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0.01 - 10.5
2',3'-dideoxyinosine
5.1 - 830
2'-deoxyguanosine
0.9 - 627
2'-deoxyinosine
17.3
2-amino-6-mercapto-7-methylpurine ribonucleoside
-
-
0.155 - 2.77
2-fluoro-2'-deoxyadenosine
2.05
2-fluoroadenosine
pH 7.4, 25°C
0.027 - 2.6
5'-methylthioadenosine
0.01 - 1.2
5'-methylthioinosine
6.23 - 73.1
7-methylguanosine
2.57 - 27.5
7-Methylinosine
9.7
9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine
pH 7.4, 25°C
1.1
9-beta-D-ribofuranosyl-6-methylthiopurine
pH 7.4, 25°C
115 - 145
alpha-D-deoxyribose 1-phosphate
100 - 120
alpha-D-ribose 1-phosphate
0.2 - 3.4
Methylthioinosine
0.001 - 1
p-nitrophenyl beta-D-ribofuranoside
additional information
additional information
-
0.01
2',3'-dideoxyinosine
mutant Y88E, pH 7.0, 25°C
0.02
2',3'-dideoxyinosine
mutant Y88D, pH 7.0, 25°C
0.09
2',3'-dideoxyinosine
mutant Y88W, pH 7.0, 25°C
0.15
2',3'-dideoxyinosine
mutant Y88Q, pH 7.0, 25°C
0.4
2',3'-dideoxyinosine
mutant Y88T, pH 7.0, 25°C
0.5
2',3'-dideoxyinosine
mutant Y88I, pH 7.0, 25°C
0.6
2',3'-dideoxyinosine
mutant Y88V, pH 7.0, 25°C
0.9
2',3'-dideoxyinosine
wild-type, pH 7.0, 25°C
1.5
2',3'-dideoxyinosine
mutant Y88M, pH 7.0, 25°C
2.5
2',3'-dideoxyinosine
mutant Y88C, pH 7.0, 25°C
2.5
2',3'-dideoxyinosine
mutant Y88H, pH 7.0, 25°C
2.7
2',3'-dideoxyinosine
mutant Y88S, pH 7.0, 25°C
2.8
2',3'-dideoxyinosine
mutant Y88A, pH 7.0, 25°C
5.2
2',3'-dideoxyinosine
mutant Y88M, pH 7.0, 25°C
6.1
2',3'-dideoxyinosine
mutant Y88L, pH 7.0, 25°C
10.5
2',3'-dideoxyinosine
mutant Y88F, pH 7.0, 25°C
5.1
2'-deoxyguanosine
-
pH 7.0, 25°C
830
2'-deoxyguanosine
pH 7.0, 80°C
0.9
2'-deoxyinosine
-
pH 7.4
1.2
2'-deoxyinosine
-
pH 7.4
7.5
2'-deoxyinosine
-
pH 7.0, 25°C
627
2'-deoxyinosine
pH 7.0, 80°C
0.155
2-fluoro-2'-deoxyadenosine
-
mutant E201Q/N243D
2.77
2-fluoro-2'-deoxyadenosine
pH 7.4, 25°C
0.027
5'-methylthioadenosine
pH 7.4
2.6
5'-methylthioadenosine
pH 7.4
0.01
5'-methylthioinosine
mutant enzyme V66I/Y73I/Y160F, at pH 7.4, temperature not specified in the publication
0.05
5'-methylthioinosine
mutant enzyme V66I/Y160F, at pH 7.4, temperature not specified in the publication
0.28
5'-methylthioinosine
mutant enzyme V73I/Y160F, at pH 7.4, temperature not specified in the publication
0.3
5'-methylthioinosine
mutant enzyme Y160F, at pH 7.4, temperature not specified in the publication
0.81
5'-methylthioinosine
mutant enzyme V73I, at pH 7.4, temperature not specified in the publication
0.83
5'-methylthioinosine
wild type enzyme, at pH 7.4, temperature not specified in the publication
0.91
5'-methylthioinosine
mutant enzyme V66I/V73I, at pH 7.4, temperature not specified in the publication
1.2
5'-methylthioinosine
mutant enzyme V66I, at pH 7.4, temperature not specified in the publication
6.23
