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Literature summary for 4.2.1.11 extracted from

  • Vastano, V.; Capri, U.; Candela, M.; Siciliano, R.A.; Russo, L.; Renda, M.; Sacco, M.
    Identification of binding sites of Lactobacillus plantarum enolase involved in the interaction with human plasminogen (2013), Microbiol. Res., 168, 65-72.
    View publication on PubMed
Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Lactiplantibacillus plantarum

Protein Variants

Protein Variants Comment Organism
K255A while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme Lactiplantibacillus plantarum
K259A K255A while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme Lactiplantibacillus plantarum
K259A/K422A K255A while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme Lactiplantibacillus plantarum
K422A K255A while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme Lactiplantibacillus plantarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, MALDI-TOF mass spectrometry Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Lactiplantibacillus plantarum
-
 phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate Lactiplantibacillus plantarum LM3
-
 phosphoenolpyruvate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum LM3
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Lactiplantibacillus plantarum phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate
-
 Lactiplantibacillus plantarum LM3 phosphoenolpyruvate + H2O
-
 ?
additional information the enzyme binds plasminogen Lactiplantibacillus plantarum ?
-
 ?
additional information the enzyme binds plasminogen Lactiplantibacillus plantarum LM3 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, MALDI-TOF mass spectrometry Lactiplantibacillus plantarum

Synonyms

Synonyms Comment Organism
EnoA1
-
 Lactiplantibacillus plantarum
enolase
-
 Lactiplantibacillus plantarum

pI Value

Organism Comment pI Value Maximum pI Value
Lactiplantibacillus plantarum isoelectric focusing
-
 4.6