3.1.4.45: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

This is an abbreviated version!
For detailed information about N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase, go to the full flat file.

Word Map on EC 3.1.4.45

Reaction

glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose
+
H2O
=
N-acetyl-D-glucosamine
+
glycoprotein phospho-D-mannose

Synonyms

alpha-N-acetylglucosaminylphosphodiesterase, phosphodiester glycosidase, lysosomal alpha-N-acetyl-glucosaminidase, alpha-N-acetyl-D-glucosamine-1-phosphodiester acetamidodeoxyglucohydrolase, phosphodiester alpha-GlcNAcase, N-acetylglucosamine-1-phosphodiester N-acetylglucosaminidase, alpha-N-acetylglucosaminyl phosphodiesterase, lysosomal alpha-N-acetylglucosaminidase, alpha-N-acetyl-D-glucosamine-1-phosphodiester N-acetylglucosaminidase, 2-acetamido-2-deoxy-alpha-D-glucose 1-phosphodiester acetamidodeoxyglucohydrolase, UCE, mannose 6-phosphate-uncovering enzyme, NAGLU, mannose 6-phosphate uncovering enzyme, GH89, GlcNAc-1-phosphodiester-N-acetylglucosaminidase, uncovering enzyme, BACOVA_00430, GlcNAc-1-phosphodiester alpha-N-acetylglucosaminidase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.45 N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Cloned

Cloned on EC 3.1.4.45 - N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as GFP-fusion protein in HeLa cells
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expressed in Escherichia coli BL21 Star (DE3) cells
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expression in COS-cells
expression in mouse L-cells, expression of UCE containing green fluorescent protein in place of the luminal domain in HeLa cells
-
expression in mouse L-cells, in CHO-K1 cells and in Sf9 insect cells
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expression of the soluble form in COS cells
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polymerase incomplete primer extension cloning method
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polymerase incomplete primer extension cloning method, recombinant expression of enzyme with TEV cleavage site and His6-tag in Escherichia coli as wild-type and selenomethionine-labeled enzyme
two splice forms, one of these isoforms lacks 102-base pairs corresponding to exon 8 of the 10 exons present in the genomic DNA and is predominantly expressed in brain. Expression of wild-type and mutant enzymes in HeLa cells
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wild-type and mutant UCE expression in mouse L-cells, expression of various green fluorescent protein-UCE fusion proteins in HeLa cells
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