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Literature summary for 4.1.2.13 extracted from
- Expression, purification, crystallization and preliminary X-ray crystallographic analysis of fructose-1,6-bisphosphate aldolase from Escherichia coli (2014), Acta Crystallogr. F Struct. Biol. Commun., 70, 1376-1379 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystals of recombinant fructose-1,6-bisphosphate aldolase class I are obtained by the sitting-drop vapour-diffusion technique in a condition consisting of 19 mg/ml FBPA I in 0.1 M Tris pH 9.0, 10% (w/v) polyethylene glycol 8000 and diffract to 2.0 A resolution. The crystals belong to the monoclinic space group C2, with unit-cell parameters 217.7 A (a), 114.9 A (b), 183.9 A (c), 124.6° (beta) | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FBPA I | - |
Escherichia coli |
| fructose-1,6-bisphosphate aldolase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
12 h, enzyme retains almost 100% activity | Escherichia coli |
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