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Literature summary for 2.4.2.1 extracted from
- Rabbit brain purine nucleoside phosphorylase. Physical and chemical properties. Inhibition studies with aminopterin, folic acid and structurally related compounds (1978), Arch. Biochem. Biophys., 190, 662-670.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-aza-2,6-diaminopurine | noncompetitive | Oryctolagus cuniculus |  |
| aminopterin | noncompetitive | Oryctolagus cuniculus | |
| D,L-6-methyl 5,6,7,8-tetrahydropterin | competitive | Oryctolagus cuniculus | |
| erythro-9-(2-hydroxy-3-nonyl)adenine | competitive | Oryctolagus cuniculus | |
| folate | competitive | Oryctolagus cuniculus | |
| PCMB | 0.1 mM, 85% loss of activity within 15 min | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
guanosine | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Oryctolagus cuniculus |
| 345000 | - |
2 * 345000, SDS-PAGE | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| high sensitivity to photooxidation in presence of methylene blue, almost 95% loss of activity after 40 min irradiation | Oryctolagus cuniculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanosine + phosphate | - |
Oryctolagus cuniculus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 345000, SDS-PAGE | Oryctolagus cuniculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0337 | - |
folic acid | - |
Oryctolagus cuniculus | |
| 0.038 | - |
2-amino-6-methyl-5,6,7,8-tetrahydropteridin-4(1H)-one | - |
Oryctolagus cuniculus | |
| 0.15 | - |
aminopterin | - |
Oryctolagus cuniculus | |
| 0.19 | - |
erythro-9-(2-hydroxy-3-nonyl)adenine | - |
Oryctolagus cuniculus | |
| 0.195 | - |
8-aza-2,6-diaminopurine | - |
Oryctolagus cuniculus | |
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