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2.4.2.1: purine-nucleoside phosphorylase

This is an abbreviated version!
For detailed information about purine-nucleoside phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.1

Reaction

purine 2'-deoxyribonucleoside
+
phosphate
=
purine
+
2-deoxy-alpha-D-ribose 1-phosphate

Synonyms

5'-deoxy-5'-methylthioadenosine phosphorylase II, 5'-methythioadenosine phosphorylase, adenine nucleoside phosphorylase, adenosine phosphorylase, AgPNP, AmPNP, ANP, ApMTAP, apPNP, bovPNP, bPNO, CKP1, cytokinin riboside phosphorylase, DeoD, deoD_1, DgPNP, EC592_06680, EcPNP, flB, GsPNPI, GsPNPII, GtPNP, hPNP, HpPNP, HsPNO, HsPNP, Ino-Guo phosphorylase, inosine phosphorylase, inosine-guanosine phosphorylase, KlacPNP, KLLA0_C16621g, MTAPII, MtbPNP, nucleotide phosphatase (2.4.2.1), PfPNP, phosphorylase, purine nucleoside, PiPNP, PNP, PNP 1, PNP 2, PNP I, PNP II, PNP-II, PNP-Zg, PNPase, pNPI, PNPII, Pu-NPase, punA, PUNP, PUNPI, PUNPII, purine deoxynucleoside phosphorylase, purine deoxyribonucleoside phosphorylase, purine nucleoside orthophosphate ribosyl transferase, purine nucleoside phosphorylase, purine nucleoside phosphorylase 1, purine nucleoside phosphorylase 2, purine nucleoside phosphorylase DeoD, purine nucleoside phosphorylase DeoD-type, purine nucleoside phosphorylase II, purine ribonucleoside phosphorylase, purine-nucleoside phosphorylases II, SalT, SSO2343, TgPNP, TTC0194, TTC1070, TtPNPI, TtPNPII, TvPNP, xanthosine phosphorylase, XAP, xapA, ylmD

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.1 purine-nucleoside phosphorylase

Molecular Weight

Molecular Weight on EC 2.4.2.1 - purine-nucleoside phosphorylase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
gel filtration
102000 - 108000
-
-
105000
recombinant His-tagged enzyme, gel filtration
112000
-
gel filtration
113000
-
gel filtration
114000
-
gel filtration
120000
-
non-denaturing PAGE
121800
-
non-denaturing PAGE
128800
-
gel filtration
138000
142000
recombinant enzyme, gel filtration
147000
-
sucrose density gradient centrifugation
150000
-
sedimentation equilibrium analysis
153000
-
adenosine-specific phosphorylase, gel filtration
154716
sequence comparison
156000
164200
gel filtration
168000
-
gel filtration
170000
-
gel filtration
180000
23700
23900
-
5 * 23900, SDS-PAGE
24000
25000
x * 25000, calculated and SDS-PAGE
25018
x * 25018, calculated from sequence
25415
6 * 25415, calculated
25500
-
6 * 25500, adenosine-specific phosphorylase, SDS-PAGE
25817
-
6 * 25817, electrospray ionizing mass spectrometry
27000
28000
28977
6 * 28977, calculated from sequence
29000
29035
-
3 * 29035, mass spectrometry
29208
6 * 29208, calculated from sequence
29700
-
3 * 29700, SDS-PAGE
30000
30500
-
2 * 30500, SDS-PAGE
31000
-
1 * 31000, SDS-PAGE in absence and presence of 2-mercaptoethanol
31600
-
3 * 31600, SDS-PAGE
31654
3 * 31654, calculated from amino acid sequence
32000
33000
-
x * 33000, recombinant protein including His-tag
34000
-
2 * 34000, SDS-PAGE
345000
-
2 * 345000, SDS-PAGE
38000
-
2 * 38000, SDS-PAGE
39000
-
x * 39000, SDS-PAGE
44000
-
2 * 44000, SDS-PAGE
45000
-
gel filtration
56000 - 58000
-
-
58000
-
non-denaturing PAGE
61000
-
gel filtration
67000
-
equilibrium sedimentation, gel filtration, amino acid analysis
68000
70000
-
gel filtration
75000 - 83000
-
sucrose density gradient centrifugation
78000 - 80000
-
gel filtration
80000 - 92000
-
gel filtration, equilibrium sedimentation
81000
-
gel filtration
84000
-
gel filtration
87000
89000
90000
91000
-
equilibrium sedimentation
93800
-
disc gel electrophoresis
94000
95000
-
inosine-guanosine phosphoylase, gel filtration