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2.7.2.4: aspartate kinase

This is an abbreviated version!
For detailed information about aspartate kinase, go to the full flat file.

Word Map on EC 2.7.2.4

Reaction

ATP
+
L-aspartate
=
ADP
 +
4-phospho-L-aspartate

Synonyms

AK, AK II, AK III, AK-HSDH, AK-HSDH I, AK-HSDH1, AK-HSDH2, AK-HseDH, AK-R7, AK-ts31d, AK1, AK2, AK3, AKbeta, AKII, AKIII, AKsyn, Ask, ASK1, ASK2, Ask_Ect, Ask_LysC, Asp kinase-homoserine dehydrogenase, aspartate kinase, aspartate kinase (phosphorylating), aspartate kinase 1, aspartate kinase 2, aspartate kinase 3, aspartate kinase beta, aspartate kinase I, aspartate kinase II, aspartate kinase III, aspartic kinase, aspartokinase, aspartokinase 1-homoserine dehydrogenase 1, aspartokinase 2, aspartokinase 3, aspartokinase I, aspartokinase II, aspartokinase III, aspartokinase-homoserine dehydrogenase, beta-aspartokinase, bifunctional aspartate kinase-homoserine dehydrogenase, BT, BTT, CgAK, dap-aspartokinase, HOM3 product, HOM3-R7 product, HOM3-ts31d product, HseDH, LT-aspartokinase, lysC, lysine-sensitive aspartokinase 3, More, MtbAKbeta, nspJ, Thr-sensitive aspartate kinase, thrA, thrA1, thrA2, TM_0547, XbAK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.4 aspartate kinase

