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Literature summary for 3.5.1.14 extracted from
- Comparative studies on soluble and immobilized pig kidney aminoacylase (1980), J. Appl. Biochem., 2, 72-80.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | industrial applications | Sus scrofa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
N-acetyl-L-methionine | immobilized enzyme | Sus scrofa |  |
| 6 | - |
N-acetyl-L-methionine | soluble enzyme | Sus scrofa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-L-methionine | Sus scrofa | - |
L-methionine + acetate | - |
? | |
| N-acetyl-L-methionine | Aspergillus oryzae | - |
L-methionine + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
| Sus scrofa | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-DL-methionine + H2O | - |
Sus scrofa | DL-methionine + acetate | - |
? | |
| N-acetyl-DL-methionine + H2O | - |
Aspergillus oryzae | DL-methionine + acetate | - |
? | |
| N-acetyl-L-alanine + H2O | - |
Sus scrofa | L-alanine + acetate | - |
? | |
| N-acetyl-L-alanine + H2O | - |
Aspergillus oryzae | L-alanine + acetate | - |
? | |
| N-acetyl-L-methionine | - |
Sus scrofa | L-methionine + acetate | - |
? | |
| N-acetyl-L-methionine | - |
Aspergillus oryzae | L-methionine + acetate | - |
? | |
| N-acetyl-L-valine + H2O | - |
Sus scrofa | L-valine + acetate | - |
? | |
| N-acetyl-O-benzyl-DL-serine + H2O | no hydrolysis with immobilized enzyme | Sus scrofa | acetate + O-benzyl-DL-serine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | dissociable enzyme, monomer and dimer forms | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
soluble enzyme | Sus scrofa |
| 65 | - |
immobilized enzyme | Sus scrofa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | - |
Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
immobilized enzyme more thermally stable, preserved during prolonged incubation at 70°C, soluble enzyme completely inactivated in 10 min under same conditions | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Aspergillus oryzae |
| 7 | - |
soluble enzyme | Sus scrofa |
| 7.3 | 7.6 | immobilized enzyme | Sus scrofa |
| 7.5 | - |
immobilized enzyme | Sus scrofa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | stable in 0.1 M potassium phosphate buffer at 55, 60 and 70°C, maximum stability from pH 6.5-6.6, immobilized enzyme at pH 7.2 | Sus scrofa |
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