Cloned (Comment) | Organism |
---|---|
- |
Edwardsiella ictaluri |
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | isolation of thermostable variants of the class II fructose bisphosphate aldolase following four rounds of directed evolution using DNA shuffling of the fda gene. The variants show increased thermostability with no loss of catalytic function at room temperature | Edwardsiella ictaluri |
additional information | isolation of thermostable variants of the class II fructose bisphosphate aldolase following four rounds of directed evolution using DNA shuffling of the fda gene. The variants show increased thermostability with no loss of catalytic function at room temperature | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
D-fructose 1,6-bisphosphate | 30°C | Escherichia coli | |
0.15 | - |
D-fructose 1,6-bisphosphate | 30°C | Edwardsiella ictaluri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39026 | - |
2 * 39026, electrospray ionization mass spectrometry | Edwardsiella ictaluri |
77760 | - |
analytical ultracentrifugation | Edwardsiella ictaluri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Edwardsiella ictaluri | - |
- |
- |
Escherichia coli | P0AB71 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Edwardsiella ictaluri | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate | - |
Escherichia coli | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39026, electrospray ionization mass spectrometry | Edwardsiella ictaluri |
Synonyms | Comment | Organism |
---|---|---|
FBP-aldolase | - |
Edwardsiella ictaluri |
FBP-aldolase | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
50% loss of activity after incubation for 10 min at 49.5°C | Edwardsiella ictaluri |
51 | - |
50% loss of activity after incubation for 10 min at 50.5°C | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
D-fructose 1,6-bisphosphate | 30°C | Edwardsiella ictaluri | |
14.2 | - |
D-fructose 1,6-bisphosphate | 30°C | Escherichia coli |