Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
neuron specific enolase and modified neuron-specific enolases: Y-NSE with one Tyr residue added at the N-terminal of the recombinant neuron-specific enolase | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant neuron-specific enolase (R-NSE) has markedly decreased binding affinity to anti-neuron-specific enolase antibodies. Reactivity of modified neuron-specific enolases (Y-NSE with one Tyr residue added at the N-terminal of the recombinant neuron-specific enolase. Y-NSE.H6 with six His residues further added at the C-terminal of recombinant neuron-specific enolase) to the antibody is almost equivalent to that of human brain gamma,gamma-enolase | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
2-phospho-D-glycerate | Y-NSE.H6 | Homo sapiens | |
0.24 | - |
2-phospho-D-glycerate | R-NSE | Homo sapiens | |
0.25 | - |
2-phospho-D-glycerate | Y-NSE | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, enzyme R-NSE, enzyme Y-NSE, SDS-PAGE | Homo sapiens |
47000 | - |
x * 47000, Y-NSE.H6, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Homo sapiens | phosphoenolpyruvate | - |
? | |
2-phospho-D-glycerate | - |
Homo sapiens | phosphoenolpyruvate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, enzyme R-NSE, enzyme Y-NSE, SDS-PAGE | Homo sapiens |
? | x * 47000, Y-NSE.H6, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.6 | R-NSE, Y-NSE, Y-NSE.H6 | Homo sapiens |