Information on EC 2.7.2.4 - aspartate kinase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
2.7.2.4
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RECOMMENDED NAME
GeneOntology No.
aspartate kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-aspartate = ADP + 4-phospho-L-aspartate
show the reaction diagram
; The enzyme from E. coli is a multifunctional protein, which also catalyses the reaction of EC 1.1.1.3 homoserine dehydrogenase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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Biosynthesis of secondary metabolites
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Cysteine and methionine metabolism
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ectoine biosynthesis
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Glycine, serine and threonine metabolism
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grixazone biosynthesis
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L-homoserine biosynthesis
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L-lysine biosynthesis I
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L-lysine biosynthesis II
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L-lysine biosynthesis III
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L-lysine biosynthesis VI
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Lysine biosynthesis
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Metabolic pathways
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Microbial metabolism in diverse environments
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Monobactam biosynthesis
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norspermidine biosynthesis
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spermidine biosynthesis II
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threonine metabolism
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SYSTEMATIC NAME
IUBMB Comments
ATP:L-aspartate 4-phosphotransferase
The enzyme from Escherichia coli is a multifunctional protein, which also catalyses the reaction of EC 1.1.1.3 homoserine dehydrogenase. This is also the case for two of the four isoenzymes in Arabidopsis thaliana. The equilibrium constant strongly favours the reaction from right to left, i.e. the non-physiological direction of reaction.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-50-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
gene lysC
UniProt
Manually annotated by BRENDA team
VB217, ATCC6051
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Manually annotated by BRENDA team
strain HD100
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Manually annotated by BRENDA team
strain HD100
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Manually annotated by BRENDA team
strain I110proC
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Manually annotated by BRENDA team
strain I110proC
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-
Manually annotated by BRENDA team
ATCC 10068
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
carrot
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Manually annotated by BRENDA team
9723 (ATCC)
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Manually annotated by BRENDA team
K12
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Manually annotated by BRENDA team
strain NCIMB 8826
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Manually annotated by BRENDA team
strain NCIMB 8826
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain MCS10
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Manually annotated by BRENDA team
strain MCS10
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Manually annotated by BRENDA team
strain AV19
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
homozygous tobacco plants expressing bacterialAK are crossed with homozygous transgenic tobacco lines expressing F-AtCGS, T-AtCGS (lacking its N-terminus region) or D-AtCGS (lacking 90-nt of the N-terminus region)
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Manually annotated by BRENDA team
no activity in Edwardsiella sp.
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Manually annotated by BRENDA team
no activity in Providencia sp.
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Manually annotated by BRENDA team
no activity in Streptomyces akiyoshiensis
producer strain of 5-hydroxy-4-oxonorvaline
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Manually annotated by BRENDA team
rice, IAC-165 upland variety
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Manually annotated by BRENDA team
strain 63 (ATCC 25901)
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain HT1(pIVUts31d), has a feedback-resistent aspartate kinase
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Manually annotated by BRENDA team
strain SG211, contains HOM3-R7 allel, insensitive to threonine
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Manually annotated by BRENDA team
Massa 03
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Manually annotated by BRENDA team
strain BHT
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Manually annotated by BRENDA team
strain BHT
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
wheat
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Manually annotated by BRENDA team
transgenic narbon bean
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + DL-threo-3-methyl aspartate
ADP + 3-methyl-4-phosphoaspartate
show the reaction diagram
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aspartokinase III
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-
?
ATP + L-asparagine
?
show the reaction diagram
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aspartokinase III
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-
?
ATP + L-aspartate
ADP + 4-phospho-L-aspartate
show the reaction diagram
ATP + L-aspartate
ADP + phospho-L-aspartate
show the reaction diagram
ATP + L-aspartate beta-hydroxamate
?
show the reaction diagram
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aspartokinase III
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-
?
ATP + L-aspartate beta-methyl ester
?
show the reaction diagram
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aspartokinase III
-
-
?
ATP + L-aspartic acid 1-benzyl ester
?
show the reaction diagram
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aspartokinase III
-
-
?
ATP + L-aspartic acid 4-benzyl ester
?
show the reaction diagram
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aspartokinase III
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-
?
ATP + L-aspartic acid amide
ADP + 4-phospho-L-aspartic acid amide
show the reaction diagram
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aspartokinase III
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?
ATP + N-acetyl-L-aspartate
ADP + N-acetyl-4-phospho-L-aspartate
show the reaction diagram
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aspartokinase III
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?
ATP + N-chloroacetyl-L-aspartate
ADP + N-chloroacetyl-4-phospho-L-aspartate
show the reaction diagram
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aspartokinase III
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?
ATP + N-formyl-L-aspartate
ADP + N-formyl-4-phospho-L-aspartate
show the reaction diagram
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aspartokinase III
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?
