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2.7.2.4: aspartate kinase

This is an abbreviated version!
For detailed information about aspartate kinase, go to the full flat file.

Word Map on EC 2.7.2.4

Reaction

ATP
+
L-aspartate
=
ADP
 +
4-phospho-L-aspartate

Synonyms

AK, AK II, AK III, AK-HSDH, AK-HSDH I, AK-HSDH1, AK-HSDH2, AK-HseDH, AK-R7, AK-ts31d, AK1, AK2, AK3, AKbeta, AKII, AKIII, AKsyn, Ask, ASK1, ASK2, Ask_Ect, Ask_LysC, Asp kinase-homoserine dehydrogenase, aspartate kinase, aspartate kinase (phosphorylating), aspartate kinase 1, aspartate kinase 2, aspartate kinase 3, aspartate kinase beta, aspartate kinase I, aspartate kinase II, aspartate kinase III, aspartic kinase, aspartokinase, aspartokinase 1-homoserine dehydrogenase 1, aspartokinase 2, aspartokinase 3, aspartokinase I, aspartokinase II, aspartokinase III, aspartokinase-homoserine dehydrogenase, beta-aspartokinase, bifunctional aspartate kinase-homoserine dehydrogenase, BT, BTT, CgAK, dap-aspartokinase, HOM3 product, HOM3-R7 product, HOM3-ts31d product, HseDH, LT-aspartokinase, lysC, lysine-sensitive aspartokinase 3, More, MtbAKbeta, nspJ, Thr-sensitive aspartate kinase, thrA, thrA1, thrA2, TM_0547, XbAK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.4 aspartate kinase

Purification

Purification on EC 2.7.2.4 - aspartate kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoenzymes partially purified
-
aspartokinase devoid of homoserine dehydrogenase activity extracted from mutant Gif 108
-
aspartokinase III
-
aspartokinase-homoserine dehydrogenase complex
-
by anion-exchange chromatography and gel filtration, more than 99% pure
by Ni2+-affinity chromatography and gel filtration
by nickel-affinity chromatography and gel filtration, to more than 95% homogeneity
extraction from cotyledons
-
His-tagged AKIII by nickel affinity chromatography followed by gel filtration, more than 99%, untagged AKIII by gel filtration, more than 98%
-
native and recombinant enzyme
Ni Sepharose column chromatography
-
Ni-NTA column chromatography, and Superdex 200 gel filtration
-
Ni-NTA resin column chromatography and Superdex 200 gel filtration
Ni-Sepharose column chromatography, and Superdex 200 gel filtration
-
Ni2+-affinity with Ni2+-NTA resin and gel-filtration chromatogrphy
nickel ion affinity column chromatography
-
nickel-affinity chromatography, gel-filtration FPLC column, more than 95% homogeneity of the purified subunit is verified by SDS-PAGE
nickel-nitriloacetic acid (Ni-NTA) affinity chromatography
on anion exchange column and by gel filtration
partial
partially
partially purified, 29.9fold, by gel filtration
-
partially, 2 isoenzymes
-
recombinant C-terminally His6-tagged enzyme from Escherichia coli TOP10 cells by nickel affinity chromatography and gel filtration
recombinant enzyme
-
recombinant enzyme from E. coli
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment, hydrophobic interaction chromatography, gel filtration, hydroxyapatite chromatography, and ultrafiltration
recombinant enzyme, expressed in E. coli
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant protein, expressed in Escherichia coli Gif106M1
-
threonine-sensitive aspartokinase, copurified with homoserine dehydrogenase, 3 isoenzymes, threonine, lysine and lysine plus S-adenosylmethionine-sensitive, purified
-
to near homogeneity
Toyopearl ether-650M column chromatography, Superdex 200 gel filtration and Cellulofine HAP hydroxyl apatite column chromatography
two successive chromatography steps: first a high resolution anionic exchange resin, followed by gel filtration column, the resulting protein is shown by SDS-PAGE to be more than 99% pure, concentration to 30 mg/ml by using a 10000 molecular weight cut-off Amicon concentrator
using Ni-NTA chromatography
VB217, aspartokinase II
-
weak anion exchange chromatography, affinity chromatogrphy for AK1
weak anion exchange chromatography, gel filtration