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Literature summary for 3.5.1.14 extracted from

  • Hsieh, J.M.; Tsirulnikov, K.; Sawaya, M.R.; Magilnick, N.; Abuladze, N.; Kurtz, I.; Abramson, J.; Pushkin, A.
    Structures of aminoacylase 3 in complex with acetylated substrates (2010), Proc. Natl. Acad. Sci. USA, 107, 17962-17967.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.14

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion technique Mus musculus

Protein Variants

Protein Variants Comment Organism
E167R involved in substrate specificity Mus musculus
E177A catalytically inactive but maintained the structural integrity of active site Mus musculus
E177D reduces the kcat value more than threefold Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
L-benzylsuccinate
-
 Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
StrepTactin Sepharose Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-L-aspartate + H2O no activity of wild type protein, mutant E167R protein shows activity Mus musculus acetate + L-aspartate
-
 ?
N-acetyl-L-tyrosine + H2O
-
 Mus musculus acetate + L-tyrosine
-
 ?
N-acetyl-S-1,2-dichlorovinyl-L-cysteine + H2O
-
 Mus musculus acetate + S-1,2-dichlorovinyl-L-cysteine
-
 ?

Synonyms

Synonyms Comment Organism
aminoacylase 3
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 N-acetyl-L-aspartate E167R mutant protein, pH not specified in the publication, temperature not specified in the publication Mus musculus
0.6
-
 N-acetyl-L-tyrosine E167R mutant protein, pH not specified in the publication, temperature not specified in the publication Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.025
-
 L-benzylsuccinate pH not specified in the publication, temperature not specified in the publication Mus musculus