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Literature summary for 2.7.2.4 extracted from

  • Velasco, I.; Arevalo-Rodriguez, M.; Marina, P.; Calderon, I.L.
    A new mutation in the yeast aspartate kinase induces threonine accumulation in a temperature-regulated way (2005), Yeast, 22, 99-110.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D34E wild type background Saccharomyces cerevisiae
E282D feedback-resistant Saccharomyces cerevisiae
S399F feedback-resistant Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
L-threonine 75% inhibition at 10 mM, 22°C culture temperature, 22°C assay temperature, 71% inhibition at 10 mM, 37°C culture temperature, 22°C assay temperature, 87% inhibition at 10 mM, 22°C culture temperature, 37°C assay temperature, 86% inhibition at 10 mM, 37°C culture temperature, 37°C assay temperature, 93% inhibition of glutathione-S-transferase fusion protein at 10 mM, 22°C assay temperature, 73% inhibition of glutathione-S-transferase fusion protein at 10 mM, 37°C assay temperature; reduced sensitivity to threonine inhibition, 0% inhibition at 10 mM, 22°C culture temperature, 22°C assay temperature, 15% inhibition at 10 mM, 37°C culture temperature, 22°C assay temperature, 4% inhibition at 10 mM, 22°C culture temperature, 37°C assay temperature, 14% inhibition at 10 mM, 37°C culture temperature, 37°C assay temperature; resistance to feedback inhibition, 16% inhibition at 10 mM, 22°C culture temperature, 22°C assay temperature, 15% inhibition at 10 mM, 37°C culture temperature, 22°C assay temperature, 9% inhibition at 10 mM, 22°C culture temperature, 37°C assay temperature, 6% inhibition at 10 mM, 37°C culture temperature, 37°C assay temperature, 31% inhibition of GST-fusion protein at 10 mM, 22°C assay temperature, 16% inhibition of glutathione-S-transferase fusion protein at 10 mM, 37°C assay temperature Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate Saccharomyces cerevisiae
-
ADP + phospho-L-aspartate
-
?
ATP + L-aspartate Saccharomyces cerevisiae HT1(pIVUts31d)
-
ADP + phospho-L-aspartate
-
?
ATP + L-aspartate Saccharomyces cerevisiae SG211
-
ADP + phospho-L-aspartate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
feedback-resistent aspartate kinase
-
Saccharomyces cerevisiae
-
contains HOM3-R7 allel, insensitive to threonine
-
Saccharomyces cerevisiae P10869 wildtype strain 8723c, parental strain of SG211
-
Saccharomyces cerevisiae HT1(pIVUts31d)
-
feedback-resistent aspartate kinase
-
Saccharomyces cerevisiae SG211
-
contains HOM3-R7 allel, insensitive to threonine
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
at 37°C culture temperature and 22°C assay temperature Saccharomyces cerevisiae
0.09
-
at 22°C culture temperature and 22°C assay temperature Saccharomyces cerevisiae
0.11
-
in the presence of 20 mM threonine Saccharomyces cerevisiae
0.16
-
at 37°C culture temperature and 22°C assay temperature Saccharomyces cerevisiae
0.2
-
at 22°C culture temperature and 22°C assay temperature Saccharomyces cerevisiae
0.21
-
at 37°C culture temperature and 37°C assay temperature Saccharomyces cerevisiae
0.26
-
at 22°C culture temperature and 22°C assay temperature Saccharomyces cerevisiae
0.27
-
at 22°C culture temperature and 37°C assay temperature Saccharomyces cerevisiae
0.36
-
at 37°C culture temperature and 37°C assay temperature Saccharomyces cerevisiae
0.39
-
at 22°C culture temperature and 37°C assay temperature Saccharomyces cerevisiae
0.4
-
at 22°C culture temperature and 37°C assay temperature Saccharomyces cerevisiae
0.488
-
in the presence of 20 mM threonine Saccharomyces cerevisiae
0.51
-
at 37°C culture temperature and 22°C assay temperature Saccharomyces cerevisiae
0.698
-
-
Saccharomyces cerevisiae
1.12
-
at 37°C culture temperature and 37°C assay temperature Saccharomyces cerevisiae
1.373
-
-
Saccharomyces cerevisiae
5.6
-
glutathione-S-transferase fusion protein, 22°C assay temperature Saccharomyces cerevisiae
10.6
-
glutathione-S-transferase fusion protein, 37°C assay temperature Saccharomyces cerevisiae
45.6
-
GST-fusion protein, 37°C assay temperature Saccharomyces cerevisiae
52.2
-
GST-fusion protein, 22°C assay temperature Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate
-
Saccharomyces cerevisiae ADP + phospho-L-aspartate
-
?
ATP + L-aspartate
-
Saccharomyces cerevisiae HT1(pIVUts31d) ADP + phospho-L-aspartate
-
?
ATP + L-aspartate
-
Saccharomyces cerevisiae SG211 ADP + phospho-L-aspartate
-
?

Synonyms

Synonyms Comment Organism
AK
-
Saccharomyces cerevisiae
AK-R7
-
Saccharomyces cerevisiae
AK-ts31d
-
Saccharomyces cerevisiae
aspartate kinase
-
Saccharomyces cerevisiae
HOM3 product
-
Saccharomyces cerevisiae
HOM3-R7 product
-
Saccharomyces cerevisiae
HOM3-ts31d product
-
Saccharomyces cerevisiae