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Literature summary for 4.1.2.13 extracted from

  • Hao, J.; Berry, A.
    A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents (2004), Protein Eng. Des. Sel., 17, 689-697.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Edwardsiella ictaluri
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information isolation of thermostable variants of the class II fructose bisphosphate aldolase following four rounds of directed evolution using DNA shuffling of the fda gene. The variants show increased thermostability with no loss of catalytic function at room temperature Edwardsiella ictaluri
additional information isolation of thermostable variants of the class II fructose bisphosphate aldolase following four rounds of directed evolution using DNA shuffling of the fda gene. The variants show increased thermostability with no loss of catalytic function at room temperature Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
D-fructose 1,6-bisphosphate 30°C Escherichia coli
0.15
-
D-fructose 1,6-bisphosphate 30°C Edwardsiella ictaluri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39026
-
2 * 39026, electrospray ionization mass spectrometry Edwardsiella ictaluri
77760
-
analytical ultracentrifugation Edwardsiella ictaluri

Organism

Organism UniProt Comment Textmining
Edwardsiella ictaluri
-
-
-
Escherichia coli P0AB71
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Edwardsiella ictaluri glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?
D-fructose 1,6-bisphosphate
-
Escherichia coli glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 39026, electrospray ionization mass spectrometry Edwardsiella ictaluri

Synonyms

Synonyms Comment Organism
FBP-aldolase
-
Edwardsiella ictaluri
FBP-aldolase
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
50% loss of activity after incubation for 10 min at 49.5°C Edwardsiella ictaluri
51
-
50% loss of activity after incubation for 10 min at 50.5°C Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13
-
D-fructose 1,6-bisphosphate 30°C Edwardsiella ictaluri
14.2
-
D-fructose 1,6-bisphosphate 30°C Escherichia coli