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2.4.2.1: purine-nucleoside phosphorylase

This is an abbreviated version!
For detailed information about purine-nucleoside phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.1

Reaction

purine 2'-deoxyribonucleoside
+
phosphate
=
purine
 +
2-deoxy-alpha-D-ribose 1-phosphate

Synonyms

5'-deoxy-5'-methylthioadenosine phosphorylase II, 5'-methythioadenosine phosphorylase, adenine nucleoside phosphorylase, adenosine phosphorylase, AgPNP, AmPNP, ANP, ApMTAP, apPNP, bovPNP, bPNO, CKP1, cytokinin riboside phosphorylase, DeoD, deoD_1, DgPNP, EC592_06680, EcPNP, flB, GsPNPI, GsPNPII, GtPNP, hPNP, HpPNP, HsPNO, HsPNP, Ino-Guo phosphorylase, inosine phosphorylase, inosine-guanosine phosphorylase, KlacPNP, KLLA0_C16621g, MTAPII, MtbPNP, nucleotide phosphatase (2.4.2.1), PfPNP, phosphorylase, purine nucleoside, PiPNP, PNP, PNP 1, PNP 2, PNP I, PNP II, PNP-II, PNP-Zg, PNPase, pNPI, PNPII, Pu-NPase, punA, PUNP, PUNPI, PUNPII, purine deoxynucleoside phosphorylase, purine deoxyribonucleoside phosphorylase, purine nucleoside orthophosphate ribosyl transferase, purine nucleoside phosphorylase, purine nucleoside phosphorylase 1, purine nucleoside phosphorylase 2, purine nucleoside phosphorylase DeoD, purine nucleoside phosphorylase DeoD-type, purine nucleoside phosphorylase II, purine ribonucleoside phosphorylase, purine-nucleoside phosphorylases II, SalT, SSO2343, TgPNP, TTC0194, TTC1070, TtPNPI, TtPNPII, TvPNP, xanthosine phosphorylase, XAP, xapA, ylmD

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.1 purine-nucleoside phosphorylase

Specific Activity

Specific Activity on EC 2.4.2.1 - purine-nucleoside phosphorylase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00075
-
substrate adenosine, pH 7.4
0.00082
-
unpurified enzyme, with 5'-deoxy-5'-methylthioadenosine as substrate, at pH 7.0 and 37°C
0.015
-
wild-type enzyme, substrate 9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine
0.016
substrate 5'-methylthioinosine, pH 7.4
0.0167
-
mutant M64V, substrate 9-[beta-D-arabinofuranosyl]-2-fluoro-adenine
0.0179
0.02
-
wild-type enzyme, substrate 9-[beta-D-arabinofuranosyl]-2-fluoro-adenine
0.023
substrate inosine, pH 7.4
0.0307
inosine as a variable substrate
0.034
-
purified enzyme, with 5'-deoxy-5'-methylthioadenosine as substrate, at pH 7.0 and 37°C
0.041
substrate inosine, mutant R217A, pH 7.0, 25°C
0.05
substrate adenosine, mutant D204A, pH 7.0, 25°C
0.06
substrate guanosine, mutant D204A, pH 7.0, 25°C
0.14
substrate inosine, mutant D204A/R217A, pH 7.0, 25°C
0.18
substrate inosine, mutant R24A, pH 7.0, 25°C
0.567
-
wild-type enzyme, substrate 9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine
0.95
-
substrate 2'-deoxyinosine, pH 7.4
1.17
-
substrate 2'-deoxyguanosine, pH 7.4
1.4
substrate inosine, mutant D204N, pH 7.0, 25°C
1.43
-
mutant M64V, substrate 9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine
10
-
substrate adenosine, crude cell extract, pH 7.5, temperature not specified in the publication
100
-
inosine-guanosine phosphorylase
103.2
substrate inosine, wild-type, pH 7.0, 25°C
12
-
presence of 100 mM phosphate, pH 7.0
13.8
purified enzyme, pH 7.0, 25°C, substrates guanosine and phosphate
139
-
presence of 50 mM phosphate, pH 7.0
2.1
substrate guanosine, mutant D204N, pH 7.0, 25°C
21.3
substrate 7-methylguanosine, mutant R217A, pH 7.0, 25°C
21.4
substrate 7-methylguanosine, wild-type, pH 7.0, 25°C
23.3
-
mutant M64V, substrate 9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine
26.67
-
mutant M64V, substrate 2-fluoro-2'-deoxyadenosine
2630
-
substrate adenosine, crude cell extract, pH 7.5, temperature not specified in the publication
2890
-
substrate inosine, crude cell extract, pH 7.5, temperature not specified in the publication
3.47
-
substrate inosine, pH 7.4
30.23
Halalkalibacterium halodurans
pH 7.5, substrate inosine
370
-
substrate guanosine, crude cell extract, pH 7.5, temperature not specified in the publication
3740
-
substrate inosine, crude cell extract, pH 7.5, temperature not specified in the publication
4
-
mutant M64V, substrate 9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine
4.55
-
-
41
-
presence of 50 mM phosphate, pH 7.0
46
-
presence of 100 mM phosphate, pH 7.0
46.9
substrate 7-methylguanosine, mutant D204A/R217A, pH 7.0, 25°C
47
-
presence of 100 mM phosphate, pH 7.0
49.4
purified enzyme, pH 7.0, 25°C, substrates inosine and phosphate
53.3
-
-
5420
-
substrate guanosine, crude cell extract, pH 7.5, temperature not specified in the publication
55
-
reaction with guanosine
58.5
substrate adenosine, wild-type, pH 7.0, 25°C
6
purified enzyme, pH 7.0, 25°C, substrates adenosine and phosphate
6.3
substrate adenosine, mutant R217A, pH 7.0, 25°C
6.4
substrate 7-methylguanosine, mutant R24A, pH 7.0, 25°C
6.9
substrate 7-methylguanosine, mutant D204N, pH 7.0, 25°C
60.4
substrate guanosine, wild-type, pH 7.0, 25°C
7.3
-
wild-type enzyme, substrate 2-fluoro-2'-deoxyadenosine
7.5
purified recombinant enzyme, pH 7.0, 25°C, substrate 7-methylguanosine
73
-
adenosine-specific phosphorylase
77.5
-
-
78.3
-
-
8.83
-
wild-type enzyme, substrate 9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine
80.4
purified recombinant enzyme, pH 7.0, 25°C, substrate inosine
9.8
purified enzyme, pH 7.0, 25°C, substrates 7-methylguanosine and phosphate
9.83
-
mutant M64V, substrate 9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine
additional information