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Literature summary for 3.1.4.45 extracted from

  • Rohrer, J.; Kornfeld, R.
    Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network (2001), Mol. Biol. Cell, 12, 1623-1631.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.45

Cloned(Commentary)

Cloned (Comment) Organism
expression in mouse L-cells, expression of UCE containing green fluorescent protein in place of the luminal domain in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H510stop mutant of the cytosolic tail disrupting the NPF endocytosis motif Homo sapiens
Y488A mutant of the cytosolic tail disrupting the tyrosine-based endocytosis motif Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane resides in the trans-Golgi network and cycles between the network and plasma membrane, type I membrane-spanning glycoprotein with a single 27-residue transmembrane domain and a 41-residue cytoplasmic tail Homo sapiens 139
-
plasma membrane resides in the trans-Golgi network and cycles between the network and plasma membrane Homo sapiens 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 x * 80000, sialylated UCE, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O Homo sapiens catalyzes a crucial step in lysosomal biogenesis, removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal hydrolases N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein UCE is core glycosylated as it enters the endoplasmic reticulum, traverses the Golgi apparatus where the oligosaccahide is processed to a complex-type structure Homo sapiens
sialoprotein addition of sialic acid in the trans-Golgi network Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal hydrolases Homo sapiens N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose
-
 ?
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O catalyzes a crucial step in lysosomal biogenesis, removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal hydrolases Homo sapiens N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, sialylated UCE, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
2-acetamido-2-deoxy-alpha-D-glucose 1-phosphodiester acetamidodeoxyglucohydrolase
-
 Homo sapiens
alpha-N-acetyl-D-glucosamine-1-phosphodiester N-acetylglucosaminidase
-
 Homo sapiens
alpha-N-acetylglucosaminyl phosphodiesterase
-
 Homo sapiens
lysosomal alpha-N-acetylglucosaminidase
-
 Homo sapiens
UCE uncovering enzyme Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens