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3.1.1.47: 1-alkyl-2-acetylglycerophosphocholine esterase

This is an abbreviated version!
For detailed information about 1-alkyl-2-acetylglycerophosphocholine esterase, go to the full flat file.

Word Map on EC 3.1.1.47

Reaction

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine
+
H2O
=
1-alkyl-sn-glycero-3-phosphocholine
 +
acetate

Synonyms

(PAF)-acetylhydrolase, 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine: acetylhydrolase, 1-alkyl-2-acetylglycerophosphocholine esterase, 1-alkyl-2-acetyllecithin deacetylase, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine acetylhydrolase, 2-acetyl-1-alkylglycerophosphocholine esterase, acetylhydrolase, alkylacetyl-GPC:acetylhydrolase, aspirin hydrolase, blood platelet-activating factor-acetyl hydrolase, Ca2+-independent phospholipase A2, calcium-independent serine lipase Lp-PLA2, deacetylase, 1-alkyl-2-acetyllecithin, group VII PAF acetylhydrolase homolog, group VII phospholipase A2, group VIII phospholipase A2, HpPAF-AH, HSD-PLA2, intracellular platelet-activating factor acetylhydrolase alpha 2 subunit variant 1, intracellular platelet-activating factor acetylhydrolase alpha 2 subunit variant 2, intracellular platelet-activating factor acetylhydrolase alpha 2 subunit variant 3, intracellular platelet-activating factor acetylhydrolase alpha 2 subunit variant 4, intracellular platelet-activating factor acetylhydrolase alpha 2 subunit variant 5, LDL-associated phospholipase A2, LDL-PLA(2), lipoprotein associated phospholipase A2, lipoprotein-associated phospholipase A2, lipoprotein-associated PLA2, lipoprotein-associated platelet-activating factor acetylhydrolase, lipoprotein-PLA2, Lissencephaly-1 protein, Lp-PLA2, LpPLA2, More, PAF 2-acylhydrolase, PAF acetylhydrolase, PAF acetylhydrolase I, PAF acetylhydrolase II, PAF-acetylhydrolase, PAF-acetylhydrolase II, PAF-AH, PAF-AH (II), PAF-AH alpha, PAF-AH I, PAF-AH II, PAFAH, PAFAH alpha, PAFAH1B2, PAFAH2, phosphatide 2-acylhydrolase, phospholipase A2, PLA2, PLA2G7, PLA2G7 gene, plasma lipoprotein-associated phospholipase A2, plasma PAF acetylhydrolase, plasma PAF-AH, plasma platelet - activating factor - acetylhydrolase, plasma platelet activating factor acetylhydrolase, plasma platelet activating factor-acetylhydrolase, plasma platelet-activating factor acetylhydrolase, platelet activating factor acetylhydrolase, platelet activating factor-acetyl hydrolase, platelet activating factor-acetylhydrolase, platelet-activating factor acetyl-hydrolase, platelet-activating factor acetylhadrolase, platelet-activating factor acetylhydrolase, platelet-activating factor acetylhydrolase II, platelet-activating factor acetylhydrolyase, platelet-activating factor acetylohydrolase, platelet-activating factor-acetylhydrolase, platelet-activating-factor acetylhydrolase, Plg7p, pPAF-AH, Serine dependent phospholipase A2, serum platelet-activating factor acetylhydrolase, SpV-1, SpV-2, SpV-3, SpV-4, SpV-5, type I PAF acetylhydrolase, type I platelet-activating factor acetylhydrolase, Type II platelet-activating factor-acetylhydrolase, VIIA phospholipase A2

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.47 1-alkyl-2-acetylglycerophosphocholine esterase

Cloned

Cloned on EC 3.1.1.47 - 1-alkyl-2-acetylglycerophosphocholine esterase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
active enzyme expressed in HEK 293 cells
-
brain isoform I, expression of alpha1 and alpha2 subunits in Escherichia coli, expression of the beta subunit in Sf9 cells
-
cloning of paf-1 gene homologue, expression in Escherichia coli
cloning of paf-2 gene homologue, expression in Escherichia coli
expressed as FLAG-tag fusion protein in COS-7 cells, construction of transgenic mice
-
expressed in Xenopus laevis
-
expression in COS7 cells
-
expression in Escherichia coli
expression in Escherichia coli and in COS cells
-
expression in Escherichia coli BL-21
expression in Escherichia coli BL21 cells
expression of a fragment, corresponding to nucleotides 599-1123 of the human PAF-AH cDNA, quantitative realtime RT-PCR expression analysis in MM6 cells
-
expression of mutant proteins in COS-7 cells
-
expression of N- and C-FLAG-tagged wild-type human PAF-AH, expression of FLAG-tagged human wild-type PAF-AH and of the engineered PAF-AH mutant in Escherichia coli. A region located at the N-terminal end of PAF-AH regulates expression levels
-
expression of N- and C-FLAG-tagged wild-type mouse PAF-AH, expression of FLAG-tagged human wild-type PAF-AH and of the engineered PAF-AH mutant in Escherichia coli. A region located at the N-terminal end of PAF-AH regulates expression levels
-
expression of PAFAH1B2 or PAFAH1B3 subunits in HEK-293T cells
-
expression of the alpha1/alpha2 heterodimer or alpha1 homodimer as His-tagged maltose-binding fusion protein in Escherichia coli XL1-Blue using a bi-citronic expression vector
expression of the catalytic subunit alpha1-homodimer and alpha1-alpha2-heterodimer in Escherichia coli as GST-fusion protein, expression of wild-type and mutant enzymes as GST-fusion proteins in Escherichia coli
-
gene maps to chromosome 6q12.p21.1
-
into pYES-DEST52 using the pENTR/D-TOPO cloning kit, expression in Saccharomyces cerevisiae cells
-
overexpression of isozyme II in HaCaT cells, protects cells against apoptosis induced by tert-butylhydroperoxid, C2-ceramide or ultraviolet B radiation
-
overexpression of the catalytic subunits, especially alpha2, in cultured COS cells induced dramatic phenotypical changes including nuclear shape change, centrosomal amplification and microtubule disorganization
-
PAF-AH isozyme II, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme in Escherichia coli, expression of isozyme II mutant enzymes in CHO-K1 cells
-
plasma isozyme, DNA and amino acid sequence determination and analysis, gene maps at chromosome 6q12-21.1
-
residues 47-429, expression in Escherichia coli cells
transgenic mice overexpressing human PAF-AH II in neurons are generated. Human PAF-AH II expression is found throughout the central nervous system, especially in neurons of neocortex, hippocampus, and basal ganglia