Application | Comment | Organism |
---|---|---|
synthesis | - |
Sus scrofa |
synthesis | enzyme used for production of L-amino acids | Aspergillus oryzae |
synthesis | thermostable enzyme useful for industrial production, optical resolution of DL-amino acids on an industrial scale, utilization in industrial production of stereoisomers from racemates | Bacillus sp. (in: Bacteria) |
General Stability | Organism |
---|---|
very active and physically stable, resistant to urea, 85% activity retained with 8 M urea at 37°C for 30 min | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Aspergillus oryzae | |
Cd2+ | - |
Bacillus sp. (in: Bacteria) | |
Cu2+ | - |
Aspergillus oryzae | |
Cu2+ | - |
Bacillus sp. (in: Bacteria) | |
Fe2+ | - |
Bacillus sp. (in: Bacteria) | |
Hg2+ | - |
Bacillus sp. (in: Bacteria) | |
Ni2+ | - |
Aspergillus oryzae | |
Ni2+ | - |
Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
N-acetyl-L-methionine | addition of 0.2 mM Co2+ | Bacillus sp. (in: Bacteria) | |
0.056 | - |
N-acetyl-L-methionine | addition of 0.2 mM Mn2+ | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enzyme strongly activated by Co2+ ions | Sus scrofa | |
Co2+ | enzyme strongly activated by Co2+ ions | Bacillus sp. (in: Bacteria) | |
Co2+ | enzyme strongly activated by Co2+ ions | Aspergillus oryzae | |
Mn2+ | - |
Bacillus sp. (in: Bacteria) | |
Zn2+ | enzyme contains Zn as prosthetic metal | Sus scrofa | |
Zn2+ | enzyme contains Zn as prosthetic metal | Bacillus sp. (in: Bacteria) | |
Zn2+ | enzyme contains Zn as prosthetic metal | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
- |
Aspergillus oryzae |
37000 | - |
subunit, SDS-PAGE | Bacillus sp. (in: Bacteria) |
42500 | - |
calculated from amino acid composition | Bacillus sp. (in: Bacteria) |
43000 | - |
2 * 43000, SDS-PAGE | Sus scrofa |
43000 | - |
2 * 43000, SDS-PAGE | Aspergillus oryzae |
43000 | - |
4 * 43000, 4gatom Zn per mol enzyme, SDS-PAGE | Bacillus sp. (in: Bacteria) |
175000 | - |
gel permeation | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-methionine | Sus scrofa | - |
L-methionine + acetate | - |
? | |
N-acetyl-L-methionine | Bacillus sp. (in: Bacteria) | - |
L-methionine + acetate | - |
? | |
N-acetyl-L-methionine | Aspergillus oryzae | - |
L-methionine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Bacillus sp. (in: Bacteria) | - |
thermoglucosidicus, strain DSM 2542 | - |
Sus scrofa | - |
pig | - |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
- |
Bacillus sp. (in: Bacteria) |
- |
Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-DL-methionine + H2O | - |
Sus scrofa | DL-methionine + acetate | - |
? | |
N-acetyl-DL-methionine + H2O | - |
Bacillus sp. (in: Bacteria) | DL-methionine + acetate | - |
? | |
N-acetyl-DL-methionine + H2O | - |
Aspergillus oryzae | DL-methionine + acetate | - |
? | |
N-acetyl-DL-norleucine + H2O | - |
Sus scrofa | DL-norleucine + acetate | - |
? | |
N-acetyl-DL-norleucine + H2O | - |
Bacillus sp. (in: Bacteria) | DL-norleucine + acetate | - |
? | |
N-acetyl-L-alanine + H2O | - |
Sus scrofa | L-alanine + acetate | - |
? | |
N-acetyl-L-alanine + H2O | - |
Bacillus sp. (in: Bacteria) | L-alanine + acetate | - |
? | |
N-acetyl-L-alanine + H2O | - |
Aspergillus oryzae | L-alanine + acetate | - |
? | |
N-acetyl-L-glutamine + H2O | - |
Sus scrofa | L-glutamine + acetate | - |
? | |
N-acetyl-L-glutamine + H2O | - |
Bacillus sp. (in: Bacteria) | L-glutamine + acetate | - |
? | |
N-acetyl-L-glutamine + H2O | - |
Aspergillus oryzae | L-glutamine + acetate | - |
? | |
N-acetyl-L-methionine | - |
Sus scrofa | L-methionine + acetate | - |
? | |
N-acetyl-L-methionine | - |
Bacillus sp. (in: Bacteria) | L-methionine + acetate | - |
? | |
N-acetyl-L-methionine | - |
Aspergillus oryzae | L-methionine + acetate | - |
? | |
N-acetyl-L-phenylalanine + H2O | - |
Sus scrofa | L-phenylalanine + acetate | - |
? | |
N-acetyl-L-phenylalanine + H2O | - |
Bacillus sp. (in: Bacteria) | L-phenylalanine + acetate | - |
? | |
N-acetyl-L-valine + H2O | - |
Sus scrofa | L-valine + acetate | - |
? | |
N-acetyl-L-valine + H2O | - |
Bacillus sp. (in: Bacteria) | L-valine + acetate | - |
? | |
N-chloroacetyl-DL-valine + H2O | - |
Sus scrofa | chloroacetate + DL-valine | - |
? | |
N-chloroacetyl-DL-valine + H2O | - |
Bacillus sp. (in: Bacteria) | chloroacetate + DL-valine | - |
? | |
N-chloroacetyl-L-leucine + H2O | - |
Sus scrofa | chloroacetate + L-leucine | - |
? | |
N-chloroacetyl-L-leucine + H2O | - |
Bacillus sp. (in: Bacteria) | chloroacetate + L-leucine | - |
? | |
N-chloroacetyl-L-leucine + H2O | - |
Aspergillus oryzae | chloroacetate + L-leucine | - |
? | |
N-chloroacetylglycine + H2O | N-chloroacetylamino acids are better substrates than N-acetylamino acids | Bacillus sp. (in: Bacteria) | chloroacetate + glycine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, SDS-PAGE | Sus scrofa |
dimer | 2 * 43000, SDS-PAGE | Aspergillus oryzae |
tetramer | 4 * 43000, 4gatom Zn per mol enzyme, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | retains full activity on heating at 70°C for 10 min, 90% of the original activity after heating at 80%, much activity lost on 30 min at 80°C | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Sus scrofa |
8 | 8.5 | - |
Aspergillus oryzae |
8.3 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11.5 | no activity lost at 37°C for 1h | Bacillus sp. (in: Bacteria) |