GTP + L-aspartate
GDP + 4-phospho-L-aspartate
show the reaction diagram
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-
r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-aspartate
ADP + 4-phospho-L-aspartate
show the reaction diagram
ATP + L-aspartate
ADP + phospho-L-aspartate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-hydroxy-L-norvaline
works as L-threonine analog
ADP
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competitive inhibition
aspartate-beta-semialdehyde
D-aspartate beta-hydroxamate
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D-aspartic acid 1-benzyl ester
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Diethyl aminomalonate
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diethyldicarbonate
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DL-2,6-Diaminoheptanoate
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DL-3-amino-4-hydroxybutyrate methyl ester
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DL-3-aminobutyrate
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DL-3-hydroxy-L-norvaline
L-lysine-analog
DL-meso-diaminopimelic acid
HgSO4
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15% inhibition
iodosalicylate
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20% inhibition
L-2-aminobutyrate
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competitive inhibitor
L-alanine
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no significant effect on AK activity at 5 and 20 mM
L-glutamate
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L-glutamate gamma-methyl ester
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L-homoserine
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L-isoleucine
L-leucine
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allosteric effector
L-lysine
L-Lysine ethyl ester
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L-malate
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L-methionine
L-norvaline
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L-threonine
L-Threonine methyl ester
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in combination with either L-lysine or L-methionine
malonate
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methionine
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concerted feedback inhibition with L-threonine
N-Acetylimidazole
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N-epsilon-formyl-L-lysine
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N-ethylmaleimide
p-chloromercuribenzoate
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Rose bengal
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S-(2-aminoethyl)-L-cysteine
L-threonine-analog; works as L-lysine analog
S-2-aminoethyl-L-cysteine
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less effective on the first isoenzyme than L-lysine alone
S-adenosyl-L-methionine
succinate
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Tetranitromethane
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additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-Alanine
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10% activation at 0.33 M
Calmodulin
L-alanine
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13fold activation at 2.5 mM; 3fold activation at 2.5 mM
L-cysteine
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13fold activation at 2.5 mM; 3fold activation at 2.5 mM
L-isoleucine
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13fold activation at 2.5 mM; 3fold activation at 2.5 mM
L-leucine
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4fold activation at 2.5 mM
L-lysine
L-methionine
L-serine
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13fold activation at 2.5 mM; 3fold activation at 2.5 mM
L-threonine
L-valine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.095 - 2.037
aspartate
0.18 - 23.5
ATP
147
DL-threo-3-methyl aspartate
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pH 8.0, 25C, aspartokinase III
16
L-asparagine
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pH 8.0, 25C, aspartokinase III
0.19 - 50.1
L-aspartate
2.5
L-aspartate beta-benzyl ester
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pH 8.0, 25C, aspartokinase III
13
L-aspartate beta-hydroxamate
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pH 8.0, 25C, aspartokinase III
4.9
L-aspartate beta-methyl ester
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pH 8.0, 25C, aspartokinase III
1.8 - 50.1
L-aspartic acid
5.3
L-aspartic acid 1-benzyl ester
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pH 8.0, 25C, aspartokinase III
184
L-aspartic acid amide
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pH 8.0, 25C, aspartokinase III
48
N-acetyl-L-aspartate
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pH 8.0, 25C, aspartokinase III
68
N-Chloroacetyl-L-aspartate
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pH 8.0, 25C, aspartokinase III
41
N-formyl-L-aspartate
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pH 8.0, 25C, aspartokinase III
additional information
additional information
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.333
aspartate
Bacillus subtilis
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pH 7.0, 30C
0.31 - 56.9
ATP
0.16 - 245
L-aspartate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
110 - 370
ATP
4
44 - 290
L-aspartate
97
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
87
D-aspartate beta-hydroxamate
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pH 8.0, 25C, aspartokinase III
14
D-aspartic acid 1-benzyl ester
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pH 8.0, 25C, aspartokinase III
63
Diethyl aminomalonate
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pH 8.0, 25C, aspartokinase III
14
DL-3-amino-4-hydroxybutyrate methyl ester
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pH 8.0, 25C, aspartokinase III
245
DL-3-aminobutyrate
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pH 8.0, 25C, aspartokinase III
28
L-2-aminobutyrate
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pH 8.0, 25C, aspartokinase III
128
L-glutamate
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pH 8.0, 25C, aspartokinase III
82
L-glutamate gamma-methyl ester
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pH 8.0, 25C, aspartokinase III
7.7
L-homoserine
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pH 8.0, 25C, aspartokinase III
1.1
L-leucine
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-
0.1 - 10
L-lysine
53
L-malate
-
pH 8.0, 25C, aspartokinase III
32
L-norvaline
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pH 8.0, 25C, aspartokinase III
0.049 - 28
L-threonine
108
malonate
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pH 8.0, 25C, aspartokinase III
159
succinate
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pH 8.0, 25C, aspartokinase III
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08 - 157.2
L-lysine
0.3 - 18.7
L-threonine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00096
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thrA2, in the presence of L-lysine
0.0013
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mutant S2207A
0.0064
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wild-type
0.0068
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mutant RL4
0.0107
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complemented mutant A2207A/pM1-1
0.0268
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wild type X-2180-1A
0.08
at 37C culture temperature and 22C assay temperature
0.09
at 22C culture temperature and 22C assay temperature
0.11
in the presence of 20 mM threonine
0.16
at 37C culture temperature and 22C assay temperature
0.196
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thrA2, in the presence of L-threonine
0.2
at 22C culture temperature and 22C assay temperature
0.21
at 37C culture temperature and 37C assay temperature
0.26
at 22C culture temperature and 22C assay temperature
0.27
at 22C culture temperature and 37C assay temperature
0.289
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thrA2, in the presence of L-isoleucine
0.29
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thrA2, in the presence of L-methionine
0.36
at 37C culture temperature and 37C assay temperature
0.38
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truncated protein, which contains only the N-terminal region
0.39
at 22C culture temperature and 37C assay temperature
0.4
at 22C culture temperature and 37C assay temperature
0.488
in the presence of 20 mM threonine
0.51
at 37C culture temperature and 22C assay temperature
1.12
at 37C culture temperature and 37C assay temperature
5.6
glutathione-S-transferase fusion protein, 22C assay temperature
5.9
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wild type