.02
-
 transformant 2 with pMACY2 Pseudomonas sp.
0.025
-
 transformant 3 with pMACY2 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O
-
 Pseudomonas sp. 7-aminocephalosporanic acid + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 26000 + 1 * 57000, SDS-PAGE Pseudomonas sp.