Literature summary for 4.2.1.11 extracted from

Ehinger, S.; Schubert, W.D.; Bergmann, S.; Hammerschmidt, S.; Heinz, D.W.
Plasmin(ogen)-binding alpha-enolase from Streptococcus pneumoniae: crystal structure and evaluation of plasmin(ogen)-binding sites (2004), J. Mol. Biol., 343, 997-1005.

Search for more literature for 4.2.1.11

Application

Application	Comment	Organism
molecular biology	alpha-enolase doubles as a surface-displayed plasminogen-binder supporting virulence	Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment)	Organism
2.0 A resolution, molecular replacement, hanging-drop vapor-diffusion	Streptococcus pneumoniae

Protein Variants

Protein Variants	Comment	Organism
deltaK433/K434	mutant with different oligomerization state	Streptococcus pneumoniae
K433L/K434L	mutant with different oligomerization state	Streptococcus pneumoniae
K434L	mutant with different oligomerization state	Streptococcus pneumoniae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
370000	-	gel filtration	Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-D-glycerate	Streptococcus pneumoniae	-	phosphoenolpyruvate + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	Q97QS2	-	-

Purification (Commentary)

Purification (Comment)	Organism
ion exchange chromatography and gel filtration	Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
111	-	room temperature, pH 7.5, MgCl2, KCl, 2-phosphoglyceric acid	Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Streptococcus pneumoniae	phosphoenolpyruvate + H2O	-	r

Subunits

Subunits	Comment	Organism
octamer	-	Streptococcus pneumoniae

Synonyms

Synonyms	Comment	Organism
plasminogen-binding alpha-enolase	-	Streptococcus pneumoniae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)