Literature summary for 3.1.3.48 extracted from

Romsicki, Y.; Scapin, G.; Beaulieu-Audy, V.; Patel, S.; Becker, J.W.; Kennedy, B.P.; Asante-Appiah, E.
Functional characterization and crystal structure of the C215D mutant of protein-tyrosine phosphatase-1B (2003), J. Biol. Chem., 278, 29009-29015.

Search for more literature for 3.1.3.48

Activating Compound

Activating Compound	Comment	Organism	Structure
1,2-epoxy-3-(p-nitrophenoxy)propane	C215D mutant, not wild type	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
wild type and C215D mutant, both are structurally identical	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C215D	active-site mutant, similar Km-values as wild type, but reduced kcat-values and activation by 1,2-epoxy-3-(p-nitrophenoxy)propane, use as substrate-trapping mutant	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1	-	p-nitrophenyl phosphate	22°C, pH 6.0, mutant C215D	Homo sapiens
1.4	-	p-nitrophenyl phosphate	22°C, pH 6.0, wild type	Homo sapiens
5.4	-	p-nitrophenyl phosphate	22°C, pH 4.5, mutant C215D	Homo sapiens
5.8	-	p-nitrophenyl phosphate	22°C, pH 4.5, wild type	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P18031	isoform PTP1B	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl phosphate + H2O	-	Homo sapiens	p-nitrophenol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PTP-1B	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	mutant C215D	Homo sapiens
6	-	wild type	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)