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4.2.1.11: phosphopyruvate hydratase

This is an abbreviated version!
For detailed information about phosphopyruvate hydratase, go to the full flat file.

Word Map on EC 4.2.1.11

Reaction

2-phospho-D-glycerate
=
phosphoenolpyruvate
 +
H2O

Synonyms

14-3-2 protein, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydrolase, 2-phospho-D-glycerate hydrolyase, 2-phospho-D-glyceratehydrolyase, 2-phosphoglycerate dehydratase, 2-phosphoglycerate enolase, 2-phosphoglycerate hydrolase, 2-phosphoglycerate hydrolyase, 2-phosphoglyceric dehydratase, 2-phosphopyruvate hydrolyase, alpha,alpha-enolase, alpha-enolase, alpha-enolase 1, alpha-enolase 2, Alt a XI, beta,beta-enolase, beta-enolase, Cla h VI, Csenolase, cytotoxin B, EhENO, ENO, ENO-1, Eno-alpha, ENO-S, ENO1, Eno1p, Eno2, ENOA, EnoA I, EnoA1, ENOL, Enol-1, enolase, enolase 1, enolase 2, enolase alpha, enolase gamma, enolase S, enolase-1, enolase-alpha, enolase-beta, Enolase-phosphatase E1, enolase2p, Err2p, Err3p, gamma,gamma-enolase, gamma-enolase, hENO1, HLE1, human alpha-enolase, human neuron-specific enolase, hydratase, phosphoenolpyruvate, Laminin binding protein, Major allergen Alt a 11, MASA, MSE, Neural enolase, neuron specific enolase, neuron-specific enolase, neuronal enolase, NNE, Non-neural enolase, NSE, OSE1, p45, P46, Pfen, Pfeno, phosphoenolpyruvate enolase, phosphoenolpyruvate hydratase, Phosphopyruvate hydratase, plasminogen-binding alpha-enolase, R-NSE, r-Pfen, rBaEn, rSsEno, SEN, SjENO, Skeletal muscle enolase, SPM2, SsEno, SSO0913, streptococcal surface enolase, Tau-crystallin, tv-ENO1, tv-rENO1, XEP, yeast enolase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.11 phosphopyruvate hydratase

Cloned

Cloned on EC 4.2.1.11 - phosphopyruvate hydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ability of Pfeno to complement a mutant Saccharomyces cervisiae strain R11258 deficient in enolase activity. In this strain Tetr-Eno2, the enolase 1 gene is deleted and expression of the enolase 2 gene is under the control of a tetracycline repressible promoter. Pfeno is able to restore all three phenotypic effects fully or partially, i.e. growth retardation, vacuolar fragmentation and altered expression of certain vacuolar proteins, overview
-
cDNA inserted in PGEX-4T-3 expressed in Escherichia coli, glutathione S-transferase fusion protein, protein with 436 amino acid residues, 70-80% sequence similarity to enolases from other organisms, including helminth parasites
clone Eg_PSGRS_13B09, cloning from cDNA library, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) in inclusion bodies
-
co-expression of enolase 1 in bone marrow-derived mast cells with calreticulin
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21 Star(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS using the pRSETA expression vector, recombinant His-tagged protein
expressed in Escherichia coli BL21, pET17b expression vector
expressed in Escherichia coli host strain M15-pREP4, His-tagged protein
expressed in Escherichia coli Rosetta (DE3) cells
-
expressed in Escherichia coli Rosetta (DE3)pLysS cells
-
expressed in Escherichia coli Rosetta cells
-
expressed in Escherichia coli Rosettagami (DE3) cells
expressed in Escherichia coli strains M15 and SG13009 using expression vector pQE30, protein of 431 amino acids identified, homologies to other trematode enolases estimated
expressed in Escherichia coli, expression vector pQE30, His-tagged fusion protein
expressed in Escherichia coli, His-tagged recombinant protein
expressed in Escherichia coli, His-tagging and GST-tagging of recombinant enolase protein
expressed in Escherichia coli, PCR-amplified genomic DNA from Bacillus subtilis strain 168 ligated into pET15b encoding an N-terminal six-His tag vector
expression in Escherichia coli
expression in Escherichia coli of a his-tagged recombinant enzyme
expression in Escherichia coli strain BL21 AI with an N-terminal 10His-tag
expression in Escherichia coli strain DH5alpha with His-tag, recombinant enzyme without enolase activity, gaines activity after cutting off the signal peptide from the full-length protein
-
expression in Escherichia coli strain JM109 with C-terminal His-tag
-
expression in Escherichia coli strain XL1-Blue and BL-21 with N-terminal His-tag
-
expression in Escherichia coli strain XL1Blue as an N-terminal His6-tagged protein
-
expression of GST-tagged ENO in Escherichia coli BL21 (DE3)
expression of GST-tagged enolase in Escherichia coli
expression of His-tagged alpha-enolase in Escherichia coli strain BL21(DE3)
-
expression of His-tagged enolase in Escherichia coli strain BL21
expression of recombinant wild-type and mutant enzymes in Escherichia coli
expression of the mutants E211Q and E168Q in Escherichia coli
expression of the recombinant H159A and H159G mutants in Escherichia coli
-
expression of wild-type and mutant genes in Escherichia coli
-
gamma-enolase
-
gene LOS, DNA and amino acid sequence determination and analysis, semi-quantitative RT-PCR expression analysis, overview
identified from cDNA library, expression in Escherichia coli
inserted into pET-3a, expressed in Escherichia coli BL21-DE3, site-directed mutagenesis performed
isolated from a genomic library, over-expressed in Escherichia coli, expression vector pET28a
isolated from cDNA library, expressed in Escherichia coli BL21, expression vector pET22b, 60% sequence similarities to human non-neuronal alpha enolase, conserved residues determined
neuron specific enolase and modified neuron-specific enolases: Y-NSE with one Tyr residue added at the N-terminal of the recombinant neuron-specific enolase
-
over-expression in Escherichia coli strain BL21, expression vector pET3a, His-tagged recombinant protein
-
recombinant enolase, expressed in Escherichia coli BL21-DE3-pLysS strain harbouring the recombinant pET28a plasmid
recombinant protein expressed as a glutathione S-transferase GST::tv-ENO1 fusion protein in Escherichia coli, cleaved using thrombin
-
subcloned in Escherichia coli
the three genes, ENO1, ENO2 and ENO3, encoding for three isoforms of the enzyme, alpha-enolase, gamma-enolase, and beta-enolase, respectively, show high sequence identity
-