N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

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Word Map on EC


glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose
glycoprotein phospho-D-mannose


alpha-N-acetylglucosaminylphosphodiesterase, phosphodiester glycosidase, lysosomal alpha-N-acetyl-glucosaminidase, alpha-N-acetyl-D-glucosamine-1-phosphodiester acetamidodeoxyglucohydrolase, phosphodiester alpha-GlcNAcase, N-acetylglucosamine-1-phosphodiester N-acetylglucosaminidase, alpha-N-acetylglucosaminyl phosphodiesterase, lysosomal alpha-N-acetylglucosaminidase, alpha-N-acetyl-D-glucosamine-1-phosphodiester N-acetylglucosaminidase, 2-acetamido-2-deoxy-alpha-D-glucose 1-phosphodiester acetamidodeoxyglucohydrolase, UCE, mannose 6-phosphate-uncovering enzyme, NAGLU, mannose 6-phosphate uncovering enzyme, GH89, GlcNAc-1-phosphodiester-N-acetylglucosaminidase, uncovering enzyme, BACOVA_00430, GlcNAc-1-phosphodiester alpha-N-acetylglucosaminidase


     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
       N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase


Cloned on EC - N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

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CLONED (Commentary)
expressed as GFP-fusion protein in HeLa cells
expressed in Escherichia coli BL21 Star (DE3) cells
expression in COS-cells
expression in mouse L-cells, expression of UCE containing green fluorescent protein in place of the luminal domain in HeLa cells
expression in mouse L-cells, in CHO-K1 cells and in Sf9 insect cells
expression of the soluble form in COS cells
polymerase incomplete primer extension cloning method
polymerase incomplete primer extension cloning method, recombinant expression of enzyme with TEV cleavage site and His6-tag in Escherichia coli as wild-type and selenomethionine-labeled enzyme
two splice forms, one of these isoforms lacks 102-base pairs corresponding to exon 8 of the 10 exons present in the genomic DNA and is predominantly expressed in brain. Expression of wild-type and mutant enzymes in HeLa cells
wild-type and mutant UCE expression in mouse L-cells, expression of various green fluorescent protein-UCE fusion proteins in HeLa cells