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Literature summary for 3.1.3.48 extracted from

  • Gruninger, R.J.; Thibault, J.; Capeness, M.J.; Till, R.; Mosimann, S.C.; Sockett, R.E.; Selinger, B.L.; Lovering, A.L.
    Structural and biochemical analysis of a unique phosphatase from Bdellovibrio bacteriovorus reveals its structural and functional relationship with the protein tyrosine phosphatase class of phytase (2014), PLoS ONE, 9, e94403.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bdellovibrio bacteriovorus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 200 mM MgCl2, 100 mM Tris-HCl (pH 7.0), 8-10% (w/v) PEG 8000 Bdellovibrio bacteriovorus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.574
-
Inositol hexakisphosphate in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus

Organism

Organism UniProt Comment Textmining
Bdellovibrio bacteriovorus Q6MNP0
-
-
Bdellovibrio bacteriovorus HD100 Q6MNP0
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Ni-IMAC column chromatography and Superdex 200 gel filtration Bdellovibrio bacteriovorus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.041
-
using O-phospho-L-tyrosine as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
0.073
-
using ADP as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
0.093
-
using phenolphthalein diphosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
0.14
-
using 4-nitrophenyl phosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
0.2
-
using 1-naphthyl phosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
0.36
-
using D-fructose 1,6-diphosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
1.65
-
using ATP as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
310.8
-
using inositol hexakisphosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O
-
Bdellovibrio bacteriovorus ?
-
?
4-nitrophenyl phosphate + H2O
-
Bdellovibrio bacteriovorus 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Bdellovibrio bacteriovorus HD100 4-nitrophenol + phosphate
-
?
ADP + H2O
-
Bdellovibrio bacteriovorus ?
-
?
ADP + H2O
-
Bdellovibrio bacteriovorus HD100 ?
-
?
ATP + H2O
-
Bdellovibrio bacteriovorus ?
-
?
ATP + H2O
-
Bdellovibrio bacteriovorus HD100 ?
-
?
D-fructose 1,6-diphosphate + H2O
-
Bdellovibrio bacteriovorus ?
-
?
D-fructose 1,6-diphosphate + H2O
-
Bdellovibrio bacteriovorus HD100 ?
-
?
inositol hexakisphosphate + H2O highest activity Bdellovibrio bacteriovorus ?
-
?
additional information the enzyme does not display catalytic activity against some common protein tyrosine phosphatase substrates (O-nitrophenyl beta-D-galactopyranoside, O-phospho-L-threonine, D-fructose 6-phosphate, D-ribose 5-phosphate, D-glucose 6-phosphate, D-glucose 1-phosphate) but is highly specific for hydrolysis of phosphomonoester bonds of inositol hexakisphosphate Bdellovibrio bacteriovorus ?
-
?
additional information the enzyme does not display catalytic activity against some common protein tyrosine phosphatase substrates (O-nitrophenyl beta-D-galactopyranoside, O-phospho-L-threonine, D-fructose 6-phosphate, D-ribose 5-phosphate, D-glucose 6-phosphate, D-glucose 1-phosphate) but is highly specific for hydrolysis of phosphomonoester bonds of inositol hexakisphosphate Bdellovibrio bacteriovorus HD100 ?
-
?
O-phospho-L-tyrosine + H2O
-
Bdellovibrio bacteriovorus ?
-
?
phenolphthalein diphosphate + H2O
-
Bdellovibrio bacteriovorus ?
-
?

Synonyms

Synonyms Comment Organism
Bd1204
-
Bdellovibrio bacteriovorus
protein tyrosine phosphatase
-
Bdellovibrio bacteriovorus
PTP
-
Bdellovibrio bacteriovorus
PTP-like phytase
-
Bdellovibrio bacteriovorus
PTPLP
-
Bdellovibrio bacteriovorus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bdellovibrio bacteriovorus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 55 more than 60% activity Bdellovibrio bacteriovorus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
124
-
Inositol hexakisphosphate in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Bdellovibrio bacteriovorus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5 more than 40% activity Bdellovibrio bacteriovorus