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Literature summary for 3.1.4.45 extracted from

  • Kornfeld, R.; Bao, M.; Brewer, K.; Noll, C.; Canfield, W.
    Molecular cloning and functional expression of two splice forms of human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (1999), J. Biol. Chem., 274, 32778-32785.
    View publication on PubMed
Search for more literature for 3.1.4.45

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose full length enzyme Homo sapiens
0.43
-
 methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose delta 102-bp variant Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 brain, reducing SDS-PAGE Homo sapiens
77000
-
 liver, reducing SDS-PAGE Homo sapiens
118000
-
 serum Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UK23
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glycoprotein, N-glycosylated, contains sialic acid Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O
-
 Homo sapiens N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 69000, gel filtration and reducing SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens