3.1.3.48: protein-tyrosine-phosphatase
This is an abbreviated version!
For detailed information about protein-tyrosine-phosphatase, go to the full flat file.
Word Map on EC 3.1.3.48
-
3.1.3.48
-
pp2a
-
mapks
-
autoimmune
-
erk
-
cyclin
-
leukemia
-
endothelial
-
ras
-
signal-regulated
-
lysosomal
-
okadaic
-
t-cells
-
metastasis
-
checkpoint
-
sirna
-
obesity
-
myeloid
-
3-kinase
-
adaptor
-
arthritis
-
vanadate
-
stat3
-
tumorigenesis
-
prostate
-
mellitus
-
phosphatidylinositol
-
cyclin-dependent
-
c-jun
-
pkc
-
rheumatoid
-
tyrosine-phosphorylated
-
hyperphosphorylation
-
nonreceptor
-
chk1
-
cytochemical
-
autophosphorylation
-
receptor-like
-
genistein
-
irs-1
-
insulin-stimulated
-
phosphopeptide
-
tartrate-resistant
-
grb2
-
c-src
-
jak2
-
fyn
-
paxillin
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substrate-1
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myelomonocytic
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polo-like
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medicine
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pharmacology
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diagnostics
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drug development
- 3.1.3.48
- pp2a
- mapks
- autoimmune
- erk
- cyclin
- leukemia
- endothelial
- ras
-
signal-regulated
- lysosomal
-
okadaic
- t-cells
- metastasis
-
checkpoint
- sirna
- obesity
- myeloid
- 3-kinase
- adaptor
- arthritis
- vanadate
- stat3
- tumorigenesis
- prostate
- mellitus
- phosphatidylinositol
-
cyclin-dependent
- c-jun
- pkc
-
rheumatoid
-
tyrosine-phosphorylated
-
hyperphosphorylation
-
nonreceptor
- chk1
-
cytochemical
- autophosphorylation
-
receptor-like
- genistein
- irs-1
-
insulin-stimulated
- phosphopeptide
-
tartrate-resistant
- grb2
- c-src
- jak2
- fyn
- paxillin
- substrate-1
-
myelomonocytic
-
polo-like
- medicine
- pharmacology
- diagnostics
- drug development
Reaction
Synonyms
70Z-SHP, acid phosphatase, acid phosphatase activity, acid phosphatase ortholog, acid tyrosine phosphatase, alpha-PTP-PEST, AUM, Bd1204, BDP, Bmptp-h, Brain-derived phosphatase, BVP, CAPTPase, CD148, CD148 antigen, CD45, CD45 antigen, CD45 protein tyrosine phosphatase, Cdc25 phosphatase, Cdc25-like protein, Cdc25A, Cdc25B, Cdc25B phosphatase, Cdc25C, CDK2-associated dual specificity phosphatase, Ch-1PTPase, chick retinal tyrosine phosphatase-2, cold-active protein tyrosine phosphatase, CPTP1, CQMa102 phosphatase, Cryp-2, CRYP-2/PTPRO, CRYP-2/receptor-type tyrosine-protein phosphatase O, cysteine-dependent protein tyrosine phosphatase, cyt-PTPepsilon, density-enhanced phosphatase-1, DEP-1, DEP-1 phosphatase, DLAR, dPTP61F, DSP, DSP18, dual specificity phosphatase, Dual specificity phosphatase Cdc25A, Dual specificity phosphatase Cdc25B, Dual specificity phosphatase Cdc25C, dual specificity protein phosphatase, dual specificity protein phosphatase 23, dual specificity protein phosphatase 3, Dual specificity protein phosphatase hVH1, Dual specificity protein phosphatase hVH2, Dual specificity protein phosphatase hVH3, Dual specificity protein phosphatase PYST1, Dual specificity protein phosphatase PYST2, Dual specificity protein phosphatase VHR, dual-specificity (Thr/Tyr) MAPK protein phosphatase, dual-specificity phosphatase, dual-specificity protein phosphatase, dual-specificity protein tyrosine phosphatase 18, Dual-specificity tyrosine phosphatase TS-DSP6, Dual-specificity tyrosine phosphatase YVH1, DUSP, ED-Eya2, EhPRL, EpsB, ES cell phosphatase, FAP-1, Fap1, FLP1, GLEPP-1, GLEPP1, HAP, HCP, HD-PTP, Hematopoietic cell protein-tyrosine phosphatase, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, hematopoietic