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Literature summary for 2.4.2.1 extracted from
- Chinese hamster purine-nucleoside phosphorylase: purification, structural, and catalytic properties (1976), Biochemistry, 15, 4967-4973.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
hypoxanthine | - |
Cricetulus griseus |  |
| 0.035 | - |
guanine | - |
Cricetulus griseus | |
| 0.05 | - |
Inosine | - |
Cricetulus griseus | |
| 0.2 | - |
phosphate | - |
Cricetulus griseus | |
| 0.2 | - |
alpha-D-ribose 1-phosphate | - |
Cricetulus griseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, enzyme behaves as a mixture of dimers and trimers, SDS-PAGE | Cricetulus griseus |
| 30000 | - |
3 * 30000, enzyme behaves as a mixture of dimers and trimers, SDS-PAGE | Cricetulus griseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cricetulus griseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Cricetulus griseus | - |
| liver | - |
Cricetulus griseus | - |
| V-79 cell | - |
Cricetulus griseus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Cricetulus griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanosine + phosphate | - |
Cricetulus griseus | guanine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Cricetulus griseus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30000, enzyme behaves as a mixture of dimers and trimers, SDS-PAGE | Cricetulus griseus |
| More | enzyme behaves as a mixture of dimers of 68000 Da and trimers of 89000 Da | Cricetulus griseus |
| trimer | 3 * 30000, enzyme behaves as a mixture of dimers and trimers, SDS-PAGE | Cricetulus griseus |
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