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Literature summary for 3.1.3.48 extracted from

  • Li, R.; Potters, M.B.; Shi, L.; Kennelly, P.J.
    The protein phosphatases of Synechocystis sp. strain PCC 6803: open reading frames sll1033 and sll1387 encode enzymes that exhibit both protein-serine and protein-tyrosine phosphatase activity in vitro (2005), J. Bacteriol., 187, 5877-5884.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.48

Protein Variants

Protein Variants Comment Organism
D415A isoform SynPPM3, no activity with 4-nitrophenyl phosphate, reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
D415N isoform SynPPM3, 5% activity with 4-nitrophenyl phosphate compared to wild-type, 50% reduction in phosphotyrosine-activity against carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
D444N isoform SynPPM3, no activity with 4-nitrophenyl phosphate, reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
D604N isoform SynPPM3, no activity Synechocystis sp.
D608N isoform SynPPM3, 50% activity with 4-nitrophenyl phosphate compared to wild-type, 4fold increase in phosphotyrosine-activity against carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
D648N isoform SynPPM3, 5% activity with 4-nitrophenyl phosphate compared to wild-type, large reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
E414Q isoform SynPPM3, similar activity with 4-nitrophenyl phosphate as wild-type, but 50% reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
E618Q isoform SynPPM3, 87% of activity with 4-nitrophenyl phosphate compared to wild-type, reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
NaF moderate inhibition, both SynPPP1 and SYnPPM3 Synechocystis sp.
o-vanadate both SynPPP1 and SYnPPM3 Synechocystis sp.
okadaic acid both SynPPP1 and SYnPPM3 Synechocystis sp.
Sodium diphosphate both isoforms, inhibition by millimolar concentrations Synechocystis sp.
Tartrate both SynPPP1 and SYnPPM3 Synechocystis sp.
tetramisole both SynPPP1 and SYnPPM3 Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00044
-
 casein isoform SynPPM3, activity against phosphoserine residues, pH 7.5, 37°C Synechocystis sp.
0.00045
-
 carboxyamidomethylated and maleylated lysozyme isoform SynPPM3, activity against phosphotyrosine residues, pH 7.5, 37°C Synechocystis sp.
0.0017
-
 carboxyamidomethylated and maleylated lysozyme isoform SynPPP1, activity against phosphoserine residues, pH 7.5, 37°C Synechocystis sp.
0.0063
-
 myelin basic protein isoform SynPPM3, activity against phosphoserine residues, pH 7.5, 37°C Synechocystis sp.
0.022
-
 myelin basic protein isoform SynPPP1, activity against phosphoserine residues, pH 7.5, 37°C Synechocystis sp.
12.7
-
 4-nitrophenyl phosphate isoform SynPPM3, pH 7.5, 37°C Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 strain PCC 6803, isoforms SynPPP1 and SynPPM3 both acting on phosphotyrosine- as well as phosphoserine-containing proteins
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.066
-
 isoform SynPPM3, pH 7.5, 37°C Synechocystis sp.
0.084
-
 isoform SynPPP1, pH 7.5, 37°C Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Synechocystis sp. 4-nitrophenol + phosphate
-
 ?
carboxyamidomethylated and maleylated lysozyme + H2O
-
 Synechocystis sp. ?
-
 ?
casein + H2O
-
 Synechocystis sp. ?
-
 ?
myelin basic protein + H2O
-
 Synechocystis sp. ?
-
 ?

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 isoform SynPPM3 Synechocystis sp.
7.5 8.4 isoform SynPPP1 Synechocystis sp.