protein tyrosine phosphatase, Hematopoietic protein-tyrosine phosphatase, hematopoietic tyrosine phosphatase, HEPTP, His domain protein tyrosine phosphatase, histidine acid phosphatase, histidine domain-protein tyrosine phosphatase, HPTP beta-like tyrosine phosphatase, HPTP eta, HPTPbeta, HPTPbeta-CD, hPTPE1, IA-2, IA-2A, IA-2beta, IAR, ICAAR, IF1, IF2, insulinoma associated protein tyrosine phosphatase 2, islet cell antigen-related PTP, Islet cell autoantigen related protein, KIM-containing PTP, KIM-PTP, kinase associated phosphatase, kinase interaction motif phosphatase, kinase interaction motif-containing protein tyrosine phosphatases, L-CA, LAR, Late protein H1, LCA, LCA-related phosphatase, LDP-3, leucocyte common antigen-related protein tyrosine phosphatase, Leukocyte antigen related, leukocyte common antigen-related, LmACR2, LMJF_16_0230, LmPRL-1, LMW PTPase, LMW-PTP, LMWPTP, low molecular mass dual specificity phosphatase 3, Low molecular weight cytosolic acid phosphatase, Low molecular weight phosphotyrosine protein phosphatase, low molecular weight protein tyrosine phosphatase, low molecular weight protein-tyrosine phosphatase A, low molecular weight protein-tyrosine-phosphatase, low molecular weight-PTP, low-molecular weight PTPase, low-molecular-weight protein tyrosine phosphatase, Lpt1, LRP, Lymphoid phosphatase, lymphoid-specific tyrosine phosphatase, LyP, M1851, MAP kinase phosphatase, MAP-kinase phosphatase CPG21, MAPK-specific tyrosine phosphatase, MEG, mitogen-activated protein kinase phosphatase, mitogen-activated protein kinase phosphatase-1, mitogen-activated protein kinase-specific tyrosine phosphatase, Mitosis initiation protein, Mitosis initiation protein MIH1, Mitotic inducer homolog, MKP, MKP-1, MKP-1 like protein tyrosine phosphatase, MKP-2, MKP-3, MKP-4, MKP-5, MKP-7, MKP-X, MKP1, MKP5-C, More, MPTP, MPTP-PEST, MPtpA, MPtpB, MSP, N3PTP, NC-PTPCOM1, Neural-specific protein-tyrosine phosphatase, non-receptor protein tyrosine phosphatase, ORF5, orphan protein tyrosine phosphatase, OST-PTP, osteoclastic protein-tyrosine phosphatase, P19-PTP, p52shc, p65 PTPepsilon, p66shc, p67 PTPepsilon, P80, PC12-PTP1, PCPTP1, PEST, PGN_1525, Pgp, Phogrin, Phosphacan, phosphatase of regenerating liver-3, phosphatase, phosphoprotein (phosphotyrosine), phosphatase, phosphotyrosine, phosphoprotein phosphatase (phosphotyrosine), phosphotyrosine histone phosphatase, phosphotyrosine phosphatase, Phosphotyrosine phosphatase 13, phosphotyrosine phosphatase 1B, phosphotyrosine protein phosphatase, phosphotyrosine-specific PP, phosphotyrosylprotein phosphatase, Pmp1p, PP2A, PPase, pphA, PPM, PPT-phosphatase, PRL, PRL-1, PRL-3, PRL-related protein tyrosine phosphatase, probable low molecular weight protein-tyrosine-phosphatase, protein of regenerating liver-related protein tyrosine phosphatase, Protein phosphatase 1B, protein phosphatase 2A, protein phosphotyrosine phosphatase, protein Tyr phosphatase, protein tyrosine phosphatase, protein tyrosine phosphatase 1B, protein tyrosine phosphatase A, protein tyrosine phosphatase alpha, protein tyrosine phosphatase B, protein tyrosine phosphatase beta, protein tyrosine phosphatase epsilon, protein tyrosine phosphatase eta, protein tyrosine phosphatase H1, protein tyrosine phosphatase N12, protein tyrosine phosphatase N3, protein tyrosine phosphatase ny, Protein tyrosine phosphatase receptor-type T, protein tyrosine phosphatase SHP-1, protein tyrosine phosphatase sigma, protein tyrosine phosphatase xi, protein tyrosine phosphatase, non-receptor type 1, protein tyrosine phosphatase, receptor-type, Z polypeptide 1, protein tyrosine phosphatase-1B, protein tyrosine phosphatase-2, protein