Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
engineered K345A/N80D/N126D heterodimer in complex with substrate/product, 1.85A resolution, batch method | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E211Q | inactive, but properly folded | Saccharomyces cerevisiae |
K345A | inactive | Saccharomyces cerevisiae |
K345A/N80D/N126D | heterodimer with one inactive K345A subunit and one active N80D and N126D subunit | Saccharomyces cerevisiae |
N80D/N126D | mutant with surface mutations to facilitate ion-exchange chromatographic separation | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
2-phospho-D-glycerate | wild type, 25°C, Mg2+, pH 7.5 | Saccharomyces cerevisiae | |
0.05 | - |
2-phospho-D-glycerate | K345A/N80D/N126D, 25°C, Mg2+, pH 7.5 | Saccharomyces cerevisiae | |
0.057 | - |
2-phospho-D-glycerate | N80D/N126D, 25°C, Mg2+, pH 7.5 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Saccharomyces cerevisiae | - |
phosphoenolpyruvate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P00924 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Saccharomyces cerevisiae | phosphoenolpyruvate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
heterodimer | engineered type | Saccharomyces cerevisiae |
homodimer | N80D/N126D | Saccharomyces cerevisiae |