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4.1.2.13: fructose-bisphosphate aldolase

This is an abbreviated version!
For detailed information about fructose-bisphosphate aldolase, go to the full flat file.

Word Map on EC 4.1.2.13

Reaction

D-fructose 1,6-bisphosphate
=
glycerone phosphate
 +
D-glyceraldehyde 3-phosphate

Synonyms

1,6-Diphosphofructose aldolase, 37 kDa major allergen, 41 kDa antigen, ALD, ALDC, aldoA, Aldob, aldolase, aldolase A, aldolase B, aldolase C, aldolase, fructose diphosphate, ALDP, bifunctional fructose-1,6-bisphosphate aldolase/phosphatase, Brain-type aldolase, Ca-FBA-II, CE1, CE2, class I Fba, class I fructose 1,6-bisphosphate aldolase, class I fructose bisphosphate aldolase, class I fructose-1,6-bisphosphate aldolase, class I muscle fructose bis-phosphate aldolase, class II aldolase, class II FBA, class II FBP aldolase, class II fructose 1,6-bisphosphate aldolase, class II fructose bisphosphate aldolase, class II fructose-1,6-bisphosphate aldolase, class IIa fructose 1,6-bisphosphate aldolase, class IIb fructose 1,6-bisphosphate aldolase, ClassII FBP-aldolase, CoFBA1, CoFBA2, CoFBA3, CoFBA4, D-fructose-1,6-bisphosphate aldolase, D-fructose-1,6-bisphosphate D-glyceraldehyde-3-P-lyase, D-fructose-6-phosphate aldolase, Diphosphofructose aldolase, DLF, EC 4.1.2.7, F1,6-P2 aldolase, F1,6P2 aldolase, F16BP aldolase, FBA, FBA class II, FBA-II, Fba1, Fba1p, Fba2, FBA7, FbaA, FbaB, FbaC, FBAld, FbaP, FBP aldolase, FBP aldolase/phosphatase, FBP-aldolase, FBPA, FBPA I, FBPA-1, FBPA/P, FDP aldolase, FPA, Fru-1,6-P2 aldolase, Fru-P2A, Fructoaldolase, fructose 1,6 bisphosphate aldolase, fructose 1,6-bisphosphatase/aldolase, Fructose 1,6-bisphosphate aldolase, fructose 1,6-bisphosphate aldolase/phosphatase, Fructose 1,6-diphosphate aldolase, Fructose 1-monophosphate aldolase, Fructose 1-phosphate aldolase, fructose bis-phosphate aldolase, Fructose bisphosphate aldolase, Fructose diphosphate aldolase, fructose-1,6-biphosphate aldolase, fructose-1,6-bisphosphate (FBP) aldolase/phosphatase, fructose-1,6-bisphosphate aldolase, fructose-1,6-bisphosphate aldolase A, fructose-1,6-bisphosphate aldolase B, fructose-1,6-bisphosphate aldolase class II, fructose-1,6-bisphosphate aldolase/phosphatase, fructose-1,6-bisphosphate muscle aldolase, Fructose-1,6-bisphosphate triosephosphate-lyase, fructose-bisphosphate aldolase, fructose-bisphosphate aldolase A, FSA, fuculose aldolase, heat-induced protein 44, HIP44, IgE-binding allergen, ketose 1-phosphate aldolase, Leishmania aldolase, Liver-type aldolase, MGA3_01355, MJ0400-His6, More, MtFBA, Muscle-type aldolase, Ov-fba-1, OvFBPA-1, Pcal_0111, Phosphofructoaldolase, SMALDO, SSO0286, STK_03180, TgALD1, Tneu_0133, TnFBPAP, Tt-FBPA, zerebrin II, zymohexase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.13 fructose-bisphosphate aldolase

Temperature Stability

Temperature Stability on EC 4.1.2.13 - fructose-bisphosphate aldolase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
enzyme from patients with fructose intolerance shows increased temperature sensitivity, activity at 10°C is 6fold lower than that of the wild-type, and activity decreases substantially at higher temperatures
120
-
20 min, about 20% loss of activity
130
-
10 min, complete inactivation
25
-
3 d, 25% loss of activity
30
-
pH 10, 15 min, 5% loss of activity
30 - 55
activity lost substantially at higher temperature
30 - 80
the enzyme activity is retained after incubation for 2 h at 30, 40, or 50°C, respectively. The half-life of the enzyme is 1 h at 60°C, and the enzyme is fully inactivated after heating at 80°C for 10 min
40
W147R melting temperature of W147R mutant and L256P mutant is 40°C versus 47°C for the wild-type enzyme
45
-
stable for 60 min
45 - 60
-
the aldolase loses 25% of its activity after 10 min of incubation at 45°C and loses almost all its activity after a10 min incubation at 60°C
47
melting temperature of the wild-type enzyme
48
melting temperature of mutant E206K
51
50% loss of activity after incubation for 10 min at 50.5°C
54
melting temperature of wild-type enzyme is 54.4°C
55 - 70
-
the enzyme retains almost 100% activity after incubation at 55°C for 12 h, while enzyme activity is about 10% after incubation at 70°C for 12
56
-
aldolase activity, which is lost within 2 min at 56°C, can be retained for 30 min at the same temperature in the presence of 100 mM phosphate
90 - 100
purified recombinant enzyme, half-life is 120 min, no significant change in the circular dichroism spectra of the protein up to 90°C
90 - 93
-
half-life: 2 h
95
-
half-life: 46 min
additional information