tyrosine phosphatase-BL, protein tyrosine phosphatase-H2, Protein tyrosine phosphatase-NP, protein tyrosine phosphatase-PEST, Protein-protein-tyrosine phosphatase HA2, protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1B, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase 1E, Protein-tyrosine phosphatase 2C, Protein-tyrosine phosphatase 2E, Protein-tyrosine phosphatase 3CH134, protein-tyrosine phosphatase alpha, Protein-tyrosine phosphatase CL100, Protein-tyrosine phosphatase D1, Protein-tyrosine phosphatase ERP, Protein-tyrosine phosphatase G1, Protein-tyrosine phosphatase H1, Protein-tyrosine phosphatase LC-PTP, Protein-tyrosine phosphatase MEG1, Protein-tyrosine phosphatase MEG2, Protein-tyrosine phosphatase P19, Protein-tyrosine phosphatase PCPTP1, Protein-tyrosine phosphatase pez, Protein-tyrosine phosphatase PTP-RL10, Protein-tyrosine phosphatase PTP36, Protein-tyrosine phosphatase PTPL1, Protein-tyrosine phosphatase striatum-enriched, Protein-tyrosine phosphatase SYP, Protein-tyrosine-phosphatase SL, Protein-tyrosine-phosphate phosphohydrolase, Prp1, PrpA, PTEN, PTEN phosphoprotein phosphatase, PTK, PTP, PTP 1B, PTP epsilon, PTP IA-2beta, PTP-1B, PTP-1C, PTP-1D, PTP-2C, PTP-BAS, PTP-BL, PTP-E1, PTP-eta, PTP-F, PTP-H1, PTP-H2, PTP-HA2, PTP-like phytase, PTP-MEG2, PTP-NP, PTP-oc, PTP-PEST, PTP-phosphatase, PTP-SH2beta, PTP-SL, PTP-U2, PTP1, PTP1B, PTP1C, PTP1D, PTP1e, PTP1N13, PTP1N21, PTP2C, PTP3, PTP36, PtpA, PTPalpha, PTPase, PTPase YVH1, PTPase-MEG1, PTPase-MEG2, PtpB, PTPB1, PTPBAS, PTPbeta, PTPBR7, PTPD1, PTPepsilon, PTPepsilonC, PTPepsilonM, PTPeta, PTPetaCD, PTPG1, PTPgamma, PTPH1, PTPL1, PTPLP, PTPMEG, PTPN1, PTPN11, PTPN12, PTPN13, PTPN14, PTPN2, PTPN20 variant 15, PTPN21, PTPN22, PTPN23, PTPN3, PTPN4, PTPN4/PTP-MEG1, PTPN5, PTPN6, PTPN7, PTPN9, PTPNE6, PTPP, PTPPase, PTPPBSgamma, PTPPBSgamma-37, PTPPBSgamma-42, PTPRA, PTPRB, PTPRC, PTPRD, PTPRdelta, PTPRE, PTPRF, PTPRG, PTPRH, PTPRJ, PTPRK, PTPRM, PTPRN, PTPRN2, PTPRO, PTPRO, truncated, PTPRO-FL, PTPRomega, PTPROt, PTPRQ, PTPRR, PTPRS, PTPRT, PTPRU, PTPRV, PTPRZ, PTPRZ1, PTPS31, PTPsigma, PTPxi/RPTPbeta, PY protein phosphatase, Pyp1p, Pyp2p, R-PTP-alpha, R-PTP-beta, R-PTP-delta, R-PTP-epsilon, R-PTP-eta, R-PTP-gamma, R-PTP-kappa, R-PTP-mu, R-PTP-zeta, R2B phosphatase, receptor protein tyrosine phosphatase, receptor protein tyrosine phosphatase isoform delta, receptor protein tyrosine phosphatase isoform omega, receptor protein tyrosine phosphatase rho, receptor protein tyrosine phosphatase T, receptor protein tyrosine phosphatase {kappa}, receptor PTPepsilon, Receptor-linked protein-tyrosine phosphatase 10D, Receptor-linked protein-tyrosine phosphatase 99A, receptor-type protein tyrosine phosphatase J, Receptor-type protein-tyrosine phosphatase-kappa, receptor-type tyrosine-protein phosphatase R, reductant-inhibited PTPase1, RIP1, RNA/RNP complex-intereracting phosphatase, ROL B protein, RPTP, RPTP-BK, RPTPalpha, RPTPdelta, RPTPepsilon, RPTPgamma, RPTPkappa, RPTPlambda, RPTPmu, RPTPrho, RPTPrho/PTPRT, RPTPsigma, RPTPzeta, Saci-PTP, Saci0545, SAP1, Scr homology 2-containing tyrosine phosphatase, selective striatal enriched protein phosphatase, SH-PTP1, SH-PTP2, SH-PTP3, SH2 domain-containing tyrosine phosphatase-1, SH2 domain-containing tyrosine phosphatase-2, SHP, SHP-1, SHP-2, SHP1, SHP2, Siw14, Slr0328, Small tyrosine phosphatase, Small, acidic phosphotyrosine protein phosphatase, SP-PTP, Spd1837, SPD_1837, specific protein-tyrosine phosphatase, SPH-1, SPH-2, SPy_0036, SPy_0039, Src homology 2 domain-containing phosphatase-1, Src homology region 2 domain-containing phosphatase 1, Src homology-2 domain containing protein tyrosine phosphatase-1, Src