Literature summary for 3.1.4.45 extracted from

Wei, Y.; Yen, T.Y.; Cai, J.; Trent, J.O.; Pierce, W.M.; Young, W.W., Jr.
Structural features of the lysosomal hydrolase mannose 6-phosphate uncovering enzyme (2005), Glycoconj. J., 22, 13-19.

Search for more literature for 3.1.4.45

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the soluble form in COS cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	soluble form lacking residues 1-49, transmembrane and cytoplasmic domains are replaced by the HPC4 epitope	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	1885 Da, peptides of amino acid position 80 to 84 and 268 to 274, determined by LC/ESI-MS/MS analysis, soluble form	Homo sapiens
additional information	-	2797 Da, peptide of amino acid position 159 to 183, determined by MALDI-TOF and by LC/MS, soluble form	Homo sapiens
additional information	-	2878 Da, peptide of amino acid position 331 to 355, determined by LC/MS, soluble form	Homo sapiens
additional information	-	2925 Da, peptide of amino acid position 305 to 355, determined by MALDI-TOF and by LC/MS, soluble form	Homo sapiens
additional information	-	3124 Da, peptides of amino acid position 54 to 69 and 94 to 108, determined by LC/ESI-MS/MS analysis, soluble form	Homo sapiens
additional information	-	3230 Da, peptides of amino acid position 1 to 4 and 159 to 182, determined by LC/ESI-MS/MS analysis, soluble form	Homo sapiens
additional information	-	4023 Da, peptide of amino acid position 228 to 264, determined by MALDI-TOF and by LC/MS, soluble form	Homo sapiens
additional information	-	4337 Da, peptides of amino acid position 228 to 264 and 265 to 267, determined by LC/ESI-MS/MS analysis, soluble form	Homo sapiens
additional information	-	880 Da, peptide of amino acid position 369 to 376, determined by MALDI-TOF, soluble form	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	soluble form where transmembrane and cytoplasmic domains were replaced with the HPC4 epitope	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	N-linked glycosylation of Asn159, Asn165, Asn247 and Asn317 determined by PNGase F treatment and MS analysis, soluble form	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
affinity chromatography on HPC4 monoclonal antibody column	Homo sapiens

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Release 2023.1 (January 2023)