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0.0002 - 64.5
D-fructose 1,6-bisphosphate
0.008 - 6.08
D-Fructose 1-phosphate
0.01 - 12
D-fructose-1,6-bisphosphate
0.002 - 12.6
D-glyceraldehyde 3-phosphate
0.002
dihydroxyacetone phosphate
in the absence of Co2+, at pH 7.5, 30°C
0.167 - 10.8
fructose 1-phosphate
0.1 - 8
glycerone phosphate
0.003
sedoheptulose 1,7-bisphosphate
in the absence of Co2+, at pH 7.5, 30°C
25.2
sedoheptulose 1,7-diphosphate
-
-
33.3
sedoheptulose 7-phosphate
-
pH 7.5, 30°C
additional information
additional information
-
0.0002
D-fructose 1,6-bisphosphate
turnover number less than 0.0002 sec-1, mutant enzyme K146A
0.001
D-fructose 1,6-bisphosphate
30°C, N286D mutant
0.0019
D-fructose 1,6-bisphosphate
mutant enzyme K146M
0.002
D-fructose 1,6-bisphosphate
mutant enzyme D33S
0.0028
D-fructose 1,6-bisphosphate
30°C, D109A mutant
0.003
D-fructose 1,6-bisphosphate
mutant enzyme D33N
0.005
D-fructose 1,6-bisphosphate
mutant enzyme E187Q
0.005
D-fructose 1,6-bisphosphate
in the absence of Co2+, at pH 7.5, 30°C
0.011
D-fructose 1,6-bisphosphate
-
pH 7.4, 30°C, enzyme from patients with intolerance
0.013
D-fructose 1,6-bisphosphate
-
E174A mutant, 30°C
0.018
D-fructose 1,6-bisphosphate
in the presence of 0.02 mM Co2+, at substrate concentrations of 0.008-0.5 mM, at pH 7.5, 30°C
0.026
D-fructose 1,6-bisphosphate
pH 8.0, 48°C, mutant enzyme Y348F
0.027
D-fructose 1,6-bisphosphate
pH 8.0, 48°C, wild-type enzyme
0.029
D-fructose 1,6-bisphosphate
-
pH 7.4, 10°C, enzyme from patients with intolerance
0.03
D-fructose 1,6-bisphosphate
E187Q mutant, pH 7.6, 22°C
0.033
D-fructose 1,6-bisphosphate
-
E182A mutant, 30°C
0.046
D-fructose 1,6-bisphosphate
mutant enzyme E187A
0.055
D-fructose 1,6-bisphosphate
in the presence of 0.02 mM Co2+, at substrate concentrations of 0.5-1.5 mM, at pH 7.5, 30°C
0.12
D-fructose 1,6-bisphosphate
E187A mutant, pH 7.6, 22°C
0.16
D-fructose 1,6-bisphosphate
-
N35A mutant, 30°C
0.19
D-fructose 1,6-bisphosphate
30°C, N286A mutant
0.19
D-fructose 1,6-bisphosphate
N334K mutant, 22°C
0.19
D-fructose 1,6-bisphosphate
-
pH 7.4, 10°C, wild-type enzyme
0.43
D-fructose 1,6-bisphosphate
W147R mutant, 22°C
0.54
D-fructose 1,6-bisphosphate
L256P mutant, 22°C
0.57
D-fructose 1,6-bisphosphate
Q354E mutant, 22°C
0.58
D-fructose 1,6-bisphosphate
-
K325A mutant, 30°C
0.61
D-fructose 1,6-bisphosphate
wild-type enzyme, 22°C
0.63
D-fructose 1,6-bisphosphate
mutant enzyme D255A, in 50 mM K+-HEPES (pH 7.5), at 25°C
0.65
D-fructose 1,6-bisphosphate
mutant enzyme K107M
0.85
D-fructose 1,6-bisphosphate
-
S61A mutant, 30°C
1.07
D-fructose 1,6-bisphosphate
30°C, AB_All mutant
1.16
D-fructose 1,6-bisphosphate
at pH 7.8 and 25°C
1.16
D-fructose 1,6-bisphosphate
pH 7.8, temperature not specified in the publication
1.53
D-fructose 1,6-bisphosphate
30°C, aldolase B
1.53
D-fructose 1,6-bisphosphate
-
in Tris-HCl pH 8.0, at 30°C
1.6
D-fructose 1,6-bisphosphate
pH 7.8, 55°C
1.63
D-fructose 1,6-bisphosphate
-
pH 5.5
1.69
D-fructose 1,6-bisphosphate
E189Q mutant, pH 7.6, 22°C
2
D-fructose 1,6-bisphosphate
30°C, D144A mutant
2.1
D-fructose 1,6-bisphosphate
-
pH 7.4, 30°C, wild-type enzyme
2.1
D-fructose 1,6-bisphosphate
-
at 30°C in 50 mM Tris-HCl, 0.1 M potassium acetate buffer (pH 8.0)
2.2
D-fructose 1,6-bisphosphate
30°C, D288A mutant
2.83
D-fructose 1,6-bisphosphate
-
mutant aldolase C Y363S
2.94
D-fructose 1,6-bisphosphate
30°C, aldolase B
2.94
D-fructose 1,6-bisphosphate
E189Q mutant, pH 7.6, 22°C
2.95
D-fructose 1,6-bisphosphate
-
in Tris-HCl pH 8.0, at 30°C
3.4
D-fructose 1,6-bisphosphate
E189A mutant, pH 7.6, 22°C
3.55
D-fructose 1,6-bisphosphate
-
at pH 7.5 and 25°C, in the absence of Zn2+
3.55
D-fructose 1,6-bisphosphate
wild type enzyme, in 50 mM K+-HEPES (pH 7.5), at 25°C
4.02
D-fructose 1,6-bisphosphate
-
pH 9.3
4.7
D-fructose 1,6-bisphosphate
mutant enzyme G346S
5.03
D-fructose 1,6-bisphosphate
-
pH 5.5
5.1
D-fructose 1,6-bisphosphate
pH 7.6, 50°C
5.2
D-fructose 1,6-bisphosphate
-
-
5.28
D-fructose 1,6-bisphosphate
-
pH 9.2
5.47
D-fructose 1,6-bisphosphate
-
pH 7.6
5.8
D-fructose 1,6-bisphosphate
-
E181A mutant, 30°C
6.08
D-fructose 1,6-bisphosphate
30°C, AB_All mutant
6.08
D-fructose 1,6-bisphosphate
-
K325A mutant, 30°C
6.08
D-fructose 1,6-bisphosphate
L256P mutant, 22°C
6.08
D-fructose 1,6-bisphosphate
Q354E mutant, 22°C
6.08
D-fructose 1,6-bisphosphate
wild-type enzyme, 22°C
6.3
D-fructose 1,6-bisphosphate
-
S61T mutant, 30°C
7
D-fructose 1,6-bisphosphate
30°C, D290A mutant
7.09
D-fructose 1,6-bisphosphate
pH 7.5, 25°C
7.09
D-fructose 1,6-bisphosphate
at pH 7.5 and 25°C
7.5
D-fructose 1,6-bisphosphate
-
pH 9.3
7.87
D-fructose 1,6-bisphosphate
-
pH 5.5
8.2
D-fructose 1,6-bisphosphate
pH 7.6
8.2
D-fructose 1,6-bisphosphate
30°C, wild-type enzyme
8.5
D-fructose 1,6-bisphosphate
-
Q59A mutant, 30°C
10.2
D-fructose 1,6-bisphosphate
at 30°C
10.2
D-fructose 1,6-bisphosphate
30°C, aldolase A
10.3
D-fructose 1,6-bisphosphate
-
pH 7.6, native enzyme
10.5
D-fructose 1,6-bisphosphate
-
wild-type enzyme, 30°C
10.7
D-fructose 1,6-bisphosphate
mutant enzyme E206K
10.9
D-fructose 1,6-bisphosphate
-
pH 7.6, recombinant enzyme
12.3
D-fructose 1,6-bisphosphate
30°C, D329A mutant
12.6
D-fructose 1,6-bisphosphate
-
aldolase C
13
D-fructose 1,6-bisphosphate
-
30°C
13.22
D-fructose 1,6-bisphosphate
native wild type enzyme
13.9
D-fructose 1,6-bisphosphate
-
pH 7.6
14
D-fructose 1,6-bisphosphate
-
wild-type and mutants, pH 7.4
14.2
D-fructose 1,6-bisphosphate
30°C
15.8
D-fructose 1,6-bisphosphate
-
non-muscle-type enzyme
16.5
D-fructose 1,6-bisphosphate
30°C, H156E mutant
16.7
D-fructose 1,6-bisphosphate
wild-type enzyme
16.76
D-fructose 1,6-bisphosphate
-
native enzyme, in Tris-HCl buffer (40 mM, pH 8.0), at 25°C
17.9
D-fructose 1,6-bisphosphate
-
recombinant non-muscle-type enzyme
20.1
D-fructose 1,6-bisphosphate
-
aldolase B
20.3
D-fructose 1,6-bisphosphate
-
recombinant fusion protein DLF, in Tris-HCl buffer (40 mM, pH 8.0), at 25°C
21
D-fructose 1,6-bisphosphate
pH 7.3, 28°C
30.1
D-fructose 1,6-bisphosphate
-
in Tris-HCl pH 8.0, at 30°C
33
D-fructose 1,6-bisphosphate
-
aldolase C
36.9
D-fructose 1,6-bisphosphate
E189A mutant, pH 7.6, 22°C
40
D-fructose 1,6-bisphosphate
-
pH 7.5, 30°C
42.2
D-fructose 1,6-bisphosphate
native recombinant enzyme, pH 7.6, 22°C
45.7
D-fructose 1,6-bisphosphate
-
in Tris-HCl pH 8.0, at 30°C
45.9
