N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

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Word Map on EC


glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose
glycoprotein phospho-D-mannose


alpha-N-acetylglucosaminylphosphodiesterase, phosphodiester glycosidase, lysosomal alpha-N-acetyl-glucosaminidase, alpha-N-acetyl-D-glucosamine-1-phosphodiester acetamidodeoxyglucohydrolase, phosphodiester alpha-GlcNAcase, N-acetylglucosamine-1-phosphodiester N-acetylglucosaminidase, alpha-N-acetylglucosaminyl phosphodiesterase, lysosomal alpha-N-acetylglucosaminidase, alpha-N-acetyl-D-glucosamine-1-phosphodiester N-acetylglucosaminidase, 2-acetamido-2-deoxy-alpha-D-glucose 1-phosphodiester acetamidodeoxyglucohydrolase, UCE, mannose 6-phosphate-uncovering enzyme, NAGLU, mannose 6-phosphate uncovering enzyme, GH89, GlcNAc-1-phosphodiester-N-acetylglucosaminidase, uncovering enzyme, BACOVA_00430, GlcNAc-1-phosphodiester alpha-N-acetylglucosaminidase


     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
       N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase


Crystallization on EC - N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

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purified recombinant detagged wild-type and selenomethionine-labeled enzyme, sitting nanodroplet vapor diffusion method, mixing of 200 nl of 21 mg/ml protein in 20 mM HEPES, pH 8.0, 200 mM NaCl, 40 mM imidazole, and 1 mM TCEP, with 200 nl of reservoir solution containing 0.2 M Li2SO4, 30% PEG 4000, and 0.1 M Tris, pH 8.5, and equilibration against 0.05 ml of reservoir solution, 4°C, 10% v/v ethylene glycol as a cryoprotectant, X-ray diffraction structure determination and analysis at 1.80 A resolution, modeling, the crystal structure of the BACOVA_00430 protein from Bacteroides ovatus is used to model the luminal portion of the human enzyme N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
hanging drop vapour diffusion method, in 2.0 M (NH4)2SO4 with 3% (v/v) glycerol