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Literature summary for 4.2.1.11 extracted from

  • Kaneta, M.; Aoki, T.; Onagi, H.; Morikawa, J.; Watabe, H.
    Biochemical and immunochemical properties of modified human neuron-specific enolase (1997), Biol. Pharm. Bull., 20, 556-559.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens
neuron specific enolase and modified neuron-specific enolases: Y-NSE with one Tyr residue added at the N-terminal of the recombinant neuron-specific enolase Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information recombinant neuron-specific enolase (R-NSE) has markedly decreased binding affinity to anti-neuron-specific enolase antibodies. Reactivity of modified neuron-specific enolases (Y-NSE with one Tyr residue added at the N-terminal of the recombinant neuron-specific enolase. Y-NSE.H6 with six His residues further added at the C-terminal of recombinant neuron-specific enolase) to the antibody is almost equivalent to that of human brain gamma,gamma-enolase Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
2-phospho-D-glycerate Y-NSE.H6 Homo sapiens
0.24
-
2-phospho-D-glycerate R-NSE Homo sapiens
0.25
-
2-phospho-D-glycerate Y-NSE Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
x * 46000, enzyme R-NSE, enzyme Y-NSE, SDS-PAGE Homo sapiens
47000
-
x * 47000, Y-NSE.H6, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Homo sapiens phosphoenolpyruvate
-
?
2-phospho-D-glycerate
-
Homo sapiens phosphoenolpyruvate + H2O
-
?

Subunits

Subunits Comment Organism
? x * 46000, enzyme R-NSE, enzyme Y-NSE, SDS-PAGE Homo sapiens
? x * 47000, Y-NSE.H6, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.6 R-NSE, Y-NSE, Y-NSE.H6 Homo sapiens