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Literature summary for 3.1.3.48 extracted from
- Staphylococcus aureus contains two low-molecular-mass phosphotyrosine protein phosphatases (2002), J. Bacteriol., 184, 5194-5199.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | both isozymes, 50% inhibition at 7 mM | Staphylococcus aureus |  |
| sodium orthovanadate | both isozymes, 50% inhibition at 3 mM | Staphylococcus aureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
4-nitrophenyl phosphate | pH 6.2, 37°C, PtpA | Staphylococcus aureus | |
| 1.5 | - |
4-nitrophenyl phosphate | pH 6.2, 37°C, PtpB | Staphylococcus aureus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
LMW-PTP, isozymes PtpA and PtpB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein expressed in Escherichia coli | Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.4 | - |
pH 6.2, 37°C, PtpB | Staphylococcus aureus |
| 33.6 | - |
pH 6.2, 37°C, PtpA | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | PtpA, PtpB act specifically on phosphotyrosine residues | Staphylococcus aureus | ? | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Staphylococcus aureus | p-nitrophenol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PtpA | - |
Staphylococcus aureus |
| PtpB | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
PtpA, PtpB | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
PtpA | Staphylococcus aureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 6.5 | PtpB | Staphylococcus aureus |
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Release 2023.1 (January 2023)
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