Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 6% inhibition at 10 mM | Rattus norvegicus | |
DTT | 85% inhibition at 20 mM | Rattus norvegicus | |
EDTA | 10% inhibition at 50 mM | Rattus norvegicus | |
iodoacetamide | 90% inhibition at 10 mM | Rattus norvegicus | |
Nepsilon-formyl-Nepsilon-hydroxy-L-lysine | IC50: 1.0 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
N-acetylmethionine | pH 8.0, 22°C | Rattus norvegicus | |
3.1 | - |
N-formylmethionine | pH 8.0, 22°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | activates 2-3fold at 0.1 mM | Rattus norvegicus | |
NiCl2 | activates 2-3fold at 0.1 mM | Rattus norvegicus | |
Zn2+ | 0.98 Zn2+ per enzyme molecule, tightly bound, involved in cataylsis | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | the enzyme is involved in the degradation of chemotactic N-formyl peptides and plays a protective role in degrading bacterial and mitochondrial N-formylated peptide together with the alpha-N-acylpeptide hydrolase, EC 3.4.19.1 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley rats | - |
Purification (Comment) | Organism |
---|---|
native enzyme from intestinal epithelial mucosa, 1833fold to homogeneity, by acid precipitation, two steps of anion exchange chromatography, hydrophobic interaction chromatography, gel filtration and concanavalin A afinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mucosa | intestinal | Rattus norvegicus | - |
small intestine | mucosa | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
2.2 | - |
purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is involved in the degradation of chemotactic N-formyl peptides and plays a protective role in degrading bacterial and mitochondrial N-formylated peptide together with the alpha-N-acylpeptide hydrolase, EC 3.4.19.1 | Rattus norvegicus | ? | - |
? | |
additional information | no activity with N-formylmethionylalanine, N-formylalanylalanine, N-formylmethionylalanylserine, N-acetylmethionylalanylserine, and N-formylmethionylleucylphenylalanine | Rattus norvegicus | ? | - |
? | |
N-acetylmethionine + H2O | preferred substrate | Rattus norvegicus | acetate + L-methionine | - |
? | |
N-formylmethionine + H2O | substrate synthesis and purification, overview | Rattus norvegicus | formate + L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
fMDF | - |
Rattus norvegicus |
N-acylase IA | - |
Rattus norvegicus |
N-formylmethionine deformylase | - |
Rattus norvegicus |
Nalpha-acyl-L-amino acid amidohydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
N-acetylmethionine | pH 8.0, 22°C | Rattus norvegicus | |
7.9 | - |
N-formylmethionine | pH 8.0, 22°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1 | - |
IC50: 1.0 mM | Rattus norvegicus | Nepsilon-formyl-Nepsilon-hydroxy-L-lysine |