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2.7.2.4: aspartate kinase

This is an abbreviated version!
For detailed information about aspartate kinase, go to the full flat file.

Word Map on EC 2.7.2.4

Reaction

ATP
+
L-aspartate
=
ADP
 +
4-phospho-L-aspartate

Synonyms

AK, AK II, AK III, AK-HSDH, AK-HSDH I, AK-HSDH1, AK-HSDH2, AK-HseDH, AK-R7, AK-ts31d, AK1, AK2, AK3, AKbeta, AKII, AKIII, AKsyn, Ask, ASK1, ASK2, Ask_Ect, Ask_LysC, Asp kinase-homoserine dehydrogenase, aspartate kinase, aspartate kinase (phosphorylating), aspartate kinase 1, aspartate kinase 2, aspartate kinase 3, aspartate kinase beta, aspartate kinase I, aspartate kinase II, aspartate kinase III, aspartic kinase, aspartokinase, aspartokinase 1-homoserine dehydrogenase 1, aspartokinase 2, aspartokinase 3, aspartokinase I, aspartokinase II, aspartokinase III, aspartokinase-homoserine dehydrogenase, beta-aspartokinase, bifunctional aspartate kinase-homoserine dehydrogenase, BT, BTT, CgAK, dap-aspartokinase, HOM3 product, HOM3-R7 product, HOM3-ts31d product, HseDH, LT-aspartokinase, lysC, lysine-sensitive aspartokinase 3, More, MtbAKbeta, nspJ, Thr-sensitive aspartate kinase, thrA, thrA1, thrA2, TM_0547, XbAK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.4 aspartate kinase

Molecular Weight

Molecular Weight on EC 2.7.2.4 - aspartate kinase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
110000
113000
-
gel filtration, native PAGE, isoenzyme AK Late
115000
-
aspartokinase II, equilibrium sedimentation
116000
-
equilibrium ultracentrifugation
121400
122000
-
2 * 122000, ultracentrifugation in TES or HEPES buffer
122400
-
alpha2, beta2, calculated from nucleotide sequence
124400
-
alpha2, beta2, calculated from nucleotide sequence
125000
-
aspartokinase II
126000
-
gel filtration
127000
-
aspartokinase III, sedimentation equilibrium
133000
-
sedimentation velocity centrifugation
137000
-
gel filtration
140000
gel filtration, homodimer
150000
-
sucrose density gradient centrifugation, peak 2
154000
dynamic light-scattering, crystallization
160000
-
gel filtration
166000
-
in presence of KCl and L-lysine at 11°C, sedimentation equilibrium
167000
-
first AK isoenzyme, gel filtration
169000
-
aspartokinase II, equilibrium sedimentation
17000
17700
aspartate kinase beta, calculated from amino acid sequence
17720
18000
180000 - 200000
-
aspartokinase devoid of homoserine dehydrogenase activity in presence of threonine, gel filtration
181000
-
in presence of KCl and L-lysine at 25°C, sedimentation equilibrium
18145
-
1 * 44108 + 1 * 18145, ask alpha and ask beta, SDS-PAGE
18500
-
beta subunit, calculated from nucleotide sequence
200000
-
isoenzyme II, gel filtration
20200
AKbeta analytical ultracentrifugation, absence of threonine
21700
aspartate kinase beta, gel filtration
23000
230000
-
sucrose density gradient centrifugation, peak 3
240000
-
calculated from Stokes' radius
242000
-
gel filtration
246000
-
gel filtration, Superose 6
250000
-
aspartokinase I
253000
-
gel filtration, non-denaturing electrophoresis, 4-20% polyacrylamide gradient gels
254000
-
gel filtration
255000
-
gel filtration
258000
-
gel filtration, Superose 12
280000
297000
-
glutathione-S-transferase fusion protein, in the presence of threonine, blue native gel electrophoresis
298000
-
wild type, in the presence of threonine, gel filtration
320000
gel filtration, in presence of 5.0 mM L-threonine
330000
-
isoenzyme I, gel filtration
33300
AKbeta gel-filtration chromatography, addition of threonine induces dimerization
334000
-
native complex, Svedberg equation
33800
AKbeta in presence with 5 mM L-threonine, gel filtration
344000
345000
346000
358000
-
light scattering studies
36000
AKbeta analytical ultracentrifugation, presence of threonine
360000
-
equilibrium sedimentation
40000
-
6 * 40000, SDS-PAGE
42000
-
SDS-PAGE, recombinant protein
42700
43000
43200
-
alpha subunit, calculated from nucleotide sequence
43700
-
alpha subunit, calculated from nucleotide sequence
43900
44000
-
SDS-PAGE, recombinant protein
44108
-
1 * 44108 + 1 * 18145, ask alpha and ask beta, SDS-PAGE
44300
-
calculated from cDNA
45000
-
1 * 18000 + 1 * 45000, Western blot immunoanalysis
450000
47000
470000
48000
-
4 * 48000, SDS-PAGE
49000
-
2 * 49000 + 2 * 60000, SDS-PAGE
50000
52400
-
calculated from cDNA
53000
-
1 * 17000 + 1 * 53000, urea treatment, SDS-PAGE
53200
55100
55900
58000
58100
-
wild type, calculated from protein sequence
58700
-
wild type, SDS-PAGE
60000
60700
61200
-
alphabeta, calculated from nucleotide sequence
62200
-
alphabeta, calculated from nucleotide sequence
64200
66000
-
4 * 66000, ultracentrifugation
70000
-
gel filtration
79000
-
second AK isoenzyme, gel filtration
80000
-
4 * 80000, aspartokinase-homoserine dehydrogenase complex, sedimentation equlibrium performed on guanidinium chloride dissolved complex
83000
dynamic light-scattering, addition of increasing levels of guanidine-HCl up to 2000 mM causes a decrease in the AK particle size, dissociation into dimers
84000
-
4 * 84000, SDS-PAGE
87500
-
4 * 87500, SDS-PAGE
87800
88000
-
4 * 88000, gel filtration in 6.0 mM guanidinium chloride
90300
-
alpha2, beta2, gel filtration
93000
? * 93000, SDS-PAGE
95000
-
aspartokinase II