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2.4.2.1: purine-nucleoside phosphorylase

This is an abbreviated version!
For detailed information about purine-nucleoside phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.1

Reaction

purine 2'-deoxyribonucleoside
+
phosphate
=
purine
 +
2-deoxy-alpha-D-ribose 1-phosphate

Synonyms

5'-deoxy-5'-methylthioadenosine phosphorylase II, 5'-methythioadenosine phosphorylase, adenine nucleoside phosphorylase, adenosine phosphorylase, AgPNP, AmPNP, ANP, ApMTAP, apPNP, bovPNP, bPNO, CKP1, cytokinin riboside phosphorylase, DeoD, deoD_1, DgPNP, EC592_06680, EcPNP, flB, GsPNPI, GsPNPII, GtPNP, hPNP, HpPNP, HsPNO, HsPNP, Ino-Guo phosphorylase, inosine phosphorylase, inosine-guanosine phosphorylase, KlacPNP, KLLA0_C16621g, MTAPII, MtbPNP, nucleotide phosphatase (2.4.2.1), PfPNP, phosphorylase, purine nucleoside, PiPNP, PNP, PNP 1, PNP 2, PNP I, PNP II, PNP-II, PNP-Zg, PNPase, pNPI, PNPII, Pu-NPase, punA, PUNP, PUNPI, PUNPII, purine deoxynucleoside phosphorylase, purine deoxyribonucleoside phosphorylase, purine nucleoside orthophosphate ribosyl transferase, purine nucleoside phosphorylase, purine nucleoside phosphorylase 1, purine nucleoside phosphorylase 2, purine nucleoside phosphorylase DeoD, purine nucleoside phosphorylase DeoD-type, purine nucleoside phosphorylase II, purine ribonucleoside phosphorylase, purine-nucleoside phosphorylases II, SalT, SSO2343, TgPNP, TTC0194, TTC1070, TtPNPI, TtPNPII, TvPNP, xanthosine phosphorylase, XAP, xapA, ylmD

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.1 purine-nucleoside phosphorylase

Cloned

Cloned on EC 2.4.2.1 - purine-nucleoside phosphorylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(lambdaDE3) cells
expressed in Escherichia coli BL21-Codon plus cells
expressed in Escherichia coli Rosetta(DE3) cells
-
expression in Escherichia coli
expression in Escherichia coli BL21
-
expression in Escherichia coli BL21 (DE3). The enzyme shows high activity and behavior similar to the protein isolated from mammalian tissue. Thus it can fully replace the non-recombinant PNP
-
expression of site-directed mutants in Escherichia coli
-
expression of the Escherichia coli purine nucleoside phosphorylase/9-(2-deoxy-beta-dribofuranosyl)-6-methylpurine suicide gene system in human hepatocarcinoma cells
-
gene deoD, DNA and amino acid sequence determination and analysis of clinical isolate PNP-Zg encoded by a deoD gene, sequence comparisons, recombinant expression of PNP-Zg in Escherichia coli strain BL21-CodonPlus(DE3)-RIL
gene deoD, recombinant expression of the enzyme in Escherichia coli cell strain BL21-CodonPlus(DE3)-RIL
gene pnp, sequence comparisons of Escherichia coli enzyme with human and Bacillus subtilis enzymes, recombinant expression in Escherichia coli strain BL21(DE3)/pERPUPHOI
overexpression in Escherichia coli
-
sequence comparisons, recombinant expression of N- and C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli
trans-activator of transcription-mediated long-term intracellular delivery of purine nucleoside phosphorylase corrects its deficiency in mice