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Literature summary for 2.7.2.4 extracted from
- Catalytic and regulatory properties of meso-diaminopimelate-sensitive aspartokinase from Bacillus stearothermophilus (1971), Arch. Biochem. Biophys., 147, 683-691.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | significantly stimulates dap-aspartokinase | Geobacillus stearothermophilus |  |
General Stability
| General Stability | Organism |
|---|---|
| feedback inhibitor can partially protect from heat inactivation up to 70°C | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DL-meso-diaminopimelic acid | - |
Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.6 | - |
L-aspartate | pH 8.0, 55°C, in presence of inhibitor | Geobacillus stearothermophilus | |
| 5 | - |
L-aspartate | pH 8.0, 55°C | Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | significantly stimulates dap-aspartokinase | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
- |
Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate | Geobacillus stearothermophilus | - |
ADP + 4-phospho-L-aspartate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.046 | - |
- |
Geobacillus stearothermophilus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, storage of the enzyme in the frozen state followed by subsequent thawing leads to a rapid decline in activity | Geobacillus stearothermophilus |
| 4°C, enzyme activity can be maintained for several months | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate | - |
Geobacillus stearothermophilus | ADP + 4-phospho-L-aspartate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dap-aspartokinase | mesodiaminopimelate-sensitive isoenzyme | Geobacillus stearothermophilus |
| LT-aspartokinase | lysine-threonine-sensitive isoenzyme | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
very rapid loss of catalytic activity above | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 7.1 | meso-diaminopimelate sensitive isoenzyme | Geobacillus stearothermophilus |
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