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Literature summary for 2.4.2.1 extracted from
- Purification and properties of purine nucleoside phosphorylase from Salmonella typhimurium (1973), J. Biol. Chem., 248, 2040-2043.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.047 | - |
deoxyinosine | - |
Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 0.05 | - |
Inosine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.37 | - |
phosphate | reaction with inosine | Salmonella enterica subsp. enterica serovar Typhimurium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 1.65fold stimulation | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23500 | - |
6 * 23500, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 130000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
| 141000 | - |
equilibrium sedimentation | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
LT-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 160 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| deoxyadenosine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | alpha-D-ribose 1-phosphate + adenine | - |
? | |
| deoxyguanosine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyinosine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| guanosine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 23500, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
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Release 2023.1 (January 2023)
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