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2.4.2.1: purine-nucleoside phosphorylase

This is an abbreviated version!
For detailed information about purine-nucleoside phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.1

Reaction

purine 2'-deoxyribonucleoside
+
phosphate
=
purine
 +
2-deoxy-alpha-D-ribose 1-phosphate

Synonyms

5'-deoxy-5'-methylthioadenosine phosphorylase II, 5'-methythioadenosine phosphorylase, adenine nucleoside phosphorylase, adenosine phosphorylase, AgPNP, AmPNP, ANP, ApMTAP, apPNP, bovPNP, bPNO, CKP1, cytokinin riboside phosphorylase, DeoD, deoD_1, DgPNP, EC592_06680, EcPNP, flB, GsPNPI, GsPNPII, GtPNP, hPNP, HpPNP, HsPNO, HsPNP, Ino-Guo phosphorylase, inosine phosphorylase, inosine-guanosine phosphorylase, KlacPNP, KLLA0_C16621g, MTAPII, MtbPNP, nucleotide phosphatase (2.4.2.1), PfPNP, phosphorylase, purine nucleoside, PiPNP, PNP, PNP 1, PNP 2, PNP I, PNP II, PNP-II, PNP-Zg, PNPase, pNPI, PNPII, Pu-NPase, punA, PUNP, PUNPI, PUNPII, purine deoxynucleoside phosphorylase, purine deoxyribonucleoside phosphorylase, purine nucleoside orthophosphate ribosyl transferase, purine nucleoside phosphorylase, purine nucleoside phosphorylase 1, purine nucleoside phosphorylase 2, purine nucleoside phosphorylase DeoD, purine nucleoside phosphorylase DeoD-type, purine nucleoside phosphorylase II, purine ribonucleoside phosphorylase, purine-nucleoside phosphorylases II, SalT, SSO2343, TgPNP, TTC0194, TTC1070, TtPNPI, TtPNPII, TvPNP, xanthosine phosphorylase, XAP, xapA, ylmD

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.1 purine-nucleoside phosphorylase

Storage Stability

Storage Stability on EC 2.4.2.1 - purine-nucleoside phosphorylase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50 mM Tris-HCl, 10 mM 2-mercaptoethanol, pH 7.0, stable for several months
-
-20°C, 50% loss of activity after 2 weeks
-
20°C, room temperature, stable for several days in concentrated solution, 1% in 0.02% sodium azide
-
25°C, phosphate buffer pH 7.0, 130 h, 30% loss of activity
30°C, 1 month, less than 20% loss of activity
-
38°C, phosphate buffer pH 7.0, 130 h, 24% loss of activity
4°C or frozen, stable for long periods of time
-
4°C, 10 mM sodium phosphate buffer, pH 7.0, stable for at least 4 months provided a weekly dialysis with 2 mM DTT is performed
-
4°C, enzyme containing 2-mercaptoethanol prior to precipitation with ammonium sulfate, near pH 7, retains maximal catalytic activity for several months when stored as a precipitate
-
4°C, pH 7.2, 10 mM Tris-HCl buffer, stable for up to 14 days
-
4°C, stable for 2 years
purified recombinant His-tagged enzyme at 25 mg/ml is stable for months when stored at 8°C in an elution buffer containing 25 mM Tris-HCl, 100 mM NaCl, 10% glycerol, pH 7.0, up to 65% activity is retained after storage under these conditions for over 30 days
storage of the purified enzyme at 4°C for one month at 0.4 mg/ml in 100 mM Tris-HCl, pH 7.6, results in an about 50% decrease in activity