D-fructose 1,6-bisphosphate
-
-
46
D-fructose 1,6-bisphosphate
-
at pH 7.8
46.1
D-fructose 1,6-bisphosphate
-
muscle-type enzyme
49.3
D-fructose 1,6-bisphosphate
-
recombinant muscle-type enzyme
59.7
D-fructose 1,6-bisphosphate
-
aldolase A
64.5
D-fructose 1,6-bisphosphate
-
-
0.008
D-Fructose 1-phosphate
-
pH 7.4, 30°C, enzyme from patients with intolerance
0.019
D-Fructose 1-phosphate
-
pH 7.4, 10°C, enzyme from patients with intolerance
0.059
D-Fructose 1-phosphate
Q354E mutant, 22°C
0.085
D-Fructose 1-phosphate
N334K mutant, 22°C
0.12
D-Fructose 1-phosphate
-
pH 7.4, 10°C, wild-type enzyme
0.25
D-Fructose 1-phosphate
W147R mutant, 22°C
0.38
D-Fructose 1-phosphate
L256P mutant, 22°C
0.63
D-Fructose 1-phosphate
at 30°C
0.63
D-Fructose 1-phosphate
30°C, aldolase A
0.67
D-Fructose 1-phosphate
wild-type enzyme, 22°C
1.47
D-Fructose 1-phosphate
30°C, AB_All mutant
2
D-Fructose 1-phosphate
-
pH 7.4, 30°C, wild-type enzyme
2.8
D-Fructose 1-phosphate
-
-
6.08
D-Fructose 1-phosphate
30°C, AB_All mutant
6.08
D-Fructose 1-phosphate
30°C, aldolase A
6.08
D-Fructose 1-phosphate
30°C, aldolase B
6.08
D-Fructose 1-phosphate
wild-type enzyme, 22°C
0.01
D-fructose-1,6-bisphosphate
mutant E169A, pH 7.8, 22°C
0.11
D-fructose-1,6-bisphosphate
mutant G167A/G166A, pH 7.8, 22°C
0.17
D-fructose-1,6-bisphosphate
mutant G167A, pH 7.8, 22°C
0.45
D-fructose-1,6-bisphosphate
mutant D276A, pH 7.8, 22°C
2.67
D-fructose-1,6-bisphosphate
mutant E168A, pH 7.8, 22°C
12
D-fructose-1,6-bisphosphate
wild-type, pH 7.8, 22°C
0.002
D-glyceraldehyde 3-phosphate
in the absence of Co2+, at pH 7.5, 30°C
0.1
D-glyceraldehyde 3-phosphate
pH 8.0, 48°C, mutant enzyme Y348F
0.56
D-glyceraldehyde 3-phosphate
pH 7.6, 50°C
0.91
D-glyceraldehyde 3-phosphate
pH 8.0, 48°C, wild-type enzyme
12.6
D-glyceraldehyde 3-phosphate
pH 7.8, 55°C
0.167
fructose 1-phosphate
-
pH 9.3
0.183
fructose 1-phosphate
-
pH 5.5
0.267
fructose 1-phosphate
-
pH 7.6
0.45
fructose 1-phosphate
-
pH 5.5
0.583
fructose 1-phosphate
-
pH 9.3
0.75
fructose 1-phosphate
-
mutant aldolase C Y363S
1.05
fructose 1-phosphate
-
pH 7.6
1.13
fructose 1-phosphate
-
pH 5.5
1.27
fructose 1-phosphate
-
pH 9.2
1.28
fructose 1-phosphate
-
aldolase A
1.38
fructose 1-phosphate
-
muscle-type enzyme
1.48
fructose 1-phosphate
-
aldolase C
1.55
fructose 1-phosphate
-
recombinant muscle-type enzyme
1.7
fructose 1-phosphate
-
pH 7.6
2.67
fructose 1-phosphate
-
aldolase C
3.6
fructose 1-phosphate
-
non-muscle-type enzyme
3.65
fructose 1-phosphate
-
recombinant non-muscle-type enzyme
10.8
fructose 1-phosphate
-
aldolase B
0.1
glycerone phosphate
pH 8.0, 48°C, mutant enzyme Y348F
0.4
glycerone phosphate
pH 7.6, 50°C
0.91
glycerone phosphate
pH 8.0, 48°C, wild-type enzyme
8
glycerone phosphate
pH 7.8, 55°C
additional information
additional information
-
-
-
additional information
additional information
-
turnover-numbers of wild-type and mutant enzymes
-
additional information
additional information
-
Km for fructose 1,6-diphosphate is dependent on the protein concentration in the range 0.01-0.05 mM
-
additional information
additional information
-
turnover-numbers of chimeric enzymes between two human aldolases A, B, or C
-