Cloned

Cloned on EC 2.7.2.4 - aspartate kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 subunits, alpha and beta, encoded by an inframe overlapping gene, askAB genes cloned and expressed in Escherichia coli GT3
-
ask gene conferred to Escherichia coli DH5alpha transformants
-
ask-asd operon cloned
aspartokinase encoded by lysC, expressed in Escherichia coli HB101
-
aspartokinase gene (ask) by cloning into an Escherichia coli/Corynebacterium glutamicum shuttle expression vector. Recombinant vector is transformed into Escherichia coli DH5alpha and then into Corynebacterium glutamicum. The induction of recombinant vector by IPTG has an inhibitory effect on cell growth due to over-expression of the cloned gene. A 2fold increase in lysine production is observed by cloning of the ASK gene in Corynebacterium glutamicum rather than in Escherichia coli, due to the presence of lysine exporter channel which facilitates lysine extraction
-
both catalytic domains of the enzyme, performing each one of the enzyme activities, are expressed separately with or without the interface region, resulting in increased activity of each domain compared to the wild-type bifunctional holoenzyme, the isolated catalytic domains are no longer allosterically regulated, expression of hybrid holoenzyme AKIII-HDHI+
-
by functional complementation of a Saccharomyces cerevisiae strain mutated in its homoserine dehydrogenase gene (hom6), expression in Escherichia coli, two of the three isolated clones are also able to complement a mutant yeast aspartate kinase gene (hom3), expression of the AK-HSDH gene in Arabidopsis thaliana (meristematic cells, leaves and stamens)
-
cDNA located on chromosome 4, cloned in Escherichia coli DH5alpha, overproduced in Escherichia coli BL21pLysS
DR1365 gene of Deinococcus radiodurans is isolated, after PCR amplification and several steps the amplified DNA fragment is introduced into Deinococcus radiodurans ATCC13939 to generate a DR1365 disruptant.
expressed as N-terminal 6× His-tagged fusion proteins under control of the T5 promoter in Escherichia coli M15(pREP4), homologous expression of wild-type and mutant M68V in Streptomyces albulus CR1
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Codon plus-RIPL cells
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL and BL21 (DE3) cells as well as in Streptomyces lividans strain TK23
-
expressing mutant gene lysC1 alone in Corynebacterium glutamicum wild-type strain ATCC13869 leads to exclusive L-lysine accumulation, co-expressing hom1 and thrB1 with lysC1 shifts partial carbon flux from L-lysine to L-threonine with minor L-isoleucine and no L-homoserine accumulation, further co-expressing ilvA1 completely deplets L-threonine and strongly shifts carbon flux from L-lysine to L-isoleucine
-
expression in Escherichia coli
expression in Escherichia coli and as fusion protein with GST (glutathione-S-transferase) in Saccharomyces cerevisiae
-
expression in Escherichia coli BL21-CodonPlus(DE3)-RIL cells with pET-26b(+) vector
functional recombinant expression of His-tagged chimeric mutant BT3 in Escherichia coli strain BL21(DE3)
gene lysC, recombinant coexpression of aspartate kinase and adenylylsulfate reductase genes substantially increases sulfur amino acid levels in transgenic alfalfa, Medicago sativa cv. Baoding by transformation via Agrobacterium tumefaciens strain EHA105, real-time quantitative PCR expression analysis, amino acid contents,overview
gene lysC, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21
gene thrA, recombinant overexpression of enzyme mutant G433R in Escherichia coli strain Bl21
gene TM_0547 , sequence comparisons, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3)
gene yclM introduced into aspartate kinase deficient Escherichia coli cells
-
into pET-41a expression vector and expression in Escherichia coli Rosetta (DE3) cells
into plasmid pET15b, N-terminal His-tagged AKIII transformed into the B834 (DE3) met- strain, untagged AKIII ligated into plasmid pET3d and transformed into Escherichia coli BL21(DE3) pLysS for protein expression
-
into the NdeI/HindIII site of pET26b(+) and introduced into Escherichia coli BL21-CodonPlus(DE3)-RIL cells
into vector pCR2.1-TOPO, subcloned to pUC18 for heterologous expression of thrA1 or thrA2 in Escherichia coli Gif 102
-
into vector pET-26b(+), introduced into Escherichia coli BL21-CodonPlus(DE3)-RIL cells
into vector pET26b(+) and introduced into Escherichia coli BL21-CodonPlus(DE3)-RIL cells
ligated into pET 23d(+) vector and Escherichia coli strain DH10B for cloning, expression in Escherichia coli BL21 (DE3) pLysS codon+
ligated into pET 23d(+) vector, expressed in Escherichia coli BL21 codon (+)
lysC geneencoding for AKIII cloned into vector pTZ19U and expressed in the protease-deficient Escherichia coli BL21DE3
-
mutant enzymes are expressed in Escherichia coli BL21 cells
operon encoding aspartokinase cloned and sequenced, introduced into auxotrophic Escherichia coli CGSC5074 and Escherichia coli X6118 and functionally expressed
-
pET-41a vector and Rosetta (DE3) Escherichia coli cells for protein expression
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli TOP10 cells
recombinantly expressed in Escherichia coli
-
sequence comparisons, recombinant expression of wild-type and mutant enzymes
single aspartate kinase encoded by the HOM3 gene, Escherichia coli DH5alpha as host
-
The BamHI-PstI 6.5-kb fragment is cloned and sequenced. The ApaI 1.9-kb fragment carrying the Ask genes (wild type and M68V mutant) is cloned into the pLAE003 plasmid. The resulting plasmids are used for homologous expression in Streptomyces albulus CR1. The PCR amplified fragments carrying the Ask genes (wild type and M68V mutant) with their own ribosome binding site (RBS) are cloned into the pLAE006 plasmid. The resulting plasmids are used for the homologous expression in Streptomyces albulus CR1. The pQE30 plasmid and Escherichia coli M15 (pREP4) are used to overexpress the recombinant protein.
the gene is cloned into the NheI and BamHI restriction enzyme sites of the expression vector pET28 to create pET28+AKSc, expression in Escherichia coli
-
the monofunctional AK, Ask1 and Ask2 coding sequences are cloned using RNA from the B73 inbred, the two maize genes share a high degree of identity with each other and similar genes of other species, Ask2-Oh545o2 and Ask2-Oh51Ao2 sequences are expressed in Saccharomyces cerevisiae hom3/ura3 mutant sigma a3hu, there is one amino acid difference between the Ask2 alleles, Ask2-Oh545o2 and Ask2-Oh51Ao2 have similar lysine-sensitivity properties but differ in basal activity, the Ask2 gene is tightly linked to the QTL on chromosome 2 that is associated with a high endosperm lysine content. There is one amino acid difference in the Ask2 enzymes of Oh545o2 and Oh51Ao2.
yeast hom3/ura3 mutant sigmaa3hu was transformed with plasmids expressing the maize Ask2-Oh545o2 and Ask2-Oh51Ao2 sequences