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Literature summary for 3.1.3.48 extracted from

  • Hoppe, E.; Berne, P.F.; Stock, D.; Rasmussen, J.S.; Moller, N.P.H.
    Expression, purification and crystallization of human phosphotyrosine phosphatase 1B (1994), Eur. J. Biochem., 223, 1069-1077.
    View publication on PubMed
Search for more literature for 3.1.3.48

Cloned(Commentary)

Cloned (Comment) Organism
expression of truncated forms encoding the catalytic domain which is independent of the regulatory influence of the C-terminus Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
phosphotyrosine phosphatase 1B Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information truncated enzyme forms with reduced Km for 4-nitrophenyl phosphate Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
H3VO4
-
 Homo sapiens
heparin
-
 Homo sapiens
molybdate
-
 Homo sapiens
poly(Glu-Tyr)
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 4-nitrophenyl phosphate truncated enzyme b-PTP1B(DELTA321) Homo sapiens
0.1
-
 4-nitrophenyl phosphate truncated enzyme PTP1B(DELTA321) Homo sapiens
0.11
-
 4-nitrophenyl phosphate truncated enzyme b-PTP1B(DELTA299) Homo sapiens
0.38
-
 4-nitrophenyl phosphate
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 50 mM Hepes, pH 7.2, 0.1% 2-mercaptoethanol, 50% glycerol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Homo sapiens 4-nitrophenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
phosphotyrosine phosphatase 1B
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 substrate 4-nitrophenyl phosphate Homo sapiens