Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-alanine | 13fold activation at 2.5 mM | Arabidopsis thaliana | |
L-alanine | 3fold activation at 2.5 mM | Arabidopsis thaliana | |
L-cysteine | 13fold activation at 2.5 mM | Arabidopsis thaliana | |
L-cysteine | 3fold activation at 2.5 mM | Arabidopsis thaliana | |
L-isoleucine | 13fold activation at 2.5 mM | Arabidopsis thaliana | |
L-isoleucine | 3fold activation at 2.5 mM | Arabidopsis thaliana | |
L-leucine | 4fold activation at 2.5 mM | Arabidopsis thaliana | |
L-serine | 13fold activation at 2.5 mM | Arabidopsis thaliana | |
L-serine | 3fold activation at 2.5 mM | Arabidopsis thaliana | |
L-valine | 13fold activation at 2.5 mM | Arabidopsis thaliana | |
L-valine | 3fold activation at 2.5 mM | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
General Stability | Organism |
---|---|
pure protein , quickly frozen in N2 can be stored at -80°C for several months without any loss of activity | Arabidopsis thaliana |
pure protein, quickly frozen in N2 can be stored at -80°C for several months without any loss of activity | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-leucine | allosteric effector | Arabidopsis thaliana | |
L-threonine | in the presence of 1 mM aspartate and 2 mM ATP | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
ATP | 50 mM aspartate, presence of alanine | Arabidopsis thaliana | |
0.48 | - |
ATP | 50 mM aspartate, presence of alanine | Arabidopsis thaliana | |
1.5 | - |
L-aspartate | 20 mM ATP, presence of alanine | Arabidopsis thaliana | |
2.2 | - |
ATP | 50 mM aspartate, absence of alanine | Arabidopsis thaliana | |
2.3 | - |
L-aspartate | 20 mM ATP, presence of alanine | Arabidopsis thaliana | |
2.6 | - |
L-aspartate | 20 mM ATP, absence of alanine | Arabidopsis thaliana | |
6.15 | - |
L-aspartate | 20 mM ATP, absence of alanine | Arabidopsis thaliana | |
6.5 | - |
ATP | 50 mM aspartate, absence of alanine | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | Arabidopsis thaliana | - |
ADP + 4-phospho-L-aspartate | - |
? | |
ATP + L-aspartate | Arabidopsis thaliana | - |
ADP + phospho-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Arabidopsis thaliana | ADP + 4-phospho-L-aspartate | - |
? | |
ATP + L-aspartate | - |
Arabidopsis thaliana | ADP + phospho-L-aspartate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AK-HSDH I | - |
Arabidopsis thaliana |
aspartate kinase I | also acts as homoserine dehydrogenase (EC 1.1.1.3) | Arabidopsis thaliana |
aspartate kinase II | also acts as homoserine dehydrogenase (EC 1.1.1.3) | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
L-threonine | in the presence of 1 mM aspartate and 2 mM ATP | Arabidopsis thaliana | |
0.091 | - |
L-threonine | in the presence of 1 mM aspartate and 2 mM ATP | Arabidopsis thaliana | |
1.1 | - |
L-leucine | - |
Arabidopsis thaliana |