Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D34E | wild type background | Saccharomyces cerevisiae |
E282D | feedback-resistant | Saccharomyces cerevisiae |
S399F | feedback-resistant | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-threonine | 75% inhibition at 10 mM, 22°C culture temperature, 22°C assay temperature, 71% inhibition at 10 mM, 37°C culture temperature, 22°C assay temperature, 87% inhibition at 10 mM, 22°C culture temperature, 37°C assay temperature, 86% inhibition at 10 mM, 37°C culture temperature, 37°C assay temperature, 93% inhibition of glutathione-S-transferase fusion protein at 10 mM, 22°C assay temperature, 73% inhibition of glutathione-S-transferase fusion protein at 10 mM, 37°C assay temperature; reduced sensitivity to threonine inhibition, 0% inhibition at 10 mM, 22°C culture temperature, 22°C assay temperature, 15% inhibition at 10 mM, 37°C culture temperature, 22°C assay temperature, 4% inhibition at 10 mM, 22°C culture temperature, 37°C assay temperature, 14% inhibition at 10 mM, 37°C culture temperature, 37°C assay temperature; resistance to feedback inhibition, 16% inhibition at 10 mM, 22°C culture temperature, 22°C assay temperature, 15% inhibition at 10 mM, 37°C culture temperature, 22°C assay temperature, 9% inhibition at 10 mM, 22°C culture temperature, 37°C assay temperature, 6% inhibition at 10 mM, 37°C culture temperature, 37°C assay temperature, 31% inhibition of GST-fusion protein at 10 mM, 22°C assay temperature, 16% inhibition of glutathione-S-transferase fusion protein at 10 mM, 37°C assay temperature | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | Saccharomyces cerevisiae | - |
ADP + phospho-L-aspartate | - |
? | |
ATP + L-aspartate | Saccharomyces cerevisiae HT1(pIVUts31d) | - |
ADP + phospho-L-aspartate | - |
? | |
ATP + L-aspartate | Saccharomyces cerevisiae SG211 | - |
ADP + phospho-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
feedback-resistent aspartate kinase | - |
Saccharomyces cerevisiae | - |
contains HOM3-R7 allel, insensitive to threonine | - |
Saccharomyces cerevisiae | P10869 | wildtype strain 8723c, parental strain of SG211 | - |
Saccharomyces cerevisiae HT1(pIVUts31d) | - |
feedback-resistent aspartate kinase | - |
Saccharomyces cerevisiae SG211 | - |
contains HOM3-R7 allel, insensitive to threonine | - |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
at 37°C culture temperature and 22°C assay temperature | Saccharomyces cerevisiae |
0.09 | - |
at 22°C culture temperature and 22°C assay temperature | Saccharomyces cerevisiae |
0.11 | - |
in the presence of 20 mM threonine | Saccharomyces cerevisiae |
0.16 | - |
at 37°C culture temperature and 22°C assay temperature | Saccharomyces cerevisiae |
0.2 | - |
at 22°C culture temperature and 22°C assay temperature | Saccharomyces cerevisiae |
0.21 | - |
at 37°C culture temperature and 37°C assay temperature | Saccharomyces cerevisiae |
0.26 | - |
at 22°C culture temperature and 22°C assay temperature | Saccharomyces cerevisiae |
0.27 | - |
at 22°C culture temperature and 37°C assay temperature | Saccharomyces cerevisiae |
0.36 | - |
at 37°C culture temperature and 37°C assay temperature | Saccharomyces cerevisiae |
0.39 | - |
at 22°C culture temperature and 37°C assay temperature | Saccharomyces cerevisiae |
0.4 | - |
at 22°C culture temperature and 37°C assay temperature | Saccharomyces cerevisiae |
0.488 | - |
in the presence of 20 mM threonine | Saccharomyces cerevisiae |
0.51 | - |
at 37°C culture temperature and 22°C assay temperature | Saccharomyces cerevisiae |
0.698 | - |
- |
Saccharomyces cerevisiae |
1.12 | - |
at 37°C culture temperature and 37°C assay temperature | Saccharomyces cerevisiae |
1.373 | - |
- |
Saccharomyces cerevisiae |
5.6 | - |
glutathione-S-transferase fusion protein, 22°C assay temperature | Saccharomyces cerevisiae |
10.6 | - |
glutathione-S-transferase fusion protein, 37°C assay temperature | Saccharomyces cerevisiae |
45.6 | - |
GST-fusion protein, 37°C assay temperature | Saccharomyces cerevisiae |
52.2 | - |
GST-fusion protein, 22°C assay temperature | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Saccharomyces cerevisiae | ADP + phospho-L-aspartate | - |
? | |
ATP + L-aspartate | - |
Saccharomyces cerevisiae HT1(pIVUts31d) | ADP + phospho-L-aspartate | - |
? | |
ATP + L-aspartate | - |
Saccharomyces cerevisiae SG211 | ADP + phospho-L-aspartate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AK | - |
Saccharomyces cerevisiae |
AK-R7 | - |
Saccharomyces cerevisiae |
AK-ts31d | - |
Saccharomyces cerevisiae |
aspartate kinase | - |
Saccharomyces cerevisiae |
HOM3 product | - |
Saccharomyces cerevisiae |
HOM3-R7 product | - |
Saccharomyces cerevisiae |
HOM3-ts31d product | - |
Saccharomyces cerevisiae |