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2.7.2.4: aspartate kinase

This is an abbreviated version!
For detailed information about aspartate kinase, go to the full flat file.

Word Map on EC 2.7.2.4

Reaction

ATP
+
L-aspartate
=
ADP
 +
4-phospho-L-aspartate

Synonyms

AK, AK II, AK III, AK-HSDH, AK-HSDH I, AK-HSDH1, AK-HSDH2, AK-HseDH, AK-R7, AK-ts31d, AK1, AK2, AK3, AKbeta, AKII, AKIII, AKsyn, Ask, ASK1, ASK2, Ask_Ect, Ask_LysC, Asp kinase-homoserine dehydrogenase, aspartate kinase, aspartate kinase (phosphorylating), aspartate kinase 1, aspartate kinase 2, aspartate kinase 3, aspartate kinase beta, aspartate kinase I, aspartate kinase II, aspartate kinase III, aspartic kinase, aspartokinase, aspartokinase 1-homoserine dehydrogenase 1, aspartokinase 2, aspartokinase 3, aspartokinase I, aspartokinase II, aspartokinase III, aspartokinase-homoserine dehydrogenase, beta-aspartokinase, bifunctional aspartate kinase-homoserine dehydrogenase, BT, BTT, CgAK, dap-aspartokinase, HOM3 product, HOM3-R7 product, HOM3-ts31d product, HseDH, LT-aspartokinase, lysC, lysine-sensitive aspartokinase 3, More, MtbAKbeta, nspJ, Thr-sensitive aspartate kinase, thrA, thrA1, thrA2, TM_0547, XbAK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.4 aspartate kinase

Storage Stability

Storage Stability on EC 2.7.2.4 - aspartate kinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, can be stored over a period of 6 months with a 40% loss of activity, longer storage does not lead to further inactivation
-
-15°C, aspartokinase II, stable in buffer containing 20% glycerol, remaining 100% active and homogenous for several months
-
-15°C, diluted with glycerol to a final concentration of 40%, no loss of enzyme activity observed over a period of more than 1 year
-
-15°C, dissolved in 0.05 M TES buffer containing 30% glycerol, pH 8.0, activity remains unchanged after 2.5 months
-
-20°C, appears to be stable over a period of several months' storage
-
-20°C, can be stored without any noticeable loss in activity
-
-20°C, storage of the enzyme in the frozen state followed by subsequent thawing leads to a rapid decline in activity
-
-70°C, stored in buffer containing 200 mM KCl and 2 mg/ml bovine serum albumin, stable for more than 1 year
-
-80°C, 150 mM KCl, 20 mM Tris HCl, pH 7.4
-
-80°C, HEPES buffer, pH 7.5, 1 mM Lys, 10% (v/v) glycerol, several month, without any loss of activity
-80°C, HEPES buffer, pH 7.5, 1 mm Lys, 10% (v/v) glycerol, several months
-80°C, stored frozen without noticeable loss of enzyme activity
23°C, more than 90% stable for up to 1 week
-
25°C, aspartokinase I, stable at room temperature either in presence of 1.0 mM L-threonine or of 0.15 M KCl
-
4°C, all activity is lost in 1 day
-
4°C, dissolved in 0.05 M TES buffer containing 30% glycerol, pH 8.0, activity remains unchanged after 6 days
-
4°C, during 24 h of storage the loss of enzyme activity is substantial
-
4°C, enzyme activity can be maintained for several months
-
4°C, purified enzyme stable for at least 2 weeks in gel filtration buffer
-
4°C, refolded enzyme, no loss of activity for 24 h
-
4°C, stable for more than 1 week
-
use of bicine buffer and addition of sorbitol, bovine serum albumin or poly(ethyleneglycol) 6000 allows storage of the partially purified enzyme as a freeze-dried powder with relatively small loss of activity upon rehydration
-