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
9.2
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
20.4
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
23
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
24.7
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
73.1
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
2.57
7-Methylinosine
-
-
18
7-Methylinosine
-
pH 7.0, 25°C
0.22
adenosine
-
mutant hexameric enzyme N204D, at pH 8.0 and 70°C
0.282
adenosine
-
mutant enzyme N243D, pH 7.0
0.55
adenosine
-
wild type trimeric enzyme, at pH 8.0 and 70°C
0.579
adenosine
-
mutant enzyme N243D/K244Q, pH 7.0
0.71
adenosine
-
mutant enzyme N243D, pH 6.0
1.14
adenosine
-
mutant enzyme N243D/K244Q, pH 6.0
1.41
adenosine
-
mutant enzyme N243D/K244Q, pH 6.0
1.7
adenosine
-
25°C, pH 7.4
3.3
adenosine
pH 7.4, 25°C
14.3
adenosine
pH 8.0, 50°C, recombinant enzyme
20
adenosine
80°C, pH 7.0
22.8
adenosine
recombinant mutant N256D, pH 7.0, 25°C
3450
adenosine
pH 7.0, 90°C
115
alpha-D-deoxyribose 1-phosphate
-
reaction with hypoxanthine
145
alpha-D-deoxyribose 1-phosphate
-
reaction with guanine
100
alpha-D-ribose 1-phosphate
-
reaction with hypoxanthine
120
alpha-D-ribose 1-phosphate
-
reaction with guanine
0.48
deoxyinosine
pH 7.4
48
guanine
recombinant erythrocyte enzyme
127
guanine
-
reaction with ribose 1-phosphate
158
guanine
-
reaction with deoxyribose 1-phosphate
0.25
guanosine
-
mutant trimeric enzyme A196E/D238N, at pH 8.0 and 70°C
0.26
guanosine
-
wild type hexameric enzyme, at pH 8.0 and 70°C
1.1
guanosine
-
25°C, pH 7.4
2 - 8
guanosine
recombinant erythrocyte enzyme
4
guanosine
-
pH 7.0, 25°C
7.8
guanosine
pH 7.4, 25°C, wild-type heavy PNP
8.3
guanosine
pH 7.4, 25°C, mutant F159Y heavy PNP
8.5
guanosine
pH 7.4, 25°C, mutant F159Y light PNP
8.7
guanosine
pH 7.4, 25°C, wild-type light PNP
10.4
guanosine
recombinant mutant N256D, pH 7.0, 25°C
12
guanosine
Halalkalibacterium halodurans
pH 7.5
13.4
guanosine
-
native enzyme, at pH 7.4 and 25°C
27
guanosine
-
pH 7.4, 25°C
29.7
guanosine
recombinant wild-type enzyme, pH 7.0, 25°C
30
guanosine
-
mutant L22E/H104R, pH 7.4, 25°C
31
guanosine
-
wild-tpe, pH 7.4, 25°C
60
guanosine
-
mutant E201Q/N243D
296
guanosine
pH 7.0, 80°C
876
guanosine
pH 7.0, 90°C
18400
guanosine
pH 8.0, 50°C, recombinant enzyme
0.5
hypoxanthine
-
mutant enzyme R84A
0.97
hypoxanthine
-
mutant enzyme H86A
1.4
hypoxanthine
-
mutant enzyme E201A
2.1
hypoxanthine
-
mutant enzyme S33A
9
hypoxanthine
-
mutant enzyme Y88F
10
hypoxanthine
-
mutant enzyme H257A
13
hypoxanthine
-
mutant enzyme N243A
14
hypoxanthine
-
mutant enzyme F200A
24
hypoxanthine
-
mutant enzyme F159A
58
hypoxanthine
-
mutant enzyme T242A
98
hypoxanthine
-
mutant enzyme K244A
103
hypoxanthine
-
reaction with ribose 1-phosphate
