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Literature summary for 4.2.1.11 extracted from

  • Prabhakar, V.; Loettgert, T.; Gigolashvili, T.; Bell, K.; Fluegge, U.I.; Haeusler, R.E.
    Molecular and functional characterization of the plastid-localized phosphoenolpyruvate enolase (ENO1) from Arabidopsis thaliana (2009), FEBS Lett., 583, 983-991.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.082
-
2-phospho-D-glycerate at pH 8.0 Arabidopsis thaliana
0.149
-
2-phospho-D-glycerate at pH 7.0 Arabidopsis thaliana
0.18
-
phosphoenolpyruvate at pH 8.0 Arabidopsis thaliana
0.39
-
3-phospho-D-glycerate at pH 7.0 Arabidopsis thaliana
0.534
-
phosphoenolpyruvate at pH 7.0 Arabidopsis thaliana
1.281
-
3-phospho-D-glycerate at pH 8.0 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
Arabidopsis thaliana 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51500
-
calculated from amino acid sequence Arabidopsis thaliana
61200
-
estimated from SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
additional information ENO1 is expressed in most heterotrophic tissues but not in the mesophyll of leaves Arabidopsis thaliana
-
non-root-hair cell
-
Arabidopsis thaliana
-
trichome
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Arabidopsis thaliana phosphoenolpyruvate + H2O
-
r
3-phospho-D-glycerate
-
Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
ENO1
-
Arabidopsis thaliana
phosphoenolpyruvate enolase
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
in the direction of 2-phospho-D-glycerate formation Arabidopsis thaliana
8
-
in the direction of phosphoenolpyruvate formation Arabidopsis thaliana