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Literature summary for 2.4.2.1 extracted from
- Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2 (1996), Biosci. Biotechnol. Biochem., 60, 1179-1180.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.18 | - |
Inosine | - |
Geobacillus stearothermophilus |  |
| 1.29 | - |
guanosine | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
3 * 30000, SDS-PAGE | Geobacillus stearothermophilus |
| 90000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
TH 6-2 | - |
| Geobacillus stearothermophilus TH 6-2 | - |
TH 6-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 91.6 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + phosphate | no activity | Geobacillus stearothermophilus | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| adenosine + phosphate | no activity | Geobacillus stearothermophilus TH 6-2 | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | 85% of the activity with inosine | Geobacillus stearothermophilus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | 85% of the activity with inosine | Geobacillus stearothermophilus TH 6-2 | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyinosine + phosphate | as active as inosine | Geobacillus stearothermophilus | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| deoxyinosine + phosphate | as active as inosine | Geobacillus stearothermophilus TH 6-2 | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| guanosine + phosphate | 84% of the activity with guanosine | Geobacillus stearothermophilus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | 84% of the activity with guanosine | Geobacillus stearothermophilus TH 6-2 | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Geobacillus stearothermophilus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Geobacillus stearothermophilus TH 6-2 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| xanthosine + phosphate | - |
Geobacillus stearothermophilus | alpha-D-ribose 1-phosphate + xanthine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 30000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable below | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Geobacillus stearothermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | stable from pH 5.0 to pH 9.0 | Geobacillus stearothermophilus |
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