110
hypoxanthine
-
wild-type enzyme
110
hypoxanthine
recombinant erythrocyte enzyme
127
hypoxanthine
-
reaction with deoxyribose 1-phosphate
130
hypoxanthine
-
mutant enzyme M219A
0.01
Inosine
mutant Y88R, pH 7.0, 25°C
0.06
Inosine
-
mutant enzyme N243A
0.21
Inosine
mutant enzyme Y160F, at pH 7.4, temperature not specified in the publication
0.23
Inosine
pH 7.4, temperature not specified in the publication
0.36
Inosine
mutant enzyme V66I/Y73I/Y160F, at pH 7.4, temperature not specified in the publication
0.386
Inosine
-
mutant enzyme N243D, pH 7.0
0.59
Inosine
mutant enzyme V73I/Y160F, at pH 7.4, temperature not specified in the publication
0.7
Inosine
mutant Y88E, pH 7.0, 25°C
0.8
Inosine
mutant Y88D, pH 7.0, 25°C
0.9
Inosine
mutant T90A/T156A, pH 7.6, 37°C
1.1
Inosine
-
mutant enzyme S33A
1.2
Inosine
mutant T90A, pH 7.6, 37°C
1.2
Inosine
mutant enzyme V66I/Y160F, at pH 7.4, temperature not specified in the publication
1.3
Inosine
mutant Y88I, pH 7.0, 25°C
1.4
Inosine
mutant Y88Q, pH 7.0, 25°C
1.7
Inosine
-
pH 7.5, 25°C
1.7
Inosine
-
pH 7.4, temperature not specified in the publication
1.7
Inosine
wild type enzyme, at pH 7.4, temperature not specified in the publication
1.75
Inosine
25°C, pH 7.4
1.76
Inosine
-
mutant enzyme N243D, pH 6.0
1.8
Inosine
-
25°C, pH 7.4
1.9
Inosine
mutant enzyme V73I, at pH 7.4, temperature not specified in the publication
2
Inosine
mutant enzyme V66I, at pH 7.4, temperature not specified in the publication
2.1
Inosine
-
mutant enzyme R84A
2.3
Inosine
mutant Y88T, pH 7.0, 25°C
2.4
Inosine
mutant Y88V, pH 7.0, 25°C
4.2
Inosine
-
mutant enzyme E201A
4.6
Inosine
mutant enzyme V66I/V73I, at pH 7.4, temperature not specified in the publication
5.4
Inosine
-
pH 7.0, 25°C
5.5
Inosine
mutant Y88C, pH 7.0, 25°C
5.6
Inosine
mutant Y88W, pH 7.0, 25°C
6.55
Inosine
25°C, pH 7.4
8.6
Inosine
mutant Y88H, pH 7.0, 25°C
8.8
Inosine
mutant Y88S, pH 7.0, 25°C
9.1
Inosine
-
mutant enzyme N243D/K244Q, pH 7.0
9.4
Inosine
-
mutant enzyme F200A
9.7
Inosine
mutant Y88M, pH 7.0, 25°C
12.8
Inosine
mutant Y88M, pH 7.0, 25°C
14
Inosine
-
mutant enzymes T242A and H86A
15.7
Inosine
-
pH 7.5, 37°C
15.9
Inosine
mutant Y88L, pH 7.0, 25°C
16
Inosine
-
mutant enzyme H257A
17.6
Inosine
mutant Y88A, pH 7.0, 25°C
20
Inosine
-
pH 7.4, 25°C
25
Inosine
mutant N123L/R210Q, pH 7.5, 25°C
25.1
Inosine
recombinant mutant N256E, pH 7.0, 25°C
28.3
Inosine
mutant Y88F, pH 7.0, 25°C
31.5
Inosine
-
mutant L22E/H104R, pH 7.4, 25°C
32
Inosine
-
mutant enzyme F159A
34.9
Inosine
recombinant mutant N256D, pH 7.0, 25°C
36.4
Inosine
-
mutant enzyme N243D/K244Q, pH 6.0
38
Inosine
mutant V39T/N123L/R210Q, pH 7.5, 25°C
38.9
Inosine
-
wild-type enzyme, pH 6.0
40
Inosine
-
wild-tpe, pH 7.4, 25°C
43.9
Inosine
wild-type, pH 7.0, 25°C
43.9
Inosine
recombinant wild-type enzyme, pH 7.0, 25°C
44
Inosine
wild-type, pH 7.5, 25°C
46
Inosine
-
mutant enzyme E89A
47.3
Inosine
-
wild-type enzyme, pH 7.0
49
Inosine
-
mutant enzyme K244A
52
Inosine
-
mutant enzyme M219A
54
Inosine
-
mutant enzyme Y88F
56
Inosine
-
wild-type enzyme
56
Inosine
recombinant erythrocyte enzyme
56.4
Inosine
mutant T156A, pH 7.6, 37°C
62.3
Inosine
mutant T90S, pH 7.6, 37°C
82.4
Inosine
mutant V206I, pH 7.6, 37°C
84.1
Inosine
pH 7.6, 37°C
84.1
Inosine
wild-type, pH 7.6, 37°C
98.4
Inosine
mutant T156S, pH 7.6, 37°C
164.4
Inosine
mutant T90R, pH 7.6, 37°C
324.3
Inosine
mutant T90R/T156A, pH 7.6, 37°C
3900
Inosine
-
isoform of 702 amino acids, pH 7.0, 37°C
6200
Inosine
-
pH 7.0, 37°C
7000
Inosine
-
pH 7.0, 37°C
12000
Inosine
-
isoform of 816 amino acids, pH 7.0, 37°C
28000
Inosine
Halalkalibacterium halodurans
pH 7.5
63800
Inosine
pH 8.0, 50°C, recombinant enzyme
0.2
Methylthioinosine
pH 7.5, 25°C
1.5
Methylthioinosine
-
pH 7.5, 25°C
2.6
Methylthioinosine
-
pH 7.4
3.4
Methylthioinosine
-
pH 7.4
0.001
p-nitrophenyl beta-D-ribofuranoside
-
25°C, pH 7.4
1
p-nitrophenyl beta-D-ribofuranoside
-
-
0.09
phosphate
-
reaction with inosine, mutant enzyme N243A
1.1
phosphate
-
reaction with inosine, mutant enzyme K244A
1.5
phosphate
-
reaction with inosine, mutant enzyme R84A
1.8
phosphate
-
reaction with inosine, mutant enzyme E201A
2.01
phosphate
-
reaction with 7-methylinosine
3.5
phosphate
-
cosubstrate guanosine, pH 7.0, 25°C
4.5
phosphate
-
pH 7.5, 37°C
4.9
phosphate
-
cosubstrate 2'-deoxyguanosine, pH 7.0, 25°C
4.9
phosphate
-
cosubstrate inosine, pH 7.0, 25°C
6.2
phosphate
-
reaction with inosine, mutant enzyme F200A
6.9
phosphate
-
cosubstrate 2'-deoxyinosine, pH 7.0, 25°C
9.4
phosphate
-
reaction with inosine, mutant enzyme H86A
12
phosphate
-
reaction with inosine, mutant enzyme T242A
13
phosphate
-
reaction with inosine, mutant enzyme H257A
13.3
phosphate
-
cosubstrate 7-methylinosine, pH 7.0, 25°C
17.9
phosphate
-
cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25°C
27.8
phosphate
-
reaction with 7-methylinosine
30
phosphate
-
reaction with inosine, mutant enzyme F159A
41
phosphate
-
reaction with inosine, mutant enzyme M219A
52
phosphate
-
reaction with inosine, mutant enzyme K244A
53
phosphate
-
reaction with inosine, mutant enzyme E89A
56
phosphate
-
wild-type enzyme
56
phosphate
-
reaction with inosine, mutant enzyme Y88F
56
phosphate
recombinant erythrocyte enzyme
76
phosphate
pH 7.5, 37°C
4
Xanthosine
-
-
16.8
Xanthosine
recombinant wild-type enzyme, pH 7.0, 25°C
23.3
Xanthosine
recombinant mutant N256D, pH 7.0, 25°C
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
2.4.2.1 purine-nucleoside phosphorylase
results (
results (
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