Literature summary for 4.2.1.11 extracted from

Sijbrandi, R.; Den Blaauwen, T.; Tame, J.R.; Oudega, B.; Luirink, J.; Otto, B.R.
Characterization of an iron-regulated alpha-enolase of Bacteroides fragilis (2005), Microbes Infect., 7, 9-18.

Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21 AI with an N-terminal 10His-tag	Bacteroides fragilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.21	-	2-phospho-D-glycerate	37°C, in reaction buffer	Bacteroides fragilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
inner membrane	-	Bacteroides fragilis	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	-	Bacteroides fragilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49000	-	SDS-PAGE	Bacteroides fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-D-glycerate	Bacteroides fragilis	-	phosphoenolpyruvate + H2O	-	r
2-phospho-D-glycerate	Bacteroides fragilis YCH46	-	phosphoenolpyruvate + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Bacteroides fragilis	Q8KNX9	strain BE1	-
Bacteroides fragilis YCH46	Q8KNX9	strain BE1	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-charged HiTrap chelating column	Bacteroides fragilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Bacteroides fragilis	phosphoenolpyruvate + H2O	-	r
2-phospho-D-glycerate	-	Bacteroides fragilis YCH46	phosphoenolpyruvate + H2O	-	r

Synonyms

Synonyms	Comment	Organism
P46	-	Bacteroides fragilis

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Release 2023.1 (January 2023)