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Literature summary for 4.2.1.11 extracted from

  • Gan, W.; Zhao, G.; Xu, H.; Wu, W.; Du, W.; Huang, J.; Yu, X.; Hu, X.
    Reverse vaccinology approach identify an Echinococcus granulosus tegumental membrane protein enolase as vaccine candidate (2010), Parasitol. Res., 106, 873-882.
    View publication on PubMed

Application

Application Comment Organism
medicine the tegumental membrane protein enolase is a vaccine candidate Echinococcus granulosus

Cloned(Commentary)

Cloned (Comment) Organism
clone Eg_PSGRS_13B09, cloning from cDNA library, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) in inclusion bodies Echinococcus granulosus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Echinococcus granulosus 16020
-

Organism

Organism UniProt Comment Textmining
Echinococcus granulosus
-
isolated from sheep in Qinghai province of China
-

Renatured (Commentary)

Renatured (Comment) Organism
solubilization of the recombinant enzyme from Escherichia coli inclusion bodies by 6 M urea Echinococcus granulosus

Source Tissue

Source Tissue Comment Organism Textmining
integument
-
Echinococcus granulosus
-

Subunits

Subunits Comment Organism
? x * 45000-66200, recombinant solubilized enzyme, SDS-PAGE Echinococcus granulosus
More the protein contains a potential nuclear localization sequence aa190-199 and several linear B cell epitopes and CTL T cell epitopes, of which the outside epitope aa49-57 and inside epitope aa228-236 are facultative T cell and B cell epitope, and the linear B cell epitope aa206-213 contains the active center site Glu210, suggesting the putative protein is a potential membrane with strong immunogenicity Echinococcus granulosus

Synonyms

Synonyms Comment Organism
enolase
-
Echinococcus granulosus