homology-2 domain containing protein tyrosine phosphatase-2, STEP, STEP33, STEP46, STEP61, Stp1, striatal enriched protein tyrosine phosphatase, striatal-enriched protein-tyrosine phosphatase, striatal-enriched PTP, String protein, SynPTP, Syp, T cell protein tyrosine phosphatase, T-cell protein tyrosine phosphatase, T-cell protein-tyrosine phosphatase, T-cell PTP, T-DSP11, T200, TC PTP, TC-PTPase ´, TC48, TCPTP, TCPTP/TC45, TcPTP1, testis- and skeletal-muscle-specific DSP, testis- and skeletal-muscle-specific dual specificity protein phosphatase, Testis-and skeletal-muscle-specific DSP, Tk-PTP, TK0241, TMDP, TpbA, TypPT, tyrosine O-phosphate phosphatase, tyrosine phosphatase, tyrosine phosphatase alpha, Tyrosine phosphatase CBPTP, tyrosine phosphatase epsilon, tyrosine phosphatase IA-2, tyrosine phosphatase Src homology region 2 domain-containing phosphatase 1, tyrosine phosphatase-1B, tyrosine-protein phosphatase, tyrosine-protein phosphatase non-receptor type 22, tyrosine-protein phosphatase non-receptor type 5, tyrosine-specific MAPK phosphatase, tyrosylprotein phosphatase, vaccinia H1-related PTP, VE-PTP, VHR, VHZ, Wzb, YopH, YwlE, YwqE, [phosphotyrosine]protein phosphatase
ECTree
3.1.3.48 protein-tyrosine-phosphataseAdvanced search results
results (
results (KM Value
KM Value on EC 3.1.3.48 - protein-tyrosine-phosphatase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.16
3-O-methylfluorescein phosphate
-
in 50 mM sodium acetate (pH 5.5), 80 mM NaCl, 10 mM dithiothreitol, and 20% glycerol, at 22°C
2.6
AAAAApYRHRR
-
pH 7.4, temperature not specified in the publication, RPTPalpha
0.49
ARKRIpYAA
-
pH 7.4, temperature not specified in the publication, RPTPalpha
0.00044
casein
-
isoform SynPPM3, activity against phosphoserine residues, pH 7.5, 37°C
0.0005
Cdk2-pTpY/CycA
-
in 50 mM Tris, 50 mM Bis-Tris, 100 mM Na acetate, pH 6.0-6.5, at 5-25°C
-
0.574
Inositol hexakisphosphate
in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C
0.77
LIEDNEpYTARQGA
-
pH 7.4, temperature not specified in the publication, RPTPalpha
0.127
PGSAAP-pY-LKTKFI
pH 6.4, 25°C, recombinant PTPepsilon D1 domain, substrate is a STAT3 peptide
0.059 - 0.789
platelet-derived growth factor receptor
value varies with the point of tyrosine phosphorylation, values for truncated enzyme reduced relative to wild-type form
-
0.119
PQDKEY-pY-KVKEPG
pH 6.4, 25°C, recombinant PTPepsilon D1 domain, substrate is a JAK2 peptide
0.124
RKGSGD-pY-MPMSPK
pH 6.4, 25°C, recombinant PTPepsilon D1 domain, substrate is a IRS1 peptide
1.07
TATEPQpYQPGEN
-
pH 7.4, temperature not specified in the publication, RPTPalpha
0.0215
[Eps15 peptide846-854 P850V]-tyrosine phosphate
recombinant wild-type enzyme, pH and temperature not specified in the publication
-
0.0438
[Eps15 peptide846-854]-tyrosine phosphate
recombinant wild-type enzyme, pH and temperature not specified in the publication
-
0.02
4,6,8-trimethyl-2-oxo-2H-chromen-7-yl dihydrogen phosphate
-
isoform PTP1B, 37°C, pH 6.9
0.115
4,6,8-trimethyl-2-oxo-2H-chromen-7-yl dihydrogen phosphate
-
37°C, pH 6.9
0.156
4,6,8-trimethyl-2-oxo-2H-chromen-7-yl dihydrogen phosphate
-
isoform CD45, 37°C, pH 6.9
0.00932
4-nitrophenyl phosphate
pH 6.0, 37°C, recombinant His-tagged enzyme
0.45
4-nitrophenyl phosphate
mutant enzyme I115F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.46
4-nitrophenyl phosphate
mutant enzyme I115L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.46
4-nitrophenyl phosphate
mutant enzyme I115M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.48
4-nitrophenyl phosphate
mutant enzyme V79I, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.51
4-nitrophenyl phosphate
mutant enzyme L145A, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.55
4-nitrophenyl phosphate
mutant enzyme V85F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.56
4-nitrophenyl phosphate
mutant enzyme D236A/Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0
0.59
4-nitrophenyl phosphate
mutant enzyme L145V, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.6
4-nitrophenyl phosphate
mutant enzyme I115V, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.6
4-nitrophenyl phosphate
mutant enzyme V85M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.62
4-nitrophenyl phosphate
wild type enzyme, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.66
4-nitrophenyl phosphate
mutant enzyme V79L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.71
4-nitrophenyl phosphate
mutant enzyme L145F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.8
4-nitrophenyl phosphate
mutant enzyme V85L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.83
4-nitrophenyl phosphate
mutant enzyme L145M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
0.89 - 57.7
4-nitrophenyl phosphate
-
value strongly dependent on ionic strength and pH
1.31
4-nitrophenyl phosphate
mutant enzyme D236A, at 30°C, in 0.15 M Bis-Tris, pH 6.0
2.11
4-nitrophenyl phosphate
mutant enzyme Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0
2.4
4-nitrophenyl phosphate
mutant enzyme I307L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
3.26
4-nitrophenyl phosphate
wild type enzyme, at 30°C, in 0.15 M Bis-Tris, pH 6.0
4.01
4-nitrophenyl phosphate
mutant enzyme I307A, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
5.03
4-nitrophenyl phosphate
mutant enzyme V79A, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
6
4-nitrophenyl phosphate
mutant enzyme I307F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
6.3
4-nitrophenyl phosphate
mutant enzyme I307M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C
8
4-nitrophenyl phosphate
recombinant enzyme, pH 7.0, 37°C
0.0111
6,8-difluoro-4-methylumbelliferyl phosphate
MKP-4, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol
0.0173
6,8-difluoro-4-methylumbelliferyl phosphate
MKP-3, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol
0.023
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 60°C, mutant E132A
0.0274
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 60°C, mutant D63A
0.0448
6,8-difluoro-4-methylumbelliferyl phosphate
VHR, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol
0.0658
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 20°C, mutant E132A
0.0666
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 20°C, mutant D63A
0.0682
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 20°C, mutant G95A
0.0795
6,8-difluoro-4-methylumbelliferyl phosphate
MKP-5, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol
0.106
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 60°C, mutant G95A
0.135
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 60°C, mutant Q136A
0.15
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 60°C, mutant E132Q
0.223
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 60°C, mutant D63N/E132A
0.251
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 60°C, mutant E132L
0.252
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 60°C, mutant D63N
0.258
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 60°C, mutant R124E
0.276
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 60°C, wild-type enzyme
0.301
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 60°C, mutant R124A
0.307
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 20°C, mutant E132Q
0.363
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 20°C, mutant Q136A
0.363
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 20°C, mutant R124E
0.466
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 20°C, mutant D63N/E132A
0.56
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 20°C, mutant D63N
0.611
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 20°C, mutant R124A
0.719
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 20°C, wild-type enzyme
0.862
6,8-difluoro-4-methylumbelliferyl phosphate
pH 5.0, 20°C, mutant E132L
0.00011
bovine serum albumin
-
calculated as mM phosphate released, enzyme subtype 1B, cytoplasmic and membrane-bound form
-
0.00026
bovine serum albumin
-
calculated as mM phosphate released, enzyme subtype 1A, cytoplasmic form
-
0.00045
carboxyamidomethylated and maleylated lysozyme
-
isoform SynPPM3, activity against phosphotyrosine residues, pH 7.5, 37°C
-
0.0017
carboxyamidomethylated and maleylated lysozyme
-
isoform SynPPP1, activity against phosphoserine residues, pH 7.5, 37°C
-
0.0027
DADEpYLIPQQG
wild type PTPH1, in the presence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C
0.0031
DADEpYLIPQQG
wild type PTPH1, in the absence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C
0.0035
DADEpYLIPQQG
CCPGCC-insertion mutant PTPH1-816, in the absence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C
0.0077
DADEpYLIPQQG
CCPGCC-insertion mutant PTPH1-816, in the presence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C
0.077
DADEpYLIPQQG
wild type PTPalpha, in the presence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C
0.082
DADEpYLIPQQG
wild type PTPalpha, in the absence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C
0.084
DADEpYLIPQQG
CCPGCC-insertion mutant PTPalpha-406, in the absence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C
0.1
DADEpYLIPQQG
CCPGCC-insertion mutant PTPalpha-406, in the presence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C
0.0096
GNGDpYMPMSPKS
-
reaction catalyzed by the full-length enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C
0.0191
GNGDpYMPMSPKS
-
reaction catalyzed by the catalyctic domain of the enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C
0.00028
lysozyme
-
calculated as mM phosphate released, enzyme subtype 1B, cytoplasmic and membrane-bound form
-
0.00045
lysozyme
-
calculated as mM phosphate released , enzyme subtype 1A, cytoplasmic form
-
0.00014
myelin basic protein
-
calculated as mM phosphate released, enzyme subtype 1A, cytoplasmic form
-
0.00025
myelin basic protein
-
calculated as mM phosphate released, enzyme subtype 1B, cytoplasmic and membrane-bound form
-
0.0063
myelin basic protein
-
isoform SynPPM3, activity against phosphoserine residues, pH 7.5, 37°C
-
0.022
myelin basic protein
-
isoform SynPPP1, activity against phosphoserine residues, pH 7.5, 37°C
-
1.7
p-nitrophenyl phosphate
-
reaction catalyzed by the full-length enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C
2.7
p-nitrophenyl phosphate
-
reaction catalyzed by the catalyctic domain of the enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C
10
p-nitrophenyl phosphate
-
in 50 mM Tris, 50 mM Bis-Tris, 100 mM Na acetate, pH 6.0-6.5, at 5-25°C
0.53
SASASpYSASA
-
pH 7.4, temperature not specified in the publication, RPTPalpha
1.16
TSTEPQpYQPGENL
-
pH 7.4, temperature not specified in the publication, RPTPalpha
0.0032
WDEDFpYDWEF
-
pH 7.4, temperature not specified in the publication, SHP-1
0.0063
WDEDFpYDWEF
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pH 7.4, temperature not specified in the publication, SHP-2
additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
additional information
Michaelis-Menten kinetics
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kinetics of WPD loop insertion cysteine mutants, overview
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additional information
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kinetic analyses of the effects of D92A and G129E mutations on the phospholipid and phosphoprotein phosphatase activities of PTEN
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additional information
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Michaelis-Menten steady state kinetics with PTP pseudo-substrate 4-nitrophenyl phosphate
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additional information
Michaelis-Menten and Lineweaver-Burk kinetics
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additional information
additional information
Michaelis-Menten and Lineweaver-Burk kinetics
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additional information
additional information
kinetic isotope effects. In the Stp reaction protonation of the leaving group lags behind P-O bond cleavage, evidenced by the small normal 15(V/K) values indicating a partial negative charge on the leaving group, as well as the more normal 18(V/K)bridge KIEs. The active form of the substrate is the dianion
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additional information
additional information
kinetic isotope effects. In the VHZ reaction protonation of the leaving group lags behind P-O bond cleavage, evidenced by the small normal 15(V/K) values indicating a partial negative charge on the leaving group, as well as the more normal 18(V/K)bridge KIEs. The active form of the substrate is the dianion
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additional information
additional information
kinetic isotope effects. In the VHZ reaction protonation of the leaving group lags behind P-O bond cleavage, evidenced by the small normal 15(V/K) values indicating a partial negative charge on the leaving group, as well as the more normal 18(V/K)bridge KIEs. The active form of the substrate is the dianion
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additional information
additional information
kinetic isotope effects. In the VHZ reaction protonation of the leaving group lags behind P-O bond cleavage, evidenced by the small normal 15(V/K) values indicating a partial negative charge on the leaving group, as well as the more normal 18(V/K)bridge KIEs. The active form of the substrate is the dianion
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additional information
additional information
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kinetic isotope effects. The active form of the substrate is the dianion
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additional information
additional information
kinetic isotope effects. The active form of the substrate is the dianion
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additional information
additional information
kinetic isotope effects. The active form of the substrate is the dianion
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additional information
additional information
kinetic isotope effects. The active form of the substrate is the dianion
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additional information
additional information
kinetic isotope effects. The active form of the substrate is the dianion
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additional information
additional information
Michaelis-Menten kinetic modeling
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additional information
additional information
steady-state kinetic study, Lineweaver